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J Arnaud

Publications and source records attributed to J Arnaud.

167 records · Page 10Linked to original sources
PubMedDate not supplied

On the role of CD3delta chains in TCRgammadelta/CD3 complexes during assembly and membrane expression.

The present study was performed in order to analyze whether T-cell receptor (TCR)/CD3 assembly, intracellular transport and surface expression are carried in a similar way in alphabeta-and gammadelta-T cells. By means of optimal immunoprecipitation conditions with 35S-methionine/cysteine- or biotin-labelled TCR/CD3 proteins from alphabeta- or gammadelta-T-lymphoma-cell lines, as well as TCRgammadelta cDNA transfectants, it was found that CD3delta chains associate less strongly with TCRgammadelta heterodimers compared to TCRalphabeta heterodimers. This preferential reactivity of CD3delta chains appears to be structural and not owing to differences in gammadelta- versus alphabeta-T-cell intracellular environments. Our results are in accordance firstly, with data from CD3delta-deficient mice, which have gammadelta-T cells but no alphabeta-T cells, secondly with the suggested role of CD3delta chains in the positive selection of alphabeta-T cells, a process apparently not followed by gammadelta-T cells, and lastly with the differential roles of CD3delta chains versus CD3gamma chains, explaining the maintenance of two CD3delta and CD3gamma genes after the duplication from a CD3delta/gamma gene present in avians. The impaired reactivity of CD3delta chains with TCRgammadelta heterodimers seems to be owing to a less efficient association with TCRgamma chains. In contrast, CD3delta chains interact as strongly with TCRdelta chains as do CD3gamma chains with both TCRgamma and TCRdelta chains. These data may explain, at the molecular levels, why surface TCR/CD3 expression levels are impaired in gammadelta-T cells from CD3gamma-deficient mice but not from CD3delta-deficient mice.

Animals↗
PubMedDate not supplied

Methaemoglobin and erythrocyte reducing systems in high-altitude natives.

The stress of chronic hypobaric hypoxia present at high altitudes induces a series of adaptive changes in the intermediate metabolism in erythrocytes of high-altitude natives. Aymaras of the high Andean Plateau are shown to have within erythrocytes: (a) increased activity of NADH2 (GAPDH) generating stages, (b) decreased activity of NADH2 (LDH) consuming steps, (c) significantly increased methaemoglobin content, and (d) a large increase in the level of reduced glutathione. These alterations occur also in persons of the same ethnic group residing at low altitude. There is, however, only a moderate elevation of classic haematological parameters (erythrocyte count, haemoglobin and haematocrit) in highland natives. The functional implications of these metabolite changes are discussed with respect to regulation of erythrocyte metabolism.

Acclimatization↗
PubMedDate not supplied

High-altitude haematology: Quechua-Aymara comparisons.

Haematological studies have been carried out at various altitudes between 450 m and 4800 m, on two separate human groups (Quechuas and Aymaras) living in South America. Changes in the haematological parameters do not develop linearly in relation to the attitude. Th impact of chronic hypoxia on erythropoiesis is greater above 3000 m. The haemogram varies quantitatively and not qualitatively (mean corpuscular volume and mean haemoglobin concentration remain constant). The haematological study also reveals the greater adaptability to high altitude of the Aymaras, an adaptability characterized by an increase in red cell count and concentration and a decrease in red cell volume. The adaptative phenomena observed in the Quechuas are reversible, whereas they persist in the Aymaras when they migrate to the lowlands (450 m).

Acclimatization↗
PubMedDate not supplied

Serum cholinesterase polymorphism in France: an epidemiological survey of the deficient alleles detected by an automated micro-method.

The polymorphism of the serum cholinesterase CHE 1 was determined using an automated micro-method. The procedure developed represents an improvement of the manual method and enabled us to study more than 2400 samples obtained in 15 different geographical areas in France. Four alleles were detected: CHE1* U, A, S and F. An exceptionally high frequency of an atypical variant (CHE1*A) was observed in the central part of France (Cevennes, Limousin and Dauphiné). The populations belonging to these areas are at particular risk in case of anaesthesia.

Adult↗