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J Arrese Estrada

Publications and source records attributed to J Arrese Estrada.

At least 19 recordsLinked to original sources
PubMed1993

CD68 and factor XIIIa expressions in granular-cell tumor of the skin.

Immunohistochemical and electron microscopic studies support a Schwann cell origin for most of the granular-cell tumors of the skin. We studied 9 granular-cell tumors by immunohistochemistry, using antibodies to CD68, S-100 proteins, factor XIIIa, L1 antigen, desmin and vimentin. The CD68 monocyte-macrophage-associated lysosomal antigen was expressed in granular cells which however had no Mac 387 reactivity. The 'satellite fibroblasts' were factor XIIIa-positive similarly to dendrocytes and perineurial cells. Our study reveals the lack of specificity of CD68 antibody to identify macrophages. It also presents granular cell tumor of the skin as an exception in the group of schwannomas by the presence of factor XIIIa-positive cells inside the neoplasm.

Adult↗
PubMed1992

Cutaneous manifestations in systemic trichosporonosis.

Trichosporonosis due to Trichosporon beigelii is increasingly recognized in neutropenic immunocompromised patients. We report the clinical and histological presentation of three cases, as well as a study of the prevalence of T. beigelii colonization of normal-looking skin in patients receiving intensive chemotherapy for cancer.

Adult↗
PubMed1992

Immunohistochemical identification of Penicillium marneffei by monoclonal antibody.

We present an experimental study on the immunohistochemical identification of Penicillium marneffei in paraffin-embedded, formalin-fixed tissue. The monoclonal antibody EB-A1 detects a specific galactomannan that appears to have at least one epitope identical in P. marneffei and Aspergillus sp. This immunohistochemical approach could be useful in the diagnosis of a rare diseae, penicilliosis marneffei, which proves to be difficult to identify by conventional microscopy.

Animals↗
PubMed1992

Oral cyclosporin and alopecia areata.

A 30-year-old woman with severe alopecia areata of the scalp was treated with oral cyclosporin for 3 months. Clinical, histological and immunohistochemical assessments failed to reveal any improvement.

Administration, Oral↗
PubMed1992

Dendrocytes in verruga peruana and bacillary angiomatosis.

Verruga peruana and bacillary angiomatosis are two cutaneous diseases characterized by angiomatous growths linked to the presence of rickettsia-like organisms. These lesions are currently considered to be endothelial hyperplasias and to share many features. By immunohistochemistry and computerized image analysis, we studied the presence of factor-XIIIa-positive dendrocytes in these lesions and compared our data with similar research in capillary angiomas and normal skin. Other cell lines were studied by Ulex europaeus and by antibodies to the L1 antigen and to the von Willebrand factor. Dendrocytes were identified in the three types of angiomatous growth. They were numerous and appeared plump, but no more dendritic than in normal skin. Verruga peruana and bacillary angiomatosis should therefore be viewed as combined growths of endothelial cells and dendrocytes. The biological link between these two types of cells is emphasized.

Adult↗
PubMed1992

Nuclear planimetry and DNA flow cytometry of verruga peruana.

Verruga peruana is a vascular hyperplasia that is sometimes histologically mistaken for a malignant neoplasm. An infiltrative pattern with nuclear pleomorphism and mitoses is prominent in some lesions. From a series of lesions of verruga peruana, we selected five atypical lesions, two of them closely resembling epithelioid angiosarcoma. We used histologic criteria as well as morphometry and flow cytometry to further characterize the atypical features of the lesions. Histology and morphometry evidenced marked nuclear pleomorphism. Flow cytometry failed to reveal aneuploidy and disclosed about 10% of the cells to be in the S, G2 and M phase.

Bartonella Infections↗
PubMed1991

Dermal dendrocytes and photochemotherapy.

We studied the fate of dermal dendrocytes in patients treated with psoralens and ultraviolet light by combining immunohistochemistry and computerized image analysis. Factor-XIIIa-positive dermal dendrocytes were found to be altered in these patients. When compared with controls, dermal dendrocytes were often increased in number and had an uneven size and tissue distribution. Their cytoplasm was occasionally fragmented. These changes were more pronounced when early photosclerosis was present. The alterations described probably reflect vascular changes, and may be responsible for immunomodulating actions and disorder of the connective tissue structure induced by ultraviolet light.

Adult↗
PubMed1991

Immunohistochemical expression of epidermal growth factor receptors in nuclei of a subpopulation of keratinocytes and sweat gland cells.

We have raised a polyclonal antibody to the 170-kD epidermal growth factor receptor. We found an intercellular pattern of immunoreactivity in the epidermis as well as a positivity of the cytoplasm of keratinocytes and eccrine secretory cells. In some samples, a nuclear labelling was evidenced in these type of cells. There is a close resemblance in the topographical distribution of these cells with nuclear labelling and those synthesizing DNA under phytohaemagglutinin stimulation.

Cell Membrane↗
PubMed1991

[Immunohistochemistry of Borrelia type spirochetes].

We have looked for the presence of Borrelias by immunohistochemistry in cutaneous biopsies taken from patients with positive Borrelia serology. We used the monoclonal antibody "P41 Borrelia burgdorferi flagellin". We found Borrelias in the dermis of Pick-Herxheimer disease (3/3), erythema chronicum migrans (6/9), lymphocytoma (1/3), and morpheas (2/5). They were also seen in the vascular wall of hypodermal arterioles in a case of cutaneous periarteritis nodosa (1/2). Treponema pallidum was not revealed by the antibody.

Antibodies, Monoclonal↗
PubMed1990

Early dermato-pathological signs during bath-PUVA therapy.

The combination of trioxsalen bath and long-wave UV-A is used in dermatology to treat selected patients with psoriasis. Such treatment induces side effects whose clinical aspects have already been described. We report the histological aspects of these lesions. The most striking features occur in the epidermis, where we found vacuolar alterations, disorganization of the stratum malpighi, necrotic cells, and Bowenoid changes. Langerhans cells are reduced in number, and melanocytes may become large and atypical. In addition, factor XIIIa-positive dendrocytes of the superficial dermis increase in size, and some of them migrate into the epidermis, which is a rare location for these cells.

Adult↗
PubMed1990

Histogenesis of recurrent nevus.

We report an immunohistochemical study of eight cases of recurrent nevi. S-100 protein-positive and Factor XIIIa-negative fibroblast-like cells were found in and around the scar. Some cells were adjacent to vessels whereas others were dispersed in the fibrotic tissue. These cells probably represent entrapped or migrating nevus cells originating from the deep, unremoved portion of the nevus.

Antigens, Neoplasm↗