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J Augustyniak

Publications and source records attributed to J Augustyniak.

28 records · Page 2Linked to original sources
PubMed1977

Leucine transfer RNA from barley. Characterization and purification of isoaccepting species.

Barley embryo leucine tRNA separated on reversed-phase chromatography-5 (RPC-5) into 5 fractions, whereas tRNA isolated from barley seedlings grown both in the light and in the dark, contained 4 species of tRNALeu. Species 2 and 3 were predominant; their relative ratio changed depending upon the growth conditions of the seedlings. Fractionation of crude barley tRNA successively on BD-cellulose, RPC-5 and Sepharose 4B enabled preparative isolation and purification of four leucine isoaccepting tRNAs. The species isolated differed in their main nucleotide composition, melting profiles and MgCl2 titration curves.

Darkness↗
PubMed1976

Large-scale isolation of tRNA from barley embryos.

1. Large-scale isolation of tRNA from barley embryos is described, involving: phenol extraction, RNA deproteinization with the chloroform-isoamyl alcohol mixture, batch sorption on DEAE-cellulose, NaCl gradient elution of tRNA from DEAE-cellulose, and deaminoacylation of tRNA in the presence of bentonite. The procedure yielded tRNA free of protein and RNase activity. 2. The amino acid acceptor activity of the crude barley tRNA, its melting profiles and chromatographic patterns on Sephadex G-100 and BD-cellulose were similar to those of tRNA from other sources.

Amino Acyl-tRNA Synthetases↗
PubMed1974

Isolation and chromatographic behaviour of phenylalanine tRNA from barley embryos.

Two fractions of phenylalanine tRNA (tRNA(Phe) (1) and tRNA(Phe) (2)) were purified by BD-cellulose and RPC-5 chromatography of crude tRNA isolated from barley embryos. Successive RPC-5 rechromatography runs of tRNA(Phe) (2) showed its conversion into more stable tRNA(Phe) (1), suggesting that the two fractions have essentially the same primary structure. Both tRNA(Phe) (1) and tRNA(Phe) (2) had about the same acceptor activity, but tRNA(Phe) (2) was aminoacylated much faster than tRNA(Phe) (1). RPC-5 chromatography of crude aminoacylated tRNA showed higher contents of phe-tRNA(Phe) (2) than of phe-tRNA(Phe) (1) but the ratio of these two fractions estimated by relative fluorescence intensity was about 1. Fluorescence spectra of tRNA(Phe) from barley embryos suggest that it contains Y base similar to Y(w) from wheat tRNA(Phe).

Amino Acyl-tRNA Synthetases↗