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Biomedical subjects

J Ayres

Publications and source records attributed to J Ayres.

10 recordsLinked to original sources
PubMed1979

Antipyretic effect of acetaminophen suppositories in rats.

An animal model used yeast-fevered rats to measure the relative antipyretic effects of different commercially available acetaminophen-containing suppositories. A laboratory-prepared acetaminophen-containing suppository and placebo suppositories also were investigated. Release from the suppositories was measured in vitro. All acetaminophen products containing 600 mg of drug elicited significant decreases in the rectal temperature of fever-induced rats.

Acetaminophen↗
PubMed1979

Saliva, urine and plasma analysis of dyphylline via HPLC.

Improved assay methods for determination of dyphylline levels in plasma, saliva, and urine utilizing high pressure liquid chromatography have been developed. Detection of levels as low as 25 ng/ml from 0.5 ml plasma and 50 ng/ml from 0.5 ml saliva were possible. Both procedures utilized the same extraction process. A separate extraction process was used for dyphylline analysis in urine due to the presence of interferring substances occurring with previous methods. The method was applied to samples of one subjects plasma, saliva and urine after administration of dyphylline.

Chromatography, High Pressure Liquid↗
PubMed1976

HLA-B specificities and w4, w6 specificities are on the same polypeptide.

The human alloantigenic specificities w4 and w6, which are products of a diallelic system genetically associated with the HLA-B locus, have been solubilized by papain digestion of membranes from the lymphoblastoid cell line, RPMI 4265. The w4 and w6 specificities copurified with the HLA-B locus products, HLA-B7 and HLA-B12. Sequential immunoprecipitation experiments were performed using alloantisera to HLA-B7, HLA-B12, w4 and w6, and a purified HLA-B7, B12, w4, w6 antigen pool labeled with 125I-Bolton-Hunter reagent. These experiments demonstrated directly that HLA-B7 and w6, which are genetically associated with each other, are different antigenic determinants on the same molecule, while HLA-B12 and w4, also genetically associated, are distinct antigenic determinants on a second molecule. Arguments are presented which suggest that the HLA-B determinants and w4, w6 determinants are in fact on the same polypeptide, and the genetic implications of the findings are discussed.

Alleles↗