[A study of the fatty acids of platelet phospholipids (author's transl)].
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Biomedical subjects
Publications and source records attributed to J Aznar.
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It was observed that the acetylsalicylic acid "in vitro" (final concentration 10(-4) M) as well as "in vivo" (1 g of aspirin) caused a platelet phospholipids variation which basically consisted of: 1. A diminution of the phospholipids/proteins rate of 22%. 2. A reduction of sphingomyelin "in vivo" of 27.66% and "in vitro" of 16.82%. 3. An increase in phosphatidyl choline "in vivo" of 12.24% and "in vitro" 10.28%. The possible effects that these changes might have on the platelet function are evaluated.
The effect of dipyridamole on platelet phospholipids has been studied. After the platelet incubation with dipyridamole, a 38% reduction in the concentration of the sphingomyelin and a 21% increase in phosphatidyl choline were produced.
The amniotic fluid (AF) when incubated with the patient's own plasma diminishes the lytic activity of the plasma. It is suggested that this inhibition is due to the presence of fibrinolytic inhibitors in the AF. The inhibitors rate increases as pregnancy advances. Evaluating these inhibitors in a group of 65 women before and after the 38th week of pregnancy, a higher rate of fibrinolytic inhibitors is found after the 38th week. The said differences are statistically significant. For the moment it does not seem that the increasing of the inhibitors in the last part of pregnancy might be used as a fetal maturity test.
A streptokinase resistance test (SK-RT) was studied on a group of people from the Mediterranean coast of Spain. It was found that 87.5% of the studied population required a dose of 250,000 U or less to obtain an adequate neutralization of the circulating antibodies. In none of the cases was the required dose higher than 500,000 U. The average dose was of 132,969 U (SD = 102,269). No significant differences were found between the dose required for men and that required for women. A significantly inferior dose to the average was, however, required for people over 50 years old (x = 112,280, SD = 100,459).
The streptokinase resistance test (SRT) has been studied in a group of patients with recent streptococcal infection and/or titres of antistreptolysin higher than 625 units. These patients generally had a higher SRT.
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A method for the evaluation in vitro of platelet-collagen adhesiveness has been developed. This method is based on counting platelets both before and after their contact with an insoluble collagen (IC) suspension. The results are expressed as adhesiveness index (AI). A number of variables which could influence the test has been evaluated and standardized in a way suitable for the routinary use of the technique described.
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