Biomedical subjects

J B Pawliszyn

Publications and source records attributed to J B Pawliszyn.

3 recordsLinked to original sources

PubMed1999

Detection of monocyclic aromatic amines, possible mammary carcinogens, in human milk.

Environmental chemicals may play a role in the etiology of human breast cancer. Aromatic amines, industrial chemicals found as environmental pollutants, have been identified as rat mammary carcinogens. We have detected the monocyclic aromatic amines, aniline, o-toluidine, and N-methylaniline at parts per billion levels in human milk samples, by applying solid-phase microextraction coupled with gas chromatography/mass spectrometry. Our findings indicate that human breast ductal epithelial cells are directly exposed to aromatic amines, including o-toluidine, which is a mammary carcinogen in female rats.

Adult↗

PubMed1998

Solid-phase microextraction of monocyclic aromatic amines from biological fluids.

A solid-phase microextraction (SPME) protocol was developed for the quantitative analysis of monocyclic aromatic amines from biological fluids. The headspace SPME sampling technique was optimized for extraction and concentration of five target analytes (aniline, o-toluidine, 2-chloroaniline, 2,6-dimethylaniline, 2,4,6-trimethylaniline) from urine, blood, and milk. The temperature, pH, and ionic strength of the matrix sample were modified to allow maximum adsorption of the analytes onto the SPME fiber. This method is rapid yet sensitive and can be completed in 15 min on a 5-mL sample. SPME/GC/MS analysis yielded good reproducibility (RSD > 11%) for each analyte from urine, blood, and milk. Method detection limits for the various biological fluids were determined and ranged from 0.40 ppb for 2,4,6-trimethylaniline in urine to 7.7 ppb for aniline in blood. This SPME sampling protocol can be applied to the biomonitoring of monocyclic aromatic amines from occupational, environmental, and medical exposure.

Calibration↗

PubMed1996

Analysis of organochlorine pesticides using solid-phase microextraction.

A solid-phase microextraction (SPME) procedure has been developed to successfully extract eighteen chlorine-containing pesticides from water. A fused-silica fiber coated with a non-polar polydimethylsiloxane stationary phase was used to extract the analytes from water samples over a concentration range of 0.001 to 100 ng/ml. Limits of detection at a ng/l level were achieved with GC and flame ionization detection, and improved to sub ng/l levels using GC-MS, or GC with electron-capture detection (ECD). The reproducibility of the measurements between fibers was found to be very good, with precision typically below 20% R.S.D. within a single fiber. Qualitative analysis was performed on river and lake samples from Southern Ontario. Samples from the Arctic region of Canada were analyzed quantitatively using a dual ECD system.

Arctic Regions↗