Biomedical subjects

J B Senturia

Publications and source records attributed to J B Senturia.

13 recordsLinked to original sources

PubMed1996

Auditory-evoked brainstem responses in the torpid deermouse.

This study examined auditory-evoked brainstem responses (ABR) in the deermouse (Peromyscus maniculatus) during torpor and arousal. The ABR of the euthermic deermouse consisted of five waves occurring in a time frame of 10 ms. During torpor, ABR wave I could be elicited at slow, but not fast stimulation rates indicating variability in neural activity along the auditory pathway. Arousal was heralded by the appearance of all the components of the ABR evoked in response to both slow and fast click rates signaling functional restoration of auditory neural activity during this phase.

Acoustic Stimulation↗

PubMed1994

Antecedent sympathetic stimulation alters time course of chronotropic response to vagal stimulation in dogs.

We determined the influence of antecedent sympathetic stimulation on the chronotropic responses of the heart to 10-s strains of vagal stimulation in anesthetized dogs. We used the reciprocal of the slope (m-1) of the initial portion of the vagal response as an index of the time required for the response to reach steady state. In one group of 11 animals, we found that the onset of the response to the vagal stimulation was progressively blunted as we increased the frequency and duration of the antecedent sympathetic stimulation; that is, m-1 increased from 0 to 8.13 +/- 2.75 (SE) and from 0 to 8.22 +/- 2.26, respectively. In three other animals, an antecedent infusion of norepinephrine had a blunting effect that resembled that of antecedent sympathetic stimulation. In 11 other animals, m-1 significantly decreased as we prolonged the elapsed time from the end of sympathetic stimulation to the beginning of vagal stimulation. In six other animals, m-1 was not affected by antecedent atrial pacing at frequencies that were equivalent to those elicited by antecedent sympathetic stimulations. The blunting effect of antecedent sympathetic stimulation was abolished by propranolol administration (1 mg/kg), but it was not affected appreciably by phentolamine administration (2 mg/kg). We conclude that the major blunting effect of antecedent sympathetic stimulation is mediated postjunctionally (i.e., at the level of the automatic cells in the heart.

Animals↗

PubMed1992

Auditory-evoked brainstem responses in the hibernating woodchuck Marmota monax.

1. This study measured the changes in the auditory-evoked brainstem responses in the woodchuck (Marmota monax) during hibernation and arousal. 2. The auditory brainstem response of the euthermic woodchuck consisted of four waves occurring in a 10 msec time window after stimulation. 3. In the hibernating woodchuck, waves I and II could be traced down to the lowest body temperatures. 4. As temperatures increased all the components of the ABR emerged. The latencies of all the waves showed systematic decrease with temperature increments, the effect being cumulative across the time window. 5. These findings reflect activity in the VIIIth cranial nerve and the cochlear nuclei during hibernation and restoration of the functional integrity of the brainstem auditory pathway during arousal.

Animals↗

PubMed1990

Giardia sp.: comparison of electrophoretic karyotypes.

Species in the genus Giardia have been named on the basis of host specificity, cell dimensions, and median body morphology. Despite these criteria, the species taxonomy of Giardia is still in question. To investigate Giardia taxonomy on a molecular level, Giardia chromosomal DNA was analyzed by orthogonal-field-alternation gel electrophoresis (OFAGE) and transverse alternating field electrophoresis (TAFE). Chromosomal DNA of G. duodenalis isolates (human, muskrat, sheep, dog, beaver), G. muris (mouse), and G. ardeae (great blue heron) were subjected to OFAGE and TAFE analyses. Comparable DNA patterns were obtained by both electrophoretic methods, but OFAGE required 8 days while TAFE required only 3 days. DNA patterns among all G. duodenalis isolates, although quite similar to each other, were distinctly different from those of G. muris and G. ardeae; G. muris and G. ardeae DNA patterns were distinctly different from each other. A G. duodenalis (Portland 1) total DNA probe hybridized to the DNA of all G. duodenalis isolates on Southern blots, but not detectably to G. muris and G. ardeae DNA. Similarly, G. muris and G. ardeae total DNA probes only hybridized detectably to their respective DNA. One probe that appears to hybridize to the DNA of all G. duodenalis and to G. ardeae DNA rather than G. muris DNA has been developed. Another probe that hybridizes only to G. muris and G. ardeae DNA has been developed. These data suggest that the differentiation of Giardia isolated from host and environmental samples may eventually be accomplished by DNA probes. Additionally, these techniques perhaps combined with other criteria may lead to the establishment of a sound taxonomic scheme for this genus.

Animals↗

PubMed1989

Inotropic response elicited by nematocyst contents of Hydra oligactis (Coelenterata: Hydrozoa).

1. Intact, isolated nematocysts from the non-toxic, freshwater coelenterate Hydra oligactis contain soluble material(s) capable of producing a sustained increase in the rate of developed force in the vertebrate myocardium. 2. The positive inotropic effects of this material(s) appear grossly comparable to those described for Anthopleurin-A (AP-A) and Toxin II (ATX-II) from sea anemones. 3. The effects of the nematocyst material are distinct from those of known vasoactive peptides reported to occur in Hydra.

Animals↗

PubMed1986

Serum bactericidal testing with the Autobac system.

Current methodology for the serum bactericidal test requires a minimum of 48 h. A procedure was devised for performing this test with the Autobac system (General Diagnostics, Div. Organon Inc., Raleigh, N.C.) in a shortened time span. All titers obtained with the Autobac were compared against results obtained with a standardized tube dilution procedure. The Autobac low-thymidine eugonic broth performed comparably to the tube dilution diluent, a 1:1 ratio of pooled human serum and cation-supplemented Mueller-Hinton broth (99.2% correlation between bactericidal endpoints). Over 300 tests were conducted by using stock reference bacterial strains, clinical isolates, pooled human serum seeded with antimicrobial agents, and serum from patients on antimicrobial therapy. With the Autobac procedure, serum inhibitory titers can be reported in 3 to 4 h (93.4% correlation with the tube dilution procedure). Serum bactericidal titers can be obtained in 24 h without the necessity of subculturing (95.6% correlation). With the exception of staphylococci tested against penicillin, serum bactericidal titers can be obtained in 3 to 4 h (88.4% correlation). The Autobac procedure can provide the clinical laboratory with a rapid, reliable method for performing the serum bactericidal test.

Anti-Bacterial Agents↗

PubMed1984

Isovolumetric performance of isolated ground squirrel and rat hearts at low temperature.

The effects of low temperature on mechanical performance of the isolated left ventricles of the 13-lined ground squirrel (a hibernator) and the rat (a nonhibernator) were studied. In addition, low-temperature performance of hearts from summer-active, winter-hibernating, and winter-active ground squirrels were compared. By measuring pressure (P) generated against a balloon inserted into the left ventricle, maximum developed pressure (DP) and maximum rate of increase of P (peak dP/dt) were determined over a temperature range of 5-20 degrees C. The DP and dP/dt of the rat ventricle exhibited significantly greater reduction in magnitude at reduced temperature, compared with those of ground squirrel ventricle. Rat, but not ground squirrel, hearts exhibited arrhythmias of various kinds, including extra-systoles, tachycardia, pulsus alternans, and periods of asystole. Hearts from winter-active ground squirrels developed greater pressures than those from winter-hibernating and summer-active animals. This evidence suggests that disruption of cell communication in the nonhibernator ventricular myocardium plays an important role in the failure of the nonhibernator heart at low body temperatures. Contractility of the seasonal hibernator's heart is influenced by both season and hibernation itself, possibly through shifts in myocardial metabolism. However, seasonal adaptations appear not to be required to confer the special resistance of the seasonal hibernator's heart to the deleterious effects of low temperature.

Acclimatization↗

PubMed1983

Ventricular fibrillation in hibernators and nonhibernators.

Previous studies have shown that there are differences between hibernators and nonhibernators in the susceptibility to ventricular fibrillation. In an attempt to clarify these differences ventricular fibrillation was induced in isolated hearts of the hibernator, the woodchuck, Marmota monax by cooling, warming, puncture, and by norepinephrine administration. It was shown that the hearts of the winter animals were completely resistant toward the ventricular fibrillation inducing agents, which was not the case for the hearts from summer, active animals. Further, the hearts of another hibernator, the hedgehog, Erinaceus europaeus, and guinea pig, Cavia porcellus, were studied electrophysiologically in anesthetized animals with open chests and with bipolar electrodes attached to the epicardium. During pacing it was shown that the hedgehog had a higher stimulus threshold and a lower maximal following frequency than the guinea pig. The summer hedgehogs showed resistance toward both ventricular premature beats and ventricular fibrillation. Sixty percent of the summer hedgehogs and 100 percent of the winter hedgehogs and guinea pigs developed ventricular fibrillation. The threshold for ventricular fibrillation was highest for summer hedgehogs. The effective refractory period of papillary muscle of summer hedgehogs was shorter than that of guinea pigs. The force frequency relationship of the isolated papillary muscle showed a greater degree of independence in the hedgehog than in the guinea pig. Consequently, the results show that the heart of the hibernator is more arrhythmia resistant than the heart of the nonhibernator, although there are seasonal differences.

Animals↗

PubMed1974