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Biomedical subjects

J B Trimbos

Publications and source records attributed to J B Trimbos.

At least 91 records · Page 5Linked to original sources

A randomized clinical trial to compare two different approaches in women with chronic pelvic pain.

One hundred six patients with chronic pelvic pain were randomly allocated to one of two treatment groups. In the standard-approach group, organic causes of pelvic pain were excluded first and diagnostic laparoscopy was routinely performed. If no somatic cause could be found, attention was given to other causes such as psychological disturbances. In the second group an integrated approach was chosen. From the beginning equal attention was devoted to somatic, psychological, dietary, environmental, and physiotherapeutic factors. In this group, laparoscopy was not routinely performed. Both groups were similar with respect to clinical characteristics of the patients and the severity of their pain as assessed by various pain parameters. Postcoital pain was reported by 27% of the patients. Twenty percent of the patients had had negative sexual experiences such as childhood sexual abuse or rape. Evaluation of the pain 1 year after the institution of treatment revealed that the integrated approach improved pelvic pain significantly more often than the standard approach for three out of four pain parameters (P less than .01). Laparoscopy played no important role in the treatment of pelvic pain. It is concluded that equal attention to both organic and other causative factors from the beginning of therapy is more likely to result in a reduction of pelvic pain than is a standard approach.

Adolescent↗

Imaging of ovarian cancer with radiolabelled monoclonal antibodies.

This article presents the state of the art of immunoscintigraphy (IS) of ovarian cancer. We will review the monoclonal antibodies (MoAbs) used in clinical trials: (HMFG1/2, OC125, H317, H17E2, NDOG2 and 791T/36). We conclude that none of the afore mentioned MoAbs are clearly superior and that IS cannot yet replace laparotomy for the diagnosis of ovarian cancer but may have a role in the follow-up of ovarian cancer, in timing second-look surgery and assessing the response/TD treatment.

Antibodies, Monoclonal↗

Mechanical performance of square knots and sliding knots in surgery: comparative study.

Various square knots, sliding knots, and surgeon's knots in mono- and multifilament suture material of different diameters were tested with respect to knot strength and knot security. Loop holding capacity of knots varied from 37.2 to 149.8 N, depending on the kind of knot, knot configuration, kind of suture, suture structure, and suture dimension. On the whole, square knots proved to be more reliable than sliding knots. Knot performance of surgeon's knots was not better than that of square knots. Knot security of three-throw sliding knots and two-throw square knots and surgeon's knots was rather poor. These knots cannot be recommended for clinical use. In smaller diameter mono- and multifilament suture material, knot performance of sliding knots that contained one extra throw was identical to that of square knots. It is concluded that the outcome of the comparison of square knots and sliding knots depends on knot configuration, suture material, and suture size. The implications of these laboratory findings for clinical use are discussed.

Evaluation Studies as Topic↗

Conservative management of an ovarian cyst in late pregnancy.

We report a case of concomitant ovarian tumor and pregnancy. Sonographic examination indicated that the tumor was benign and obstructed the birth canal. After conservative treatment (puncture of the cyst), a normal vaginal delivery was possible. Two days after delivery, salpingo-oophorectomy was performed because of torsion of the cyst. Pathological examination confirmed the benign nature of the cyst.

Adult↗

Reasons for incomplete surgical staging in early ovarian carcinoma.

The completeness and associated morbidity of surgical staging in 86 patients with early ovarian carcinoma were analyzed. According to strict criteria for completeness of the procedure established before the onset of the study, surgical staging after one or two laparotomies was complete in only 53% of cases. Initial staging in a peripheral hospital was complete in only 15% of patients. Intraoperative complications occurred in between 8 and 15% of patients and included injury of the vena cava (5), small bowel injury (2), myocardial infarction (1), transection of the ureter (1), and splenic rupture (1). The most frequently omitted staging steps were biopsy of the paracolic gutter, biopsy of the pelvic peritoneum, and sampling of retroperitoneal lymph nodes. Reasons for incomplete surgical staging were divided into factors associated with increased risk of difficulty of the procedure and lack of knowledge of the sites at risk for ovarian cancer metastases. It was found that both phenomena should be held responsible to the same extent for the large number of incomplete surgical staging procedures. The conclusion was made that either gynecologists should be better educated in the staging of ovarian cancer or a more efficient patient referral policy should be considered.

Adult↗

Should the Cytobrush be used in routine screening for cervical pathology?

In 632 women visiting a family planning clinic, paired cervical smears were taken using a wooden spatula and a Cytobrush. Of the spatula smears 7.8% were unreliable with too little or scanty material to allow a cytologic diagnosis (class 0); 17.7% of spatula smears were inadequate, lacking endocervical cells. Combined spatula/Cytobrush cytology reduced these figures significantly; 4.3% unreliable smears and 3.2% inadequate specimens. Seventy-three (11.6%) of the smears revealed some abnormality, in most cases Papanicolaou class IIIA, suggestive of mild dysplasia. In 10 cases, dysplastic cells were found only in the Cytobrush cytology. It is concluded that the Cytobrush adds to the improvement of smear quality and the accuracy of routine cervical cytology obtained by relatively inexperienced smear takers.

Contraceptives, Oral↗

Factors relating to the volume of surgical knots.

The suture's knot site represents the highest amount and density of foreign body material of a knotted suture loop. The volume of a knot is directly related to the total amount of inflammatory reaction surrounding the knot. We measured the volume of seven surgical knots frequently used in surgical practice. Two different suture materials and two different sizes were tested. Suture size was found to contribute significantly more to the volume of all knots tested than the number of throws of which the knots were composed. Knots made with coated multifilament polyglactin-910 were significantly larger than the knots made with monofilament nylon. Clinical implications of these findings are discussed. Surgical techniques keeping the volume of knots to a possible minimum, are encouraged.

Foreign-Body Reaction↗

Piperacillin for prophylaxis in gynecological surgery.

The effectiveness of perioperative Piperacillin for prevention of operative-site infection after gynaecological surgery was studied in a total of 100 patients. In an open randomized clinical trial of 52 women undergoing abdominal or vaginal hysterectomy Piperacillin was compared to the combination Gentamicin/Metronidazole. Piperacillin was well-tolerated. Surgical-site infection was diagnosed in the Piperacillin group in two patients and in the Gentamicin/Metronidazole group in one patient. The occurrence of postoperative febrile morbidity was also similar between the two group. In 48 patients undergoing complicated gynecological surgery of long duration, only Piperacillin was administered perioperatively. Thirty-five of these 48 women underwent oncological operations and the mean operative length was 3.3 h. One patient developed a transient rash after the administration of Piperacillin. No other adverse side-effects were observed. Nine of these 48 patients developed a surgical-site infection postoperatively, and this was found to be significantly more often than in the first group of 52 patients. It was concluded that Piperacillin seems to be a valuable alternative for perioperative prophylaxis in gynecological surgery. Its effectiveness in vaginal and abdominal hysterectomy is similar to the combination of Gentamicin/Metronidazole, but after complicated surgery of long duration a decreased effectiveness should be reckoned with.

Bacterial Infections↗

Tissue reaction and surgical knots: the effect of suture size, knot configuration, and knot volume.

Tissue reaction surrounding surgical knots was assessed histologically using a semiquantitative scoring system in 40 male Wistar rats. We tested sizes 2/0 and 4/0 (United States Pharmacopeia [USP]) coated polyglycolic acid and coated polyglactin-910 sutures, and two different knots. In addition, we measured the volume of the different knots and the total volume of tissue reaction surrounding the knots. Multivariate analysis showed only suture material and suture size to be independent factors determining tissue reaction. An increase of suture size resulted in a more than four- to sixfold increase in knot volume and a more than two- to threefold increase in the amount of the tissue-reaction sheath; however, the addition of extra throws to the knot enlarged the knot body by a factor of ony 1.5 and the tissue-reaction sheath by a factor of 1.0-1.9. These findings suggest that the use of thick-gauge suture material adds much more to the total amount of foreign body and tissue reaction in the wound than the addition of extra throws to the knot and might, therefore, be deleterious to optimum wound healing.

Animals↗

Assessment of tissue reaction at suture knots; an adaptation of Sewell's scoring system.

From a theoretical point of view and from reported animal work the knot side should be considered the most relevant part of the suture loop for studying inflammatory foreign body reaction. In the present study a semi-quantitative histological assessment of inflammatory tissue reaction around the knots of implanted sutures is presented. To test the reliability of this method an animal model was used in which two suture materials with well-known differences in tissue reactivity were studied. Differences in tissue response around the knots between these materials proved to be highly significant statistically (tissue reactivity score (TRS) 44.17 vs. 58.71: p less than 0.001). No differences in TRS could be demonstrated between different localizations in the anterior abdominal wall of male Wistar rats. Implications of these findings with respect to future studies are discussed.

Abdominal Muscles↗

The treatment of advanced ovarian carcinoma (I): clinical variables associated with prognosis.

New therapy regimens including cytoreductive surgery and cisplatin-based combination chemotherapy have improved survival for a small group of patients with advanced ovarian carcinoma. However, for the entire group survival remains poor. In this analysis of 65 patients treated at Leiden University Medical Center, the patient group that benefited the most from this approach was characterized by a younger age, good performance status, less extensive disease, low histologic grade and the absence of peritoneal carcinomatosis and ascites, and residual disease less than or equal to 15 mm (successful cytoreduction) after the first operation. Patients who did not have these favorable characteristics but in whom the first operation resulted in residual disease less than or equal to 15 mm, did survive worse than the other patients with individual tumor nodules less than or equal to 15 mm, but still better than those in whom a successful cytoreduction at the first operation could not be achieved. The diameter of the largest residual disease, the diameter of the largest metastasis before cytoreduction, and the presence of ascites and peritoneal carcinomatosis influenced prognosis. Thus the initial tumor burden as well as the burdened tumor volume left behind are of significance with respect to prognosis. However, in the Cox regression analysis the performance status and ascites proved to be the only independent factors influencing survival. A prospective study is needed to establish the proper value of cytoreductive surgery in advanced ovarian carcinoma.

Altretamine↗

The role of cytoreduction in ovarian cancer.

Whether or not cytoreduction improves the outlook of ovarian cancer patients is not yet clear from data from Literature. Maybe not the successful procedure itself, but the favourable tumor characteristics of these tumors determine the prognosis. New studies are necessary to define the role of surgery in the overall management of ovarian cancer more precisely. In this article a Dutch study is presented, to answer the question about the influence of attempted cytoreductive surgery prior to, during chemotherapy on survival time and remission rates. All patients are treated with either combination chemotherapy and/or an attempt to cytoreduction prior to or during chemotherapy. With this strategy ethical problems concerning undertreatment are avoided. The study is currently activated but it is too early to draw any conclusions.

Altretamine↗

Performance of the Cytobrush in patients at risk for cervical pathology: does it add anything to the wooden spatula?

In 130 high-risk patients for cervical pathology paired smears were obtained with a wooden spatula and a Cytobrush. The Cytobrush was useful in lowering the amount of inadequate smears (2 vs 22%, p less than or equal to 0.01), and detected more abnormal cytology in combination with the spatula compared to the spatula smear alone (27 vs 35%, p less than or equal to 0.05). In 6 cases, adequate false-negative spatula smears, containing endocervical cells, were corrected by the Cytobrush. It is concluded that the Cytobrush is useful in lowering the amount of inadequate smears and false-negative cytology, while there is little evidence of false-positive cytology. The use of the wooden spatula/Cytobrush-combination is recommended in high risk populations.

Adenocarcinoma↗

Explanation of the limited correlation between tumor CA 125 content and serum CA 125 antigen levels in patients with ovarian tumors.

The concentration of the tumor marker CA 125 in tumor tissue, cyst fluid, ascites fluid, and serum from patients with epithelial ovarian tumors was quantitated. Immunohistologic studies showed that CA 125 was present in 90% of the nonmucinous epithelial ovarian tumors. Quantitative analysis of the fluid from 57 cysts revealed that CA 125 was present in concentrations of up to 2140,000 U/ml in samples from malignant nonmucinous epithelial ovarian lesions, and up to 116,000 U/ml in mucinous tumors, but also in concentrations of up to 371,000 U/ml in benign serous cystadenomas. In contrast, pre-operative serum CA 125 levels were elevated in almost all of the patients with malignant ovarian tumors but not in most of those with benign ovarian tumors. These findings suggest that in benign ovarian tumors there is an effective barrier between the cyst fluid and the circulation that prevents the appearance of CA 125 in the serum, whereas in malignant tumors infiltrative growth leads to the release of antigen into the circulation. Furthermore, CA 125 values in ascites fluids were up to 130 times higher than the serum antigen levels, which indicates that the peritoneum serves as a barrier for high molecular weight tumor antigens. The current results show that tumor basement membranes and peritoneal barriers play a notable role in the transit of tumor antigens, one which must be taken into account in the monitoring of serum marker levels of cancer patients.

Antigens, Neoplasm↗