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Biomedical subjects

J Bílek

Publications and source records attributed to J Bílek.

At least 19 recordsLinked to original sources
PubMed1992

[Arterial injuries of the upper extremities].

At the Department of Surgery in Pilsen in 1982-1991 a total of 26 patients with upper extremity vascular injuries was treated. Brachial artery was injured most commonly (50%). The direct trauma was responsible for 88.5% injuries, indirect injury was recorded in 11.5% cases only. The incidence of concomitant injuries of the vein, nerve or bone was 73.1%. Approximately one third of arterial injuries was caused by criminal offences. The vascular reconstruction was performed in all 26 patients. The overall patency rate was 84.6%, the amputation rate was 3.8%. One patient with polytrauma died of sepsis (3.8%). The authors give a comprehensive review of the causes, diagnosis and treatment of vascular injuries of the upper extremities.

Adolescent↗
PubMed1992

[Extra-anatomic bypasses in reconstructive vascular surgery in the lower extremities].

Extra-anatomic Bypass was used in 36 patients for limb salvage at the Department of Surgery, University Hospital in Pilsen in a period of almost eleven years (1th January 1981-1th November 1991) constituting 8.2% of all vascular reconstructions. Amputation rate was 22.2% and mortality rate 8.3% (without direct connection with reconstruction). Extra-anatomic reconstruction was used in such cases only where the anatomic bypass was excluded for general or local causes.

Adult↗
PubMed1990

[Aneurysms of the peripheral arteries].

The authors discuss the problem of the right peripheral arterial aneurysm. In 1985-1989, five patients with peripheral arterial aneurysm were operated on at the surgical department, teaching hospital. Plzen. The paper gives an account of each case pointing to the specific features of this particular part of vascular surgery. Further, the authors describe the etiology, diagnostics and treatment of the disease.

Aged↗
PubMed1989

[Secondary access routes for hemodialysis].

During the period of 1970-1985 375 arteriovenous fistulae were constructed in 248 dialyzed patients. The number of fistulae needed for long-term dialysis was significantly higher in women. Five-year cumulative patency rates were significantly better in the first fistulae than in the second, third or following fistulae. The best long-term patency rates were achieved in radiocephalic fistulae. Of all secondary vascular access procedures saphenous vein grafts proved to be the most reliable.

Adolescent↗
PubMed1989

[Symptomatic aneurysms of the abdominal aorta].

In the years 1981-1985 21 patients were operated upon for symptomatic abdominal aortic aneurysms. Atypical symptoms such as acute pancreatitis, weight loss, blood in the stool or urine, incorrect interpretation of abdominal pain and physical examination findings caused erroneous initial diagnosis and delay in referring the patient to surgical treatment in nine cases. Long history prior to admission was noted even in patients with ruptured aneurysms; mortality in these patients was high, 66%. Best results were achieved with aneurysm resection; indirect aneurysm occlusion in combination with axillofemoral bypass attempted in two patients was not successful.

Aged↗
PubMed1984

[Methods of diagnosing subclinical mastitis in dairy cow mastitis control programs].

The number of somatic cells and the isolation of the causative agents of mastitis in quarter, composite, bucket, and bulk tank samples of cow's milk was determined four times during a six-month period. The number of somatic cells in milk samples indicated a degree of mastitis infection and was influenced neither by the year season nor by the length of lactation. At a repeated examination of 28 dairy cows an increased number of somatic cells in milk was found once in 68 udder quarters and with three successive samplings only in 21 quarters. The etiological agents of mastitis were detected once in 31 quarters and three times in succession only in five quarters. The number of cows positive by the number of cells in quarter samples of milk increased from 52.9-58.8% at a single examination to as much as 100% at four examinations. The etiological agents of mastitis were isolated in a single examination in 17.6% of cows and at four examinations in 58.8% of cows. The composite and bucket samples of milk containing 200 to 300 thousand cells per ml are recommended to be considered as mastitis-positive: in 68 to 78% they came from cows having more than 500 thousand cells per ml at least in one quarter sample. The number of cells in a bulk sample was in correlation with the percentage of cows having a positive NK-test (similar to CMT) and positive isolation of S. agalactiae from quarter milk samples.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1983

The proportion of fatty acids in mammary secretion of cows lactating after calving and hormonal induction of lactation.

The changes in the proportion of fatty acids in lacteal fat of cows induced into lactation by 7-day treatment with oestradiol and progesterone were characterized by an increases in the proportion of fatty acids C6--C13: the proportions of C14, C15, C17, C19 fatty acids changed slightly if at all; the proportions of C16, C20 and C22 fatty acids decreased during the first 28 days of lactation. Similar changes in fatty acid composition were found in lacteal fat of cows lactating after calving. These results demonstrated that the hormonal induction of lactation in nonpregnant cows initiates mammary secretion similar to the fatty acid composition in normal cow milk.

Animals↗
PubMed1982

Effect of progesterone, triiodothyronine and metabolic inhibitors on prolactin stimulated lipid synthesis in goat mammary explants.

The effect of progesterone, 3,3',5-triiodo-L-thyronine (L-T3) and of inhibitors of DNA, RNA and protein synthesis on prolactin stimulated total lipid synthesis in goat mammary explants were studied. Prolactin stimulated lipid synthesis was not affected by the addition of progesterone. The synthesis of lipids in the presence of insulin, cortisol and prolactin was not stimulated by L-T3 (10(-9) mol 1(-1)) added at start of culture. However, when the addition of L-T3 was delayed by 48 h following culture with insulin, cortisol and prolactin, the synthesis of lipids was increased between 96--99 h of culture by 48%. This effect of L-T3 was blocked by the inhibition of RNA synthesis with actinomycin D. The inhibition of DNA synthesis by hydroxyurea or cytosine-arabinofuranoside had no effect on prolactin stimulated lipid synthesis in goat mammary explants. Actinomycin D, cycloheximide and puromycin completely blocked prolactin stimulated lipid synthesis when added to culture medium simultaneously with prolactin.

Animals↗
PubMed1982

Artificial induction of lactation in dry cows.

Oestradiol, progesterone, cortisol and reserpine were utilized in treatment regimes for hormonal induction of lactation in dry nonpregnant cows with previous reproductive failures. Lactations were successfully induced in 44 out of 55 cows. The udders of cows in which lactation had been induced successfully were visibly enlarged after 7 days of oestradiol-progesterone treatment on experimental day 8-14 and hence before the administration of cortisol (day 18, 19 and 20). It was concluded that addition of cortisol to oestradiol-progesterone treatment does not improve the success rate of hormonal induction of lactation. The addition of reserpine to oestradiol-progesterone treatment improved the success rate of hormonal induction of lactation. The reduction of oestradiol-17 beta dosage from 60 mg to 30-40 mg day-1 with progesterone at 150 or 200 mg day-1 for the initial 7 days increased both milk yield and the success rate of the hormonal induction of lactation and eliminated pelvic fractures and excessive oestrous activity in treated cows.

Animals↗
PubMed1982

Hormonal control of casein synthesis in mammary explants from pregnant goats.

The effects of insulin, cortisol, prolactin, 3,3',5-triiodo-L-thyronine (L-T3) and progesterone on the synthesis of total protein and casein in mammary explants from pregnant goats were studied. In the absence of hormones and in the presence of insulin plus cortisol the rate of incorporation of 14C-leucine into proteins that were precipitated with the anti-casein antibody decreased during culture. The addition of prolactin to hormonal combination of insulin and cortisol caused large stimulation of rates of casein synthesis. Maximum incorporation of leucine was attained between 3 and 5 days of culture in the presence of 0.5 microgram ml-1 of prolactin. Prolactin stimulated-casein and total protein synthesis were not consistently affected by the addition of L-T3 or progesterone. The inhibition of DNA synthesis by hydroxyurea or cytosine-arabinofuranoside had no effect on casein synthesis in mammary explants from pregnant goats.

Animals↗
PubMed1982

Effect of bovine growth hormone on development of goat mammary tissue in organ culture.

The effect of bovine growth hormone (bGH) on DNA, protein and casein synthesis in goat mammary explants was studied. Growth hormone was unable to stimulate DNA synthesis or potentiate insulin-stimulated DNA synthesis either in high or low oxygen concentrations. In the presence of insulin and cortisol bGH had no effect on the synthesis of explant cytosol proteins. Two preparations of bGH were tested for their effect on casein synthesis. The preparation NIH-GH-B17 at concentration 50 micrograms ml-1 increased casein synthesis similarly as about 0.5 micrograms ml-1 of prolactin. Our preparation, prepared by the method of Prusík and Braun [1967], at concentration 50 micrograms ml-1 had effect lower than 0.05 micrograms ml-1 of prolactin. The lactogenic activity of NIH-GH-B17 was decreased by treatment with specific antisera to bovine prolactin. When submaximum concentration of prolactin (0.05 microgram ml-1) was added, bGH at concentration as low as 0.5 microgram ml-1 had synergistic effect on prolactin stimulated casein synthesis.

Animals↗