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Biomedical subjects

J Bücking

Publications and source records attributed to J Bücking.

15 recordsLinked to original sources
PubMed2002

Endometrial interleukin-6 in vitro is not regulated directly by female steroid hormones, but by pro-inflammatory cytokines and hypoxia.

Endometrial interleukin-6 (IL-6) mRNA has been reported to be suppressed in the mid-secretory phase in patients with recurrent early spontaneous abortions. This prompted our study concerning the regulation of endometrial IL-6 in cell culture models of endometrial epithelial and stromal cells. Steroid-dependent secretion of IL-6 was analysed by 17beta-estradiol (10(-8) mol/l) or progesterone (10(-6) mol/l) treatment and withdrawal (n = 8). Regulation by pro-inflammatory cytokines was studied in co-cultures of endometrial cells with human blood peripheral mononuclear cells (PBMC; n = 5) and by stimulation with IL-1beta, IL-6 and tumour necrosis factor alpha (TNFalpha), secreted by PBMCs at high concentrations. Regulation by hypoxia was assessed by culture of endometrial cells in 2% oxygen for 6 and 24 h (n = 5). IL-6 mRNA and protein levels were analysed by RT-PCR and enzyme-linked immunosorbent assays respectively. Endometrial IL-6 was not directly affected by 17beta-estradiol and/or progesterone. Co-culturing endometrial cells with PBMCs led to an increase of stromal but not epithelial IL-6 mRNA levels. In stromal cells, IL-6 secretion increased 2-10-fold if stimulated with 10 ng/ml recombinant IL-1beta or TNFalpha (P < 0.05). Hypoxia stimulated IL-6 secretion in epithelial cells up to 2-fold and in stromal cells up to 48-fold (P < 0.05). In conclusion, IL-6 expression in stromal and epithelial cells in vitro is regulated differently by pro-inflammatory cytokines and hypoxia. These results suggest a tight and specific network of control for this important cytokine within different endometrial compartments.

Cell Culture Techniques↗
PubMed1986

[Swimming after myocardial infarct. Oxygen saturation, pulmonary arterial and capillary pressure in underwater immersion and during swimming after myocardial infarct].

Pulmonary arterial (PA) and pulmonary capillary (PC) pressures and oxygen saturation were measured over a 5-minute period on immersion in water and during swimming in four symptom-free men (aged 52-58 years) who had sustained a transmural infarct 6-10 weeks previously. Measurements were made through an indwelling flow-guided percutaneously introduced catheter. The speed of swimming was 20-25 m/min. Static pressures on immersion up to the neck rose up to 23 mm Hg, on diving to five times as much above the resting recumbent value. Mean PC values after five minutes of swimming rose in all four subjects above those obtained after a 5-minute 100 W exercise: they were similar to the diastolic pressures. But the oxygen saturations on swimming were in all patients higher than after tread-wheel exercise. All patients felt swimming to be easier than the ergometric exercise. It is concluded that noninvasive methods, such as X-rays, ergometry and echocardiography, are not sufficient for estimating volume stress induced by immersion in water. Cardiac arrhythmias did not occur.

Exercise Test↗
PubMed1984

[Pacemaker therapy with a steroid-containing electrode. Better conditions for stimulation and detection by steroid release from the tip of the probe].

Threshold increase, regularly observed after implantation of pacemakers, leads to increased current consumption and is the decisive factor in determining duration of pacemaker function. Local liberation of dexamethasone at the catheter tip inhibits reactive scar formation at this point. Studies on ten patients showed that with such a steroid-containing catheter thresholds even four weeks after implantation are below 0.07 ms at 2.5 or 1.25 V. Intraoperatively measured intracardiac R-wave potentials increased from 8.4 to 12.5 mV.

Aged↗
PubMed1983

[Intramyocardial block in carbamazepin poisoning].

A 37-year-old woman took 20 g carbamazepine with suicidal intent. After 16 hours the serum carbamazepine level was 260 mg/l. She died 29 hours after taking the tablets. Main cause of death was progressive intramural conduction disturbance. As carbamazepine is widely used, it should also be considered in intoxications associated with intraventricular and intramyocardial conduction disturbances. Haemoperfusion is suggested as a means of secondary poison elimination.

Adult↗
PubMed1983

[Treatment of life-threatening digoxin poisoning with heterologous digoxin-specific antibody fragments].

A 72-year-old woman was admitted to hospital four hours after taking, with suicidal intent, 20 mg digoxin (100 tablets Novodigal) and 50 mg nitrazepam (10 tablets Mogadan). No digoxin tablet residues were found on gastroscopic gastric lavage. A pacemaker was placed transvenously into the apex of the right ventricle. Multifocal ventricular premature systoles and recurrent ventricular fibrillation, which was difficult to terminate, alternating with extreme bradycardia quickly responded to the administration of heterologous digoxin-specific antibodies. Five hours after treatment had been started (total dose 800 mg antibody fragments) the patient again had a stable sinus rhythm of normal rate.

Aged↗
PubMed1983

[Long-term course of primary vascular pulmonary hypertension with and without intake of appetite depressants].

1. The long-term follow-up of 22 patients with anorectic drug intake (aminorex fumarate) (AS) and PVPH is compared to 38 patients with PVPH of unknown etiology. 2. The 10-year cumulative survival rate is significantly higher for the AF-positive group (54 +/- 11%) than for the AF-negative patients (15 +/- 6%). 3. In terms of the hemodynamic parameters at the time of diagnosis, there were no differences between the two patient groups. 4. Hemodynamic serial controls in the AF-positive group often show a decrease in pulmonary pressure at rest, in contrast to the AF-negative group. However, in all but one of the patients with a pressure drop at rest there is a considerable increase in pulmonary pressure during exercise. 5. In neither group any correlation is to be found between pulmonary pressure and survival time.

Adolescent↗
PubMed1981

[Experience with special-type pacemaker leads (author's transl)].

The dislodgement rate of endocardially placed pacemaker leads can be decreased by the use of special-type electrodes. Since 1963 a total of 2100 pacemaker implantations was performed. Since 1970 we have been using special-type electrodes in 15 to 20% of our patients each year. The complication rate of all pacemaker leads implanted in the period 1974 to 1978 was 7.6%, the rate of the special type electrodes alone came up to 15%. The lead IE--65--I had the greatest deal of this - 11 out of 43 leads had to be removed. 152 electrodes type IVE--85--armed with metal hooks - were implanted, 18 of which had to be revised and 17 of 117 rigid leads type IE--60--KS--10 revealed failure which made revision necessary. In contrast, 54 screw-in leads type 6957, Medtronik and AE--60--YR, Biotronik caused only one perforation - a lethal one. Primary experiences with a porous tip lead (CPII--4116) are good enough to recommend the use of this electrode as a lead of first choice. We saw 2 dislodgements in 50 implantations. Failure to sense or to pace were never seen (time of observation 2 to 18 months).

Arrhythmias, Cardiac↗
PubMed1980

[Early and late complications of endocardial pacemaker wires (author's transl)].

Comparison of early and late complications of 769 endocardial pacemaker wires implanted in the period between 1969 and 1973 with 861 wires implanted between 1974 and 1978 revealed a decline of the complication rate from 17% to 7.6%. The dislocation rate could be lowered from 6.2% to 1.6%. Other early complications (exist and entrance block) were not effected (3.5% as compared with 3.2%). Late complications declined from 6.2% to 2.9%. Of these the number of intravascular wire breakages constitutes a high proportion. The main reason for this improvement is the use of a flexible Flange-tip lead. A variation of testing the positional stability of the wire is described.--The indications for pacemaker implantation shifted from 3rd degree AV-Block to the diagnoses "pathological bradycardia" and "hypersensitive carotis sinus". The comparatively low complication rate permits the application of new types of wires only in a few selected patients until a sufficient number of cases and an adequate follow-up period have been reached for clinical evaluation of these wires.

Aged↗
PubMed1980

[Clinical correlations, lactate extraction, coronary venous bloodflow and Thallium-201 myocardial imaging in patients with isolated left anterior descending muscle bridges: normal variant or obstruction? (author's transl)].

In 848 coronary arteriograms performed in a two-years period 21 patients (2.5%) showed a myocardial bridging of the left anterior descending artery. Resting- and/or stress-ECG were abnormal in half of the patients. Regional lactate-metabolism measured in the great cardiac vein at rest and during maximal atrial pacing was normal (29 +/- 12 resp. 24 +/- 9%). Thermodilution of great cardiac vein bloodflow at rest and during atrial pacing also demonstrated normal values (94 +/- 33 resp. 138 +/- 30 ml/min). Biphasic 201-Thallium myocardial imaging revealed no case of reversible perfusion defect, but surprisingly frequent (5 of 16 patients) clearly irreversible defects limited to the interventricular septum. We conclude that muscle bridges do not cause myocardial ischemia at rest or during exercise.

Coronary Circulation↗
PubMed1976

[Evaluation of anti-D prevention using the optical mark page reader].

Based on our experiences with the optical mark page reader-formular we can say that, although this method can be used in clinical routine, it is very difficult to prepare it in such a way that the interesting data are really collected. In our example a great number of data had to be put in retrospectively based on the clinical notes, so that additional work resulted. In our opinion it is impossible to ovoid the addition of data by hand even with careful planning. A progressive adaptation of computer forms to clinical needs might be a possibility to replace classical clinical notes by computer forms. This method, however, is applicable for the analysis of well defined and statistically interesting data, when the scientific questions are clearly defined. Anti-D-prophylaxis is described as a sample using computer forms. At the same time, our results with the Anti-D-prophylaxis are reviewed.

ABO Blood-Group System↗