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Biomedical subjects

J Bardwell

Publications and source records attributed to J Bardwell.

9 recordsLinked to original sources
PubMed2006

Listeria outbreak associated with sandwich consumption from a hospital retail shop, United Kingdom.

An outbreak of listeriosis occurred in the Swindon area of the UK in autumn 2003. Five cases were detected in pregnant women. Four of these women were thought to have eaten prepacked sandwiches from a retail outlet in one particular hospital. Sampling at the supplier detected Listeria monocytogenes, which was indistinguishable on molecular testing from the patients isolates. Recent changes in UK food legislation should help diminish the risk of further outbreaks/cases such as ours occurring.

Disease Outbreaks↗
PubMed1999

The relationship between diabetic amputees and vascular reviews.

This paper reviews the data in the literature pertaining to risk factors for amputation in the diabetic population, and describes a retrospective study to assess the vascular and other assessments carried out on a sample of 50 diabetic patients undergoing minor (toe and foot) and major (below-knee and above-knee) amputations. The study found that 50% of the patients did not have Doppler studies, 52% did not have angiography, and 78% did not have angioplasty. Major amputations were performed in 76% of the sample. This is an unacceptably high figure. Thorough vascular assessments need to be performed in an attempt to save lower limbs from potential amputation.

Aged↗
PubMed1998

Reconstitution of a protein disulfide catalytic system.

Disulfide bonds are important for the structure and stability of many proteins. In prokaryotes their formation is catalyzed by the Dsb proteins. The DsbA protein acts as a direct donor of disulfides to newly synthesized periplasmic proteins. Genetic evidence suggests that a second protein called DsbB acts to specifically reoxidize DsbA. Here we demonstrate the direct reoxidation of DsbA by DsbB. We have developed a fluorescence assay that allows us to directly follow the reoxidation of DsbA. We show that membranes containing catalytic amounts of DsbB can rapidly reoxidize DsbA to completion. The reaction strongly depends on the presence of oxygen, implying that oxygen serves as the final electron acceptor for this disulfide bond formation reaction. Membranes from a dsbB null mutant display no DsbA reoxidation activity. The ability of DsbB to reoxidize DsbA fits Michaelis-Menten behavior with DsbA acting as a high affinity substrate for DsbB with a Km = 10 microM. The in vitro reconstitution described here is the first biochemical analysis of DsbB and allows us to study the major pathway of disulfide bond formation in Escherichia coli.

Catalysis↗
PubMed1996

Diabetic neuropathy.

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Community Health Nursing↗
PubMedDate not supplied

For good measure.

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Blood Pressure↗