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Biomedical subjects

J Bas

Publications and source records attributed to J Bas.

25 records · Page 2Linked to original sources
PubMed1987

Age-dependent effects of acute and chronic intermittent stresses on serum metallothionein.

Prepuberal and adult male rats were chronically stressed for a month with several acute stressors in a random schedule. Some of the animals were killed approximately 20 hours after the last stress session without any additional stress. Other animals from both control and chronic stress groups were subjected to an acute restraint stress for 15 min before being killed. While chronic stress did not alter hepatic metallothionein (MT) either in prepuberal or in adult rats, this treatment significantly increased serum MT levels in young but not in adult rats. Likewise, 15 min of restraint stress increased serum MT levels in young rats only, regardless of whether they were control or chronically stressed rats. The present data indicate that acute and chronic stressors may alter serum MT in an age-dependent fashion.

Acute Disease↗
PubMed1986

Metallothionein in the liver of the small lizard Podarcis muralis.

A cysteine-rich protein presenting optical and biochemical features typical of metallothionein and a similar amino acid composition was found in the liver of the small lizard Podarcis muralis. Animals were given either CdCl2 (0.8 mg Cd2+/kg body wt) or saline (NaCl 0.9%) by i.p. injection for 3 days. A second group of animals were injected with a single dose of [35S]cysteine plus CdCl2 or saline. Lizard MT contained Zn and Cu when injected with saline and also Cd when injected with CdCl2. Metallothionein induction by cadmium was demonstrated by radioactive labelling.

Amino Acids↗
PubMedDate not supplied

Hypersensitivity reaction after orgotein (superoxide dismutase) administration.

Orgotein is being increasingly used in the treatment of some inflammatory disorders. Up to now no hypersensitivity reaction has been reported. We present the case of an allergic reaction demonstrated by both, "in vivo" and "in vitro" tests. This finding further supports the need for an adequate control during and after orgotein administration.

Adult↗
PubMedDate not supplied

In vitro donor-specific hyporesponsiveness and T cell subsets in renal allograft recipients.

In order to assess the immune mechanisms triggered by an immunosuppressive regimen consisting of prophylactic antilymphocyte globulin plus low-dose cyclosporine A and steroids, we studied the short-term evolution of both, anti donor in vitro alloresponse and peripheral blood T cell subsets in 21 recipients of a cadaveric kidney allograft. Spleen cells from cadaveric donors and peripheral blood lymphocytes from the respective recipients pretransplant (pre-Tx), at three and six months posttransplant (post-Tx) were obtained to perform one-way mixed lymphocyte cultures and flow cytometry analysis of lymphocyte subsets. The results indicated the development of donor-specific mixed lymphocyte culture (MLC) hyporesponsiveness as early as three months post-Tx, paralleled by a decrease in CD4+CD29+ helper-inducer cells and by an increase in CD8+CD45RA+ suppressor lymphocytes in peripheral blood. These changes were reflected in a very good clinical outcome of the patients. The present results further suggest that suppression of the immune system just before transplantation is a suitable method to induce early specific hyporesponsiveness to the allograft.

Adult↗