Zinc and aspergillus.
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Biomedical subjects
Publications and source records attributed to J Beck-Mannagetta.
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During the last years the appearance of radiopaque concrements in the maxillary sinus was reported. These could be identified as root-filling material for teeth of the upper jaw containing zinc oxide. This suggested that excess root-filling material containing zinc oxide in the maxillary sinus could favour the development of a local, non-invasive aspergillosis. Therefore, we tested Aspergillus fumigatus, A. flavus, A. terreus, A. nidulans and A. niveus for the influence of zinc oxide. Cza-pek-Dox agar was inoculated with the six different Aspergillus strains. Circular cavities stamped into the agar's center of each plate were filled with aliquots of the dilutions of a geometric series of zinc oxide dissolved in 0,1n H2SO4. All plates were then incubated for seven days at 37 degrees C. Readings were taken on the 3rd, the 5th and the 7th day. It became obvious that solved zinc oxide promotes the growth of all the tested Aspergillus species. This influence diminished with decreasing concentration. This effect could be observed on all days of measurement. Only A. niger showed an attenuated dependence, probably due to the a priori high growth rate of this species on Czapek-Dox agar. It is concluded that overfilling of maxillary teeth with a zinc oxide containing root filling material favours the development of local aspergillosis.
Repositioning of fractured fragments, stabilization with miniplates and reconstruction using autologous bone are commonly used techniques in the treatment of fractures of the orbital roof. Between 1988 and 1993, 101 patients were operated upon transcranially in the Department of Neurosurgery of the Landesnervenklinik Salzburg, for fractures of the orbital roof. The method, the timing and the results of treatments are presented. It can be demonstrated that even in severely injured patients the results were good in 79.2% (80 patients).
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A pathologic mandibular fracture in a 35-year-old woman 1 year after the attempted filling of a bone defect with particulate, dense hydroxyapatite resulted in a partial mandibular resection, allowing observation of cross sections of the mandible and associated soft tissue. Thin tissue sections were examined without decalcification. This permitted excellent observation of the hydroxyapatite particles, the bone, and the soft tissue. Osseointegration had occurred only in the areas closely associated with the bone. The immobility of the particles was a prerequisite for involvement with new bone formation. Hydroxyapatite particles in areas that allowed any mobility were surrounded by connective tissue with no bone formation evident. Heterogeneous particles were observed, indicating the possibility of a lack of purity in the hydroxyapatite ceramic, which may have contributed to the resorption of the particles. These findings, as well as other clinical and experimental findings, lead us to question the concept of hydroxyapatite as a bioactive ceramic that induces osteogenesis or osteoconductivity.
Antral aspergillosis has recently been found to be often induced by endodontic material (Endomethasone, N2, Riebler Material), that contains zinc oxide and paraformaldehyde. A study of 34 otherwise healthy patients with aspergillus infection of the maxillary sinus had a pathognomonic radiologic appearance noted in 26 patients (76%). All patients except one had a history of endodontic therapy. The radiology, pathogenesis, differential diagnosis, treatment, and prevention of solitary antral aspergillosis are briefly discussed.
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Recently the appearance of radiopaque 'concrements' in the maxillary sinus was reported. These radiodense objects could be identified as root-filling material for teeth of the upper jaw containing zinc oxide. This suggested that excess root-filling material containing zinc oxide in the maxillary sinus could favour the formation of a local, non-invasive aspergillosis. To verify this hypothesis in vitro, we tested the influence of zinc oxide on Aspergillus fumigatus, A. flavus, A. terreus, A. nidulans, A. niger and A. niveus. A geometric series of zinc oxide diluted in 0.1 N H2SO4 was used. Czapek-Dox agar was inoculated with the six Aspergillus species. Circular cavities stamped into the centre of each agar plate were filled with an equal amount of the dilutions and all plates were then incubated for 7 days at 37 degrees C. Readings were taken on days 3, 5 and 7. Soluble zinc oxide promoted the growth of all the tested Aspergillus species, the effect diminishing with decreasing concentration. This effect could be observed on all days of measurement. Only with A. niger was stimulation of growth minimal, probably because this species already exhibits a high growth rate on Czapek-Dox agar. Therefore, overfilling of maxillary teeth with a zinc oxide-containing root-filling material may favour the formation of local aspergillosis.
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