PubMed Health⌕ Search

Biomedical subjects

J Belcher

Publications and source records attributed to J Belcher.

26 records · Page 2Linked to original sources
PubMed1992

Effect of smoking on fibronectin production by human amnion and placenta.

Fibronectin production from amnion and placental tissues was evaluated in pregnant woman smokers and nonsmokers in order to examine if there were alterations of fibronectin metabolism in intrauterine tissues. In both amnion and placental tissues, cycloheximide inhibited the fibronectin output indicating that it was being synthesized. Mean fibronectin output by amnion in pregnant woman smokers was significantly lower than that in pregnant woman nonsmokers. In contrast, in the placenta from pregnant woman smokers, the output was significantly higher than that in pregnant woman nonsmokers. The present observations indicate that smoking alters an important biochemical constituent in amnion and placenta, possible leading to some complications.

Amnion↗
PubMed1992

Self-reported health care over the past 10 years: a survey of general practitioners.

To investigate how and where doctors receive their health care, 275 general practitioners were given a questionnaire about their health care in the previous 10 years; responses were received from 247 doctors (90%). Thirty nine per cent of the subjects were registered with a general practitioner who was independent of them. All but one of the remainder were registered with a practice partner, close friend or relative. Whatever the relationship of the subjects with their own general practitioner, personal health problems were managed to a great extent by themselves. Most (84%) of the medication taken in the previous five years had been self-prescribed and one third of medical investigations had been self-initiated. Over half of the general practitioners studied had seen a specialist about their health in the preceding 10 years; 51% had referred themselves. A 'jury' of seven general practitioners compared the subjects' referrals to a specialist with the care that would be expected for a non-general practitioner patient. Where the jury reached agreement, 68% of referrals were thought to have been appropriate; self-referrals were significantly more likely to be thought inappropriate (P less than 0.05); and self-treatment prior to self-referral to a specialist was considered inappropriate in 78% of cases. The amount of self-prescribed medication and frequency of consultation was the same, whatever relationship the subject held with the general practitioner. This study shows that most general practitioners manage their own health care. The question of whether this is always appropriate is raised and the provision of an occupational health service for general practitioners is discussed.

Humans↗
PubMed1989

Comparative effects of prazosin and propranolol on blood lipid profiles in hypertensive, hypercholesterolemic patients. Preliminary results.

Preliminary results are presented for the first 13 patients to complete an ongoing crossover study comparing the effects of prazosin and propranolol on blood pressure control, in which blood lipid profiles were monitored. The study population consisted predominantly of white men (mean age, 67.5 years) with mild-to-moderate hypertension and coexisting hypercholesterolemia (mean plasma cholesterol level, 244 mg/dl). Both agents resulted in statistically significant reductions in diastolic blood pressure. It is recognized that with prazosin use, there are generally no adverse changes noted between pre- and post-treatment lipid levels. The results of this study demonstrated a 243.5 mg/dl total cholesterol level at baseline and 229.5 mg/dl maintenance levels following prazosin treatment. In contrast, the results following propranolol treatment showed a tendency to increase plasma levels of triglycerides and very low-density lipoprotein cholesterol, and to reduce levels of high-density lipoprotein cholesterol and high-density lipoprotein2 cholesterol. Because of a great deal of variability in lipid responsiveness to the study drug, none of the above changes attained statistical significance. Additional data from this ongoing study are required to confirm these preliminary observations.

Adult↗
PubMed1988

Relationship between the deinstitutionalization model, psychiatric disability, and homelessness.

A follow-up study that tracked a sample of 133 people released from a state mental hospital showed that 35 percent became homeless within three months, supporting the theory that deinstitutionalization contributes to homelessness. An in-depth, qualitative tracking procedure provides data to describe key characteristics of the homeless person and the process by which people become homeless. These data point to solutions for improved care of severely mentally disabled people for whom the aftercare system has failed.

Adult↗
PubMed1985

Identification and characterization of ATP-dependent proton transport by rat liver multivesicular bodies.

Multivesicular bodies (MVB), prelysosomal organelles in the endocytic pathway, were prepared from estrogen-treated rat livers and examined for the presence of ATP-dependent proton transport. Vesicle acidification, assessed by acridine orange fluorescence quenching, was ATP dependent (ATP much greater than GTP, UTP), was enriched 25-fold over homogenate, was abolished by pretreatment with protonophores or a nonionic detergent, exhibited a pH optimum of 7.5, was inhibited by N-ethylmaleimide (NEM) (IC50 approximately 5 microM) and N,N'-dicyclohexylcarbodiimide (IC50 approximately 5 microM), and was resistant to inhibition by vanadate, ouabain, and oligomycin. Acidification exhibited no specific cation requirement; however, maximal rates of acidification depended upon the presence of Cl- (Km approximately 20 mM). Other anions were less effective in supporting acidification (Cl- greater than Br- greater than much greater than gluconate, NO-3, SO2-4, and mannitol), and indeed NO-3 inhibited acidification even in the presence of 150 mM Cl-. The proton transport mechanism appeared to be electrogenic based on: (a) enhancement of acidification by valinomycin in the presence of K gluconate, and (b) ATP-dependent fluorescence quenching of bis(3-phenyl-5-oxoisoxasol-4-yl)pentamethine oxonol, a membrane potential-sensitive anionic dye. Furthermore, the magnitude of the pH and electrical gradients generated by the proton transport mechanism appeared to vary inversely in the presence and absence of Cl-. Finally, MVB exhibited ATPase activity that was resistant to ouabain and oligomycin, but was inhibited 32.3% by 1 mM NEM, 33.7% by 200 microM dicyclohexylcarbodiimide, and 18.7% by KNO3. In isolated MVB, therefore, the NEM-sensitive ATPase activity may represent the enzymatic equivalent of a proton pump. These studies identify and characterize an ATP-dependent electrogenic proton transport process in rat liver MVB which shares many of the properties of the proton pump described in clathrin-coated vesicles, endosomes, lysosomes, Golgi, and endoplasmic reticulum from liver and other tissues. Acidification of MVB differed somewhat from that of rat liver clathrin-coated vesicles in response to Br- and NO-3, suggesting that membrane properties of these two organelles might differ.

Acridine Orange↗
PubMedDate not supplied

Rural elderly present need for nursing continuity.

Over 25% of the elderly reside in rural areas. Elders residing in rural areas have incomes that are approximately 20% less than their urban counterparts. One year of data on the 1,972 admissions of Medicare patients to a 150-bed rural hospital was studied to seek the commonalties marking four LOS categories of patients, including their 17+ day LOS outliers. Sixteen percent of all the Medicare admissions who tended to be older, have chronic illnesses and needed multiple services, fell into the 17+ day LOS group where reimbursement covered only one-third of the actual cost of care provided. ICD-9 codes associated with the cardiac diagnostic category dominated, with 21.5% of those included in this resource-intensive group. In preparing for capitated reimbursement, all efforts to develop systems to decrease ED visits and hospital stays, while enhancing quality of life for the chronically ill, must be implemented.

Aged↗