The interaction of a newly designed benzocycloheptaindol-6-one derivative with DNA has been investigated by complementary spectroscopic techniques including absorption, circular and linear dichroism. Footprinting measurements were performed to delineate the sequence-selectivity of the drug-DNA interaction and a plasmid relaxation assay was used to study the effects of the drug on human DNA topoisomerases I and II. The results clearly indicated that the test compound behaves as a typical DNA intercalating agent but does not stimulate DNA cleavage by topoisomerases. At the cellular level, the cytometry measurements showed that the drug provoked a marked accumulation of HL60 human leukemia cells in the G2/M phase of the cell cycle. DNA is thus identified as a valid target for this new series of drugs particularly toxic to human (HL60) and murine (P388) leukemia cells.

Animals↗

PubMedDate not supplied

[Chronic disseminated histiocytosis in the adult. Apropos of a case with a 12-year remission].

The authors report a rare case of diffuse histiocytosis X in an adult aged 47 followed up for 12 years. There was extensive skeletal and oral dissemination but without visceral, cutaneous or neurological involvement. Blood investigations showed only a raised erythrocyte sedimentation rate. The literature of histiocytosis is reviewed; few papers could be found concerning this type of disease in adults.

Chronic Disease↗

PubMedDate not supplied

[Failures and limitations of the Papineau technic. A report of 54 cases].

The authors have treated 41 cases by Papineau's technique. There were 19 cases of osteitis, and 35 septic non-unions (9 in the femur, 16 in the tibia and 10 in other sites). Twenty-four cases healed, the average follow-up being one year. The causes of failure were analysed. Some are related to the technique itself - sepsis and fractures at the sites of graft removal, inadequate quantities of cancellous bon, long delay in bone union and skin problems particularly in the leg. The indications for this technique are compared with those of other techniques. It is concluded that it is a useful technique; only two patients required amputation.

Adult↗

PubMedDate not supplied

[Spinal chordoma. A case report on chordoma of the L3 vertebra].

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Biopsy↗

PubMedDate not supplied

[Psycho-pathological approach to suicide attempts by ingestion of "Destop"].

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Burns, Chemical↗

PubMedDate not supplied

[Anesthesia and resuscitation in surgery for aseptic osteonecrosis in patients with renal transplants].

Aseptic osteonecrosis following renal transplantation occurs in about 20 p. 100 of cases and entails important functional consequences. 16 patients with kidney transplant and 4 patients on chronic dialysis because of previous graft rejection underwent 27 orthopaedic operations, 8 of which being hip arthroplasties. During and after the procedure the anesthesiologist had to manage with drug interaction, coagulation disorders, blood compatibility problems etc... Despite poor general condition of patients and the risk of infectious complications linked to the never interrupted immunosupressive treatment, the surgical results of our serial were good.

Adolescent↗