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Biomedical subjects

J Bevenius

Publications and source records attributed to J Bevenius.

12 recordsLinked to original sources

Conservative management of erosion-abrasion: a system for the general practitioner.

This final article in a series of three covering general practice aspects of erosion-abrasion presents a three-phase system for clinical management of erosive-abrasive damage. The first phase comprises pre-restorative case evaluation, initial measures to control factors predisposing to continued erosion-abrasion and where necessary the provision of acrylic overlays to restore facial height. In the second phase the teeth are systematically restored using an acid-etch composite resin technique; guidance for comfortable occlusion is provided by the patient acceptance method. The third phase comprises follow-up, including monitoring of salivary and bacteriological parameters, maintenance of the restorations and modifications of preventive measures necessary to preserve the dentition restored by multiple composite therapy. Modern oral physiological concepts and recent developments in multiple composite therapy and erosion control are discussed.

Composite Resins↗

The micromorphology in vivo of the buccocervical region of premolar teeth in young adults. A replica study by scanning electron microscopy.

The buccal surfaces of premolar teeth are common sites of gingival recession, generally attributed to overzealous oral hygiene. Scanning electron microscopy (SEM) of replicas made from dental impressions was applied to document the micromorphology of the buccocervical region of all premolar teeth in 27 dentally healthy, young adults. The SEM observations were correlated with clinical examination. Of the 216 sites, one-third, predominantly the maxillary first premolars, had gingival recession, but fewer than 50% were clinically discernible. The exposed roots were devoid of cementum, and the dentinal surface was smear-like or dotted with tubular apertures from which droplets of fluid extruded. SEM of replicas of gingiva, recorded as clinically healthy, frequently showed signs of inflammation: fluid exudate and distortion of gingival contour by swelling. The cervical enamel of healthy and affected sites showed characteristic periodic fissure-like cracks, probably enamel tufts. The high frequency of subclinical gingival recession, exposed cervical dentin, and gingival inflammation in dentally healthy young adults, in the absence of abrasion of hard or soft tissues, indicates the need to review conventional concepts of initiation of buccal recession and root exposure.

Adult↗

Site-related streptococcal attachment to buccocervical tooth surfaces. A correlative micromorphologic and microbiologic study.

Scanning electron (SEM) microscopy of epoxy replicas made from dental impressions has shown that in buccal gingival recession the root surfaces are devoid of cementum, leaving the dentin exposed. In this study replication techniques were applied to correlate the micromorphology of the buccocervical region with early streptococcal attachment. The subjects were 27 healthy young adults. The buccocervical surfaces of all the premolars were meticulously cleaned. The subjects fasted for 2 h before impression-taking. Replicas were made from impressions in hydrophilic A-silicone, and streptococcal attachment was visualized by light microscopy of mitis-salivarius agar replicas incubated anaerobically for 48 h. The surface micromorphology was documented by SEM of corresponding epoxy replicas. Colonization only 2 h after cleaning was very sparse. Sites with healthy or inflamed gingivae had markedly different colonization patterns in the sulcular region. In 4 subjects with a total of 12 sites where gingival recession, undetected clinically, was disclosed by SEM, representative colonies were retrieved and identified microbiologically to species level. Two healthy sites per subject were also sampled. Streptococcus mutans and S. sobrinus were identified from eight sites with exposed root dentin. S. oralis predominated on the enamel surfaces. The method offers a valuable complement to in situ and in vitro microbiologic studies of exposed dentin and a novel technique for sampling clinical isolates of streptococci.

Adolescent↗

Idiopathic cervical lesions: in vivo investigation by oral microendoscopy and scanning electron microscopy. A pilot study.

The development and progression of cervical lesions have not been thoroughly documented. Clinically, two morphologically distinct forms, saucer-or wedge-shaped, have been described. In this study, the micromorphology of manifest cervical lesions was documented in six subjects, by SEM replication and microendoscopy. Optoelectronic recordings were made of extreme mandibular lateral excursions. Salivary parameters were evaluated and a detailed case history, including oral hygiene and dietary habits, was taken. Lesions of varying severity could be observed in the same subject. Longitudinal fractures of the enamel were common. The dietary analyses were uneventful and salivary values were normal. No correlation was found between brushing habits and the location and severity of the lesions. The optoelectronic recordings, however, indicated a possible correlation between irregular lateral excursion and the severity and location of the lesions, with a tendency for fewer and less severe lesions on the preferred chewing side. The results have indicated some areas of interest for future studies applying non-invasive observation methods.

Adult↗

The amelocemental junction in young premolar teeth. A replica study by scanning electron microscopy.

To investigate the validity of traditional data on the frequency of various relationships of root cementum to coronal enamel at the amelocemental junction, the relationship in 50 young premolar teeth extracted on orthodontic indications was documented, using replica techniques for scanning electron microscopy (SEM), combined with polarization microscopy of thin ground sections (< 40 microns). Of 200 surfaces, 30 were excluded because of damage during specimen preparation. Replicas of the other 170 surfaces were photographed at 20x and 80x in SEM and classified as having an edge-to-edge relationship of enamel to cementum in 76% and an overlap of cementum onto the cervical enamel in 14%. What appeared to be a gap between enamel and cementum at low magnification (10%) was seen at high magnification to be a ditch or trough in the cementum: no gaps with exposed dentin were observed in SEM. Polarization microscopy of ground sections of the original specimens confirmed the SEM classification. In this material, the edge-to-edge relationship of enamel to cementum predominated: overlapping of cementum onto the enamel was less prevalent than previously reported. The methods applied in this study circumvented some major sources of artefact formation due to shrinkage during specimen preparation, which may previously have caused misinterpretation of 'gap' relationships.

Bicuspid↗

In vitro and in vivo replication for scanning electron microscopy of the cervical region of human teeth.

A replica technique for scanning electron microscopy (SEM) of the cervical region of human teeth was evaluated on extracted premolar teeth by comparing the replicas and the original specimens in the SEM. For in vivo application, the technique was modified to circumvent contamination by saliva and gingival exudate. Impressions were taken with an addition silicone polyvinylsiloxane material and the replicas were poured in epoxy resin die material. A surface active dentine conditioner facilitated flow of the impression material into irregular surface areas; in vivo a scavenger impression was used to remove surface debris. Custom trays were made of light-cured acrylic resin for the in vivo impressions. The method faithfully reproduced surface detail in the amelocemental region. In vivo the scavenger impression followed by application of the surface-active conditioner effectively cleaned the tooth surface. The custom tray allowed selection and inclusion of landmarks and ensured reproducibility. The method meets the requirements of a simple, reproducible, non-invasive means of documenting the micromorphology of the cervical region of the teeth.

Bicuspid↗

Chairside evaluation of salivary parameters in patients with tooth surface loss: a pilot study.

In cases of tooth surface loss, the possibility of an erosive component should be considered, even when the lesions have the characteristic clinical appearance of abrasion or attrition. Individual susceptibility or resistance to erosive damage has been attributed to variations in the quality of saliva. Two recent studies have indicated that rate of flow of saliva and buffer capacity may be important determinants in erosion susceptibility. These parameters may be readily assessed using the chairside kits developed in Scandinavia for assessing caries susceptibility. In eight consecutive patients referred to the University of Queensland Dental School for investigation of tooth surface loss, six had no measurable quantities of resting whole saliva, four had low values for stimulated saliva flow rates, and only two patients had buffer capacities within the normal range. The results indicate the need for a larger scale study of salivary parameters in this patient group. The chairside kits provide a simple, rapid, hygienic means of assessing salivary values.

Adolescent↗

Intraoral application of microcolpohysteroscopy. A new technique for clinical examination of oral tissues at high magnification.

Colposcopy is an established technique for diagnosis in gynecology. A recently developed optical instrument is the contact microcolpohysteroscope for macroscopic examination of the cervical mucosa. This article evaluates the application of the contact microcolpohysteroscope for examination and recording of the surface topography of the hard and soft tissues of the oral cavity. This is a promising, noninvasive method of observation of tissue surfaces at high magnification. Modifications of the method for oral use are described, and potential applications for diagnosis and research in dentistry are proposed.

Colposcopes↗

Caries risk in patients with Crohn's disease: a pilot study.

Crohn's disease is a chronic inflammatory bowel disease of unknown cause with unpredictable remissions and exacerbations. Associated nutritional deficiencies include those involving zinc, magnesium, vitamin B12, folic acid, and vitamin D. A group of patients with Crohn's disease underwent detailed cariologic investigation at the Department of Cariology, Karolinska Institutet, Stockholm. Factors predisposing to caries were evaluated according to Krasse's concept of caries risk. On this basis, 11 of the 15 patients had a high caries risk. The concept of caries risk acknowledges the multifactorial background of caries initiation and progression and, in this pilot study, has proved to be an appropriate basis for evaluation of patients with chronic disease. Guidelines for preventive programs appropriate for patients with Crohn's disease, based on the findings of this study, are presented.

Adolescent↗