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J Biewenga

Publications and source records attributed to J Biewenga.

61 records · Page 4Linked to original sources
PubMed1975

LDH-IgA immunoglobulin complexes in human serum.

Human sera containing complexes of lactatedehydrogenase (LDH) and immunoglobulin A (IgA) were studied. Such complexes could be demonstrated in a number of sera with abnormalities in electrophoretic pattern of the LDH isoenzymes. LDH-IgA complexes were observed in a number of sera in which the LDH-2 band was missing, in a serum with all LDH activity in the betta-gamma-globulin region, but also in some sera with only minor abnormalities in the LDH isoenzyme pattern. It is shown that the IgA bound to LDH is of the kappa light chain type. Auto-bodies were not detected in the sera. Therefore, the formation of the LDH-IgA complexes does not seem to be the result of an antigen-antibody reaction.

Adolescent↗
PubMedDate not supplied

Anti-TNP-forming cells in rats after different routes of priming with TNP-LPS followed by intranasal boosting with the same antigen.

To study the reactivity of nasal-associated lymphoid tissue (NALT) and its position in the mucosal immune system, rats were intranasally challenged with 200 micrograms TNP-LPS. Priming had occurred 15 days previous to the challenge with the same antigen and dose, either intranasally, intratracheally, subcutaneously in the cheek or intraperitoneally. The number of anti-TNP antibody-forming cells (AFC) was determined in various tissues using the conjugate TNP/alkaline phosphatase. Generally, anti-TNP AFC were predominantly found in the posterior cervical lymph nodes, while NALT contained hardly any such AFC. The highest response in the posterior cervical lymph nodes occurred on day 5, after subcutaneous priming and intranasal boosting. This also evoked peak responses in several other tissues. The highest response in spleen and lung occurred on day 7 after intraperitoneal priming and intranasal boosting. Irrespective of the immunization route, IgA was the least produced isotype in the spleen as compared to antigen-specific IgG and IgM. In the posterior cervical lymph nodes, besides specific IgG and IgM, a considerable proportion of specific IgA was produced. All four immunization routes yielded anti-TNP antibodies in serum. As for the non-lymphoid cells, the intratracheal-intranasal immunization protocol induced an increase in pulmonary macrophages on days 3 and 5. The immunological role of lung macrophages is discussed.

Administration, Intranasal↗
PubMedDate not supplied

Ontogeny of reticulum cells in the rat intestine and their possible role in the development of the lymphoid microenvironment.

Ontogeny of reticulum cells (RC) in the rat intestine in relation to the development of the gut-associated lymphoid tissue (GALT) was studied using a panel of monoclonal antibodies (mab) directed against RC in peripheral lymphoid organs, ED10-ED15. The mab ED10 specific for RC in the spleen T cell area, recognized an epitope on gut RC, which cells seem to be involved in the influx and accumulation of lymphocytes in the lamina propria and in Peyer's patches (PP) and proximal colonic lymphoid tissue (PCLT). The mab ED11 which recognizes RC in the T cell area and B cell follicles of spleen, stained follicular dendritic cells (FEC) in the B cell area of the mesenteric lymph node (MLN), PP and PCLT. The ED11 expression occurs earlier and reveals stronger staining in MLN as compared to those in PP and PCLT, suggesting the prominent role of MLN in the generation and proliferation of B cells in the gut mucosal immune system. The mab ED13 specific for RC in the B cell area of the lymph nodes, stained the basement membrane of the epithelium overlying PP and PCLT, and high endothelial venules (HEV), suggesting that this might be involved in providing the microenvironment for the development and differentiation of follicle-associated epithelium, and facilitating lymphocyte traffic. The mab ED12 specific for RC in the paracortex of peripheral lymph nodes, gave a diffuse nonspecific staining in the gut, whereas mab ED14 and ED15 are markers for common connective tissue cells. We conclude that the gut RC are morphologically and phenotypically heterogenous. ED10+, ED11+, and ED13+ RC are probably involved in the development of the gut lymphoid microenvironment.

Aging↗