PubMed Health⌕ Search

Biomedical subjects

J Bohn

Publications and source records attributed to J Bohn.

25 records · Page 2Linked to original sources
PubMed1982

Effects of low concentrations of antibiotics on Escherichia coli adhesion.

We have previously shown that subinhibitory concentrations of antibiotics may influence the adhesion of Escherichia coli SS142 to human epithelioid tissue culture cells. This report shows that these effects are not limited to E. coli SS142 or to our tissue culture system. Most of the 10 E. coli strains studied showed decreased adhesion to Intestine 407 tissue culture cells after growth in 25% of the minimum inhibitory concentration of streptomycin, tetracycline, trimethoprimsulfametrole, chloramphenicol, and clindamycin. Nalidixic acid at 25% of the minimum inhibitory concentration caused an increase of adhesion. The hemagglutinating activity of the five hemagglutinating strains and the adhesiveness of E. coli SS142 to human buccal cells were similarly affected by low concentrations of the above-mentioned antibiotics. We conclude that E. coli adhesion to human epithelioid tissue culture cells is a valid model of bacterial adhesion because of its high accuracy and reproducibility.

Anti-Bacterial Agents↗
PubMed1981

[End-diastolic volumes of the left ventricle in computer tomography in comparison to heart catheter ventriculography].

In 47 patients the authors calculated the volume at the end of a diastole according to both the cardiac catheter ventriculogram and the CT ventriculogram, comparing the results obtained with each of these methods. A linear regression was found. The correlation coefficient was approximately r = 0,96; n = 47. Cardiological examination revealed that of the examined patients (including the cardiac catheter finding) 18 patients had coronary heart disease, whereas 9 had cardiomyopathy, 6 arterial hypertension, 9 had various cardiac abnormalities and 5 did not show any organically manifest heart disease. The article discusses CT determination of the volume at the end of the ventricular diastole, and discusses the results.

Cardiac Catheterization↗
PubMed1981

[Optimized calculation of left ventricular enddiastolic volume by equilibrium radionuclide ventriculography (MUGA)--the influence of background correction and absorption (author's transl)].

Left ventricular (LV) enddiastolic volume (EDV) was calculated in 23 patients from background-corrected enddiastolic count rates (ROI technique). The calculation in volume units (ml) was based on the measurement of a well-defined amount of patients' venous blood (50 ml in a syringe) counted by the gamma camera. Decrease in count rate due to tissue attenuation was corrected for by calculation of soft-tissue absorption in the thoracic and LV wall as well as the blood self-absorption within the LV, applying a rotational ellipsoid with an assumed LV depth of 1.5 times that of the LV width within the LV ROI. Furthermore, the influence of various models of LV background superimposition (homogeneous, parabolic and no background) were tested correlatively for calculations of volume. Best correlation with cineventriculographically measured volumes (r = 0.92) was found with a parabolically weighed background correction, determining an individual value of background superimposition for each LV pixel. This approach was chosen because a homogeneous superimposition is an inadequate model to correct for background due to the geometric extension of the LV. Volumes, parabolically corrected were found to correlate most closely to cineventriculography (CVG) (VMUGA = 0.997 X VCVG - 12.6 ml).

Cardiac Volume↗
PubMedDate not supplied

Human monoclonal IgM antibodies from foetal B-cell hybridomas directed against a surface antigen on human tumour cells.

In order to assess the existence of B lymphocytes capable of producing anti-tumour antibodies in non-tumour-bearing individuals, human lymphocytes derived from foetuses and adults were fused with the heteromyeloma cell line CB-F7. By indirect immunofluorescence, 29 out of 4,472 IgM-producing hybridomas (from 8 foetuses and 8 adults) were shown to produce antibodies which bind to colon carcinoma lines Colo205 and SW620, Raji lymphoma cells and small cell carcinoma of the lung. In vitro growth of tumour cells recognized by these antibodies was inhibited. The antibodies also mediated complement-dependent cytotoxicity. All antibodies tested recognized a cell surface molecule of 55 kDa. Southern blot hybridization analysis of hybridoma DNA with a human JH probe showed that the hybridomas were derived from clonally unrelated B cells. These results demonstrate that human foetal and adult B cells from non-tumour-bearing individuals are able to produce IgM antibodies recognizing defined cell surface molecules expressed on some tumour cells.

Adult↗
PubMedDate not supplied

[Mental gait training -- effectiveness of a therapy method in the rehabilitation after hip-replacement].

PURPOSE: New neurophysiological findings cover the effectiveness of movement images to the movement learning and deliver the theoretical base for the reasonable application of mental training forms in orthopaedic rehabilitation. In support of the mental training set-up in the high-performance sport, suitable movement images are developed and trained systematically in the mental gait training. The present study examines the question of the effectiveness of mental gait training. METHOD: A randomised control group study [experimental group: mental walking training; control group: no treatment (treatment additional to the customary physiotherapy)] with measuring repetition was carried out. With 24 patients after total hip replacement surgery the relevant kinematic data were collected by applying the procedure SIMI Motion to the walking movement. RESULTS: The experimental group improved in comparison to the control group in the variable stride length, as well as in the factor variable kinematics significantly. CONCLUSION: It can be stated that this study gives further proof for the reasonable application of mental training forms in rehabilitation. Further studies on the application of mental training forms to the movement learning (concerning different movements and further syndromes) must follow to provide further support of the effective and efficient application of these procedures within the context of rehabilitation.

Adult↗