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J Botella

Publications and source records attributed to J Botella.

42 records · Page 3Linked to original sources
PubMedDate not supplied

Hemodiafiltration without replacement fluid. An experimental study.

Paired filtration dialysis (PFD) is the only hemodiafiltration (HDF) technique in which the ultrafiltrate is continuously available but not mixed with the dialysate. As is the case during all convective or predominantly convective techniques, use of a replacement fluid is necessary in an amount equal to the difference between the ultrafiltrate and the desired patient weight loss. This replacement fluid must have an adequate electrolytic composition (Na+, Ca++, and buffer), and must be sterile and pyrogen free. Using an uncoated adsorbent charcoal cartridge (130 g), the ultrafiltrate obtained in PFD was regenerated, eliminating both the small (except for urea, glucose, and phosphates) and medium-to-large solutes but not the electrolytes and bicarbonate. This verified the ultrafiltrate's possible use as replacement fluid. This technique experimentally studied during 24 standard PFD sessions, with a total mean ultrafiltrate of 9,950 +/- 860 ml, allowed a replacement solution to be obtained with the following mean +/- SD composition: pH 7.467 +/- 0.122, HCO3- 27.0 +/- 2.12 mmol/L, Na+ 137.4 +/- 2.6 mmol/L, K+ 4.1 +/- 0.83 mmol/L, Ca++ 1.12 +/- 0.19 mmol/L, urea 68.3 +/- 16.2 mg/dl, creatinine 0.08 +/- 0.02 mg/dl, uric acid 0.05 mg/dl, phosphates 2.77 +/- 0.71 mg/dl, beta-2 microglobulin 0.5 +/- 0.4 mg/L, and atrial natriuretic peptide 4.41 +/- 5.6 pg/ml.(ABSTRACT TRUNCATED AT 250 WORDS)

Charcoal↗
PubMedDate not supplied

Continuous pH and HCO3 monitoring during hemodiafiltration without blood sampling.

The correction of acid-base balance during hemodialysis, especially in high-efficiency techniques, could present some problem related to the lack of an adequate monitoring of pH and blood gases. During hemodiafiltration (HDF), performed with the two-chamber technique (paired filtration dialysis, PFD), the ultrafiltrate (Uf) is continuously available, unmixed with the dialysate. Connecting a pH electrode (as Ag/C1Ag) to the Uf circuit, the authors made 40 determinations on 16 different PFD patients, and they correlated the Uf values obtained with those measured on arterial blood with standard methods. The one sample analysis gave a t = 10.145 (p = 0.0), and the linear regression analysis an r = 0.931 (p = 0.0). At 30 min, in 8 PFD patients, the HCO3 values obtained from Uf, pH and transcutaneous PCO2, gave a t = 6.37 (p = 0.0004), and an r = 0.939 (p = 0.00052). In conclusion, during HDF performed with PFD, continuous pH monitoring of the patient is possible without blood sampling. Moreover, correlation with the transcutaneous PCO2 measurement could provide HCO3 values in real time.

Acid-Base Equilibrium↗
PubMedDate not supplied

Interaction of new 19 nor progesterone derivatives with progestagen, mineralocorticoid and glucocorticoid cytosolic receptors.

Structure-activity relationships were studied in vitro on a number of natural and artificial steroids in order to assess their progestagen specificity. These substances included a new compound derived from 19 nor progesterone, TX066 or nomegestrol acetate, and two synthetic intermediates, TX045 and TX071, all prepared by Laboratoires Theramex. The experiments involved binding competition on the cytosolic receptors prepared from target organs against specific radiolabelled ligands. Relative affinities were determined in rats against progesterone (P), aldosterone (A) and dexamethasone (DM), by displacement of: (3H)-ORG 2058 from the progestagen receptor of uterus, (3H)-A from Type I (mineralocorticoid) receptor of the kidneys and (3H)-DM from glucocorticoid receptors of the kidney (Type II) and of the liver. The various modifications introduced in the progesterone molecule led to sequences in competition potency which were either parallel or opposite in the progesterone compared to the 19 nor progesterone series. The main practical conclusion is that TX066 which is intended for use as a progestagen presents very little mineralo- and glucocorticoid activities at the receptor level, while its affinity for the progestin receptor is nearly as good as that of progesterone. The two derivatives TX045 and TX071 displayed very little or no progestagen affinity while their mineralo- and glucocorticoid potencies were between those of 19NP and TX066.

Animals↗