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J Brouwer

Publications and source records attributed to J Brouwer.

114 records · Page 7Linked to original sources
PubMed1975

Experience with the use of polyglycolic acid suture material (Dexon) in 500 surgical patients.

A highly concise review is presented of the experimental and clinical literature concerning polyglycolic acid suture material, marketed for clinical use for the first time in 1970. It is a synthetic, completely absorbable, non-toxic product, which not only offers a number of advantages over catgut, but may also be used where non-absorbable materials were formerly applied exclusively. Our favorable experiences in 500 patients, who were subjected to many different kinds of general surgical operations, are in agreement with those published in the literature. We have gained the impression that suture fistulization, which may lead to protracted morbidity after use of non-absorbable material, has decreased in incidence and severity after the use of PGA suture material. This is a definite advantage.

Humans↗
PubMedDate not supplied

DNA insertions in the 'silent' regions of the 2 microns plasmid of Saccharomyces cerevisiae influence plasmid stability.

The 2 microns plasmid of the yeast Saccharomyces cerevisiae is in principle a suitable vector for expression of foreign genes, due to its high copy number and extreme stability. However, the cloning of genes into 2 microns often results in a reduced copy number and/or reduced stability. One reason for this observed instability could be that the inserts in general were made in one of the several open reading frames (ORFs) of the plasmid. Therefore we studied the effect on stability of insertions in the silent regions of 2 microns without interrupting any known essential regions or ORFs. Using the SnaBI site, a yeast-integrating plasmid (Yip5) was introduced into the region between the ARS and STB locus in two possible orientations. The resulting plasmids could be stably maintained in the cells without the need for complementation by the wild-type 2 microns plasmid. However, the stability of these plasmids in a cir. host was still one to two orders of magnitude lower (0.2% and 0.8% respectively) as reported for the wild-type 2 microns (0.01%). Removal of 2 kb of the bacterial sequences from Yip5 did not increase stability. The stability was dependent on the orientation of the insert. We found that in the less stable orientation, transcription originating from the insert was running into the STB region. DNA inserted in the XmaIII site located outside the ORFs in the REP2/FLP intergenic region influenced both stability and copy number of the plasmid. These effects are strongly dependent on the size of the insert. Insertion of a 2 kb DNA fragment increased the copy number, probably through an effect on FLP expression.

Blotting, Northern↗