Measuring coronal biparietal diameter.
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Female↗
Biomedical subjects
J Bukovsky
Publications and source records attributed to J Bukovsky.
Identification and characterization of the NMYC gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities.
Increased N-myc (now designated NMYC in human gene nomenclature) gene expression has been detected at the transcriptional level in certain types of neoplasms. As yet, the N-myc gene product has not been identified. To detect and characterize the N-myc gene product, we have developed monoclonal antibodies against the putative N-myc gene product made in Escherichia coli as a fusion protein. The antibodies that recognize the N-myc-specific regions were selected on the basis of their reactivities to different portions of the fusion protein. These monoclonal antibodies detect a pair of closely migrating polypeptides of 60 and 63 kDa in nuclear fractions of human neuroblastoma cells. The relative levels of the polypeptides are roughly proportional to the level of N-myc transcripts present in a panel of neuroblastoma lines. These two polypeptides have a half-life of approximately equal to 35 min, and they are indistinguishable from each other by their epitopic profiles.
Antibodies, Monoclonal↗
Production, purification, and characterization of excitability-inducing molecule.
Excitability-inducing molecule (EIM) is a high molecular weight polymeric protein. It is a channel-forming ionophore which can induce action potential in lipid bilayers and lyse red blood cells. It is also a potent mitogen for mouse B lymphocytes. EIM is produced and secreted into a chemically defined medium during the early growth period by Enterobacter cloacae ATCC 961. It is now 6000-fold purified as compared to the original egg white EIM. Production of the active material requires calcium ion. EIM contains 10 to 20% lipid by weight which is primarily composed of fatty acids, with an unsaturated 18-carbon chain. Amino acid analysis shows 18 common amino acids. The maximum specific activity of 18 common amino acids. The maximum specific activity of EIM is associated with the molecular weight of 3.6 x 10(5).
Action Potentials↗