Biomedical subjects
Publications and source records attributed to J Burian.
The authors tested the possibility to use the method of colony hybridization for screening of thermolabile haemolysin (phospholipase C) in strains of Pseudomonas aeruginosa. As a radioactively labelled probe they used SmaI fragments of the pGV26 plasmid, which contains the 6.3 kb fragment of the chromosome of strains P. aeruginosa PAD1 with the gene for phospholipase C.
The multiplasmid clinical E. coli KL4 strain isolated from urine of a patient was examined for resistance to antibacterial substances and the number of plasmids. The distribution of resistance genes to antibiotics in different plasmids was assessed. All assessed resistances were transmitted by the mechanism of bacterial conjugation. By means of conjugation of the clinical E. coli strain KL4 and E. coli DH1, transformation of the DH1 strain by plasmid DNA and electrophoretic analysis two R plasmids were identified. The largest plasmid carries the gene for resistance against tetracycline and it is conjugative. Another three plasmids were mobilized by this plasmid during conjugation. The largest among them codes resistance against ampicillin, azlocillin and ticarcillin. From the total of five plasmids of the KL4 strain three were cryptic with regard to the resistance phenotype.
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In coronary bypass surgery myocardial protection by injection of a cardioplegic fluid is made uneven by the presence of stenotic segments. In order to make it even, the authors suggest that the fluid should be injected first into the aortic origin, then into the coronary sinus. This method should provide greater safety when multiples bypasses are performed and in cases of impaired left ventricular function.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.