Biomedical subjects
Publications and source records attributed to J Butterworth.
Cultured amniotic fluid cells had a higher level of alpha-L-fucosidase than skin fibroblasts. The early passages of amniotic fluid cells had a higher activity than later passages, whilst skin fibroblast activity was unrelated to passage. For both cell types alpha-L-fucosidase activity fell on subculture, rising again at confluency.
Total and % A hexosaminidase were similar for primary cultures and later passages of amniotic fluid cells. The culture variables-through serial passage, within a passage and replicate primary cultures-resulted in some variation in total hexosaminidase, but an insignificant change in % A hexosaminidase. They are unlikely to give rise to any problems in the antenatal diagnosis of Sandhoff's and Tay-Sachs diseases.
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