Biomedical subjects

J Bux

Publications and source records attributed to J Bux.

59 records · Page 4Linked to original sources

PubMed1990

[Serologic characterization of granulocyte-specific antibodies in neonatal alloimmune neutropenias].

Serological properties of nine granulocyte specific alloantibodies in maternal sera and sera of the newborns were investigated by granulocyte immunofluorescence, granulocyte agglutination and granulocyte cytotoxicity. In the immunofluorescence assay the reactions were more prominent than in the agglutination assay. Only one antibody yielded a positive reaction in the cytotoxicity assay. Four NA2-, two NA1- and one NB1-antibody could be identified. The immunofluorescence assay showed a mixed pattern with the NB1-antibody, indicating a heterogenous distribution of the NB1-antigen on granulocytes of some donors. In titration studies with heterozygous and homozygous donors a gene-doses-effect was demonstrable.

Agglutination Tests↗

PubMed1990

[Preparation of leukocyte-poor thrombocyte concentrates from buffy coat by using a new quadruple bag system].

It has repeatedly been shown that platelet concentrates (PC) can be prepared from buffy coat in guadruple Saline(S)-Adenin(A)-Glucose(G)-Mannitol(M) (SAG-M) systems. Their in vivo and in vitro quality was reported to be excellent and their contamination with leucocytes was 10-20 times lower than in PC prepared from platelet rich plasma. We have confirmed these observations (unpublished results). One particular technical problem which we encountered was the appearance of folds in the satellite bag during the second centrifugation step rendering the purity of the PC quite unpredictable. In collaboration with Biotrans, we have developed a modified quadruple bag (733861100) including a special insert (Heraeus Christ, Osterode, FRG) for the centrifugation rotor which has proved advantageous over existing systems and will be briefly described.

Blood Component Removal↗

PubMed1990

[Quality of thrombocyte preparations from buffy coat or platelet-rich plasma].

One hundred platelet concentrates, fifty prepared from buffy coat and fifty prepared from platelet-rich plasma were examined. In platelet concentrates from buffy coat a lower leukocyte contamination (1.4 +/- 1.2 x 10(7) vs 13.2 +/- 15 x 10(7], a significant higher ATP content (6.3 +/- 1.7 mmol/10(14) platelets vs 3.9 +/- 1.9 mmol/10(14) platelets) and a better response in ADP- or collagen-induced aggregation could be determined during storage of five days. Our results indicate that preparation of platelet concentrates from buffy coat is preferable to their preparation from platelet-rich plasma.

Adenosine Triphosphate↗

PubMed1989

[Autoimmune neutropenia: clinical aspects, diagnosis and therapy].

Autoimmune neutropenia is a rare immunohematological disorder occurring as an idiopathic as well as a symptomatic variety associated with different diseases. Autoimmune neutropenia has previously been reported predominantly in adults, but is now found more frequently in young children. The neutropenia usually leads to recurrent mild bacterial infections. Apart from blood count and bone marrow evaluation assessment of granulocyte-specific antibodies is essential for establishing the diagnosis. As the infectious complications in these patients often are not life-threatening and neutropenia in children is usually self-limiting, therapy with antibiotics is sufficient in most patients. In more severe cases interventions with high doses of intravenous immunoglobulin or corticosteroid therapy are indicated.

Agranulocytosis↗

PubMedDate not supplied

[Immune granulocytopenia in children].

Immune neutropenia in infancy comprises alloimmune neutropenia and autoimmune neutropenia. Both diseases are caused by granulocyte-specific antibodies. Patients are newborns and children, often being younger than three years. Neutropenia usually leads to recurrent mild bacterial infections. Beside the blood count and the bone-marrow smear demonstration of granulocyte-specific antibodies is typical. As neutropenia is self-limiting, most patients require only conservative management. In more severe cases interventions with high-doses of intravenous immunoglobulin or corticoid-therapy are indicated.

Agranulocytosis↗