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J C Banzhaf

Publications and source records attributed to J C Banzhaf.

9 recordsLinked to original sources

Quality assurance in the blood bank. Studies of technologist performance.

A series of experiments was conducted to assess the performance of technologists in the blood bank, with particular reference to precision and accuracy in the technical and mechanical aspects of routine work, including preparation of erythrocyte suspensions, pipetting, interpretation of hemagglutination, detection of mixed-field agglutination, detection of weak alloantibodies, and antibody titration and scoring. The authors conclude that use of semiquantitative technics contributes significantly to imprecision in blood bank work. A plea is made for expediture of increased effort in quality assurance programs directed toward the technical staff.

Antibodies↗

Immunologic studies of blood samples transported by a pneumatic tube system.

The authors evaluated the suitability of using a pneumatic tube system for transport of patient blood samples to a hospital blood bank. Hemolysis and occasional reduction in serum antibody reactivity were found in some clotted blood samples after tube transport. Modifications would be necessary to make the system acceptable for routine blood bank use.

Blood Group Antigens↗

Ocurrence of anti-Wr a in blood donors and in selected patient groups, with a note on the incidence of the Wr a antigen.

The occurrence of the Wr a antigen on the red blood cells of 5,000 primarily Caucasian New York State blood donors was shown to be 1:1,000. Anti-Wr a was detected in sera from random blood donors with a frequency of 1:56 and with progressively increasing frequency in postpartum women, patients undergoing open-heart surgery, hemodialysis subjects, and random persons with other alloantibodies. Anti-Wr a was found in one fourth of sera from patients with positive direct antiglobulin tests, including 12 of 23 in whom the positive test was associated with alphamethyldopa treatment.

Adult↗

Quantitative studies of specific and nonspecific second antibody uptake by sensitized erythrocytes.

Experiments were conducted using the technique of quantitative hemagglutination with an electronic particle counter to study the specific and nonspecific uptake of blood group antibodies by previously sensitized erythrocytes. Specific attachment of a second antibody was impaired by prior RBC sensitization. It was found possible to quantitate simultaneously the amount of both specific and nonspecific second antibody uptake.

ABO Blood-Group System↗

A comparison of six procedures for compatibility testing.

Six procedures for compatibility testing were compared, using red blood cells sensitized in normal saline, albumin or low-ionic strength saline (LISS) combined with either broad spectrum or anti-IgG antiglobulin testing (AGT). Despite shortened incubation times, tests employing LISS were as sensitive as those with albumin and broad spectrum AGT and more sensitive than the other four combinations. No false positive results were found. LISS technique with broad spectrum AGT appears to be suitable and superior for routine antibody detection and compatibility tests.

Blood Grouping and Crossmatching↗