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Biomedical subjects

J C Baumgartner

Publications and source records attributed to J C Baumgartner.

At least 55 records · Page 3Linked to original sources
PubMed1991

Reactivity of IgG from explant cultures of periapical lesions with implicated microorganisms.

The presence of IgG in periapical inflammatory lesions suggests that immune responses participate in the disease process. The purpose of this investigation was to study the reactivity of IgG from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. Ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. A dot-enzyme-linked immunosorbent assay (dot-ELISA) was used to demonstrate the presence of IgG in the supernatant fluids of the explant cultures reactive with a panel of microorganisms associated with infections of endodontic origin. The percentages of reactivity by dot-ELISA follow: Bacteroides intermedius (84%), B. buccae (12%), Porphyromonas (Bacteroides) gingivalis (50%), P. endodontalis (58%), P. asaccharolyticus (17%), Peptostreptococcus micros (44%), P. anaerobius (26%), Eubacterium alactolyticum (34%), Fusobacterium nucleatum (14%), and Actinomyces israelii (6%). At least one of the three species of B. intermedius, P. gingivalis, or P. endodontalis tested gave a positive dot-ELISA with 89% of the supernatant fluids from explant cultures of periapical lesions. A lack of cross reactivity of IgG in supernatant fluids from explants of periapical lesions was demonstrated for the four strains of black-pigmented Bacteroides/Porphyromonas by dot-ELISA.

Actinomyces↗
PubMed1991

Bacteria in the apical 5 mm of infected root canals.

Ten freshly extracted teeth which had carious pulpal exposures and periapical lesions contiguous with the root apex were placed inside an anaerobic chamber and the apical 5 mm of the root canals cultured. In addition to anaerobic incubation, duplicate cultures were incubated aerobically. Fifty strains of bacteria from the 10 root canals were isolated and identified. The most prominent bacteria cultured from the 10 root canals were Actinomyces, Lactobacillus, black-pigmented Bacteroides, Peptostreptococcus, nonpigmented Bacteroides, Veillonella, Enterococcus faecalis, Fusobacterium nucleatum, and Streptococcus mutans. Of the 50 bacterial isolates, 34 (68%) were strict anaerobes. This study demonstrates the presence of predominantly anaerobic bacteria in the apical 5 mm of infected root canals in teeth with carious pulpal exposures and periapical lesions.

Bacteria, Anaerobic↗
PubMed1991

Biosynthesis of IgG in periapical lesion explant cultures.

The presence of immunoglobulins has been demonstrated in periapical inflammatory lesions associated with endodontic disease. The purpose of this study was to determine if IgG is synthesized in vitro in explant cultures of untreated periapical inflammatory lesions and to determine the level of IgG in isolated samples. Periapical lesions associated with infected root canals were removed from the roots and cultured in tissue culture medium containing tritiated amino acids. Supernatant fluids from the explant tissue cultures were passed through staphylococcal protein A affinity columns to isolate IgG. When the staphylococcal protein A eluents (24-h samples) from six periapical lesions were used in double diffusion in agarose assays, the presence of IgG was demonstrated in all the samples. Radial immunodiffusion assays to quantitate the IgG in staphylococcal protein A eluents showed that the levels of IgG detected in each successive daily supernatant fluid always decreased or else fell below the lower limits of detection. The in vitro biosynthesis of IgG in explant cultures of periapical lesions was demonstrated by the incorporation of tritiated amino acids into isolated IgG.

Analysis of Variance↗
PubMed1991

Microbiologic and pathologic aspects of endodontics.

Oral microorganisms, either directly or indirectly, produce the majority of cases of pulpal and periapical disease. Because microorganisms play such an important role in the pathogenesis of pulpal and periapical disease, a fundamental understanding of the disease process is important. Culture studies have identified infections of endodontic origin as being polymicrobial and predominantly anaerobic. Although several species of bacteria have been associated with infections of endodontic origin, there is no absolute correlation with signs and symptoms. The use of immunologic studies in addition to traditional microbiologic studies provides a better understanding of the mechanisms involved in the pathogenesis of pulpal and periapical disease.

Animals↗
PubMed1989

The use of bone imaging to detect a periapical lesion of endodontic origin.

This article reports the detection of a lesion of dental origin in the mandible of a 41-yr-old male patient during a routine bone scan used as a follow-up to cancer therapy. Unfortunately, more than 1 yr passed before the patient's signs and symptoms became severe enough for a definitive diagnosis to be made. A comprehensive dental examination is indicated for patients with positive bone scans in the area of the oral cavity. The use of bone imaging can be recommended to complement the dental examination of high risk patients with suspected metastasis or when all routine dental diagnostic measures are inconclusive.

Adult↗
PubMed1982

Dental treatment and management of a patient with a prosthetic heart valve.

The American Heart Association committee recognizes that their regimens do not cover all situations. Each patient should be evaluated individually. The necessity for parenteral therapy or oral erythromycin to continue for 48 hours after all dental procedures should be reevaluated on a case-by-case basis. On the other hand, if the dental manipulation involves an infected site, perhaps antibiotic therapy should be continued until signs of the inflammation subside. Dentists and physicians must use their clinical judgement in prescribing antibiotics. However, as Kaye proposed, any deviation in prescribing antibiotics should be in the direction of higher doses or more effective antibiotics. In addition, practitioners must provide adequate patient education and follow-up. Unfortunately, a negligent dentist, a physician giving poor or inadequate advice, or a patient not following instructions may turn a simple dental procedure into a life-threatening situation and potential tragedy.

Anti-Bacterial Agents↗