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J C Calamera

Publications and source records attributed to J C Calamera.

At least 37 records · Page 2Linked to original sources
PubMedDate not supplied

Spermatozoa motility and fructolysis--comparative study of samples obtained by masturbation and with plastic collectors.

A comparative study was made of spermatozoa motility and fructolysis with semen normal donors incubated in glass flasks and plastic collectors at room temperature (22 degrees to 25 degrees C). With the results obtained we came to the conclusion that poliethylene plastic collectors of low density, polimero 0.921, thickeness 20 to 30 mu, do not affect motility of fructose consumption of semen from normal donors. This material showed similar conditions to those in glass flasks.

Ascorbic Acid↗
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Adenosinetriphosphate (ATP) in human spermatozoa. II. Concentrations in fertile men.

The Bucher's method was applied to establish normal values of ATP in the spermatozoa of fertile men. It was shown that the normal value is 6.5 +/- 0.34 S.E. microgram per 10(8) spermatozoa. Pools of spermatozoa showed that incubation in seminal plasma at room temperature produced a progressive loss of ATP in the gametes.

Adenosine Triphosphate↗
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Effects of methyl xanthines upon the spermatozoa adenosinetriphosphate (ATP) concentration in normal semen.

The aim of the present study was to ascertain the effect of methyl xanthines upon the spermatozoa ATP concentration in normal semen in vitro. 26 normal semen were studied. The specimens were diluted after liquefaction with equal volume of Lopata's buffer or Lopata's buffer plus 1 or 6 mM either of caffeine or pentoxifylline. The samples of semen were incubated at room temperature during 90, 180 and 240 minutes before motility and ATP determination by the firefly luciferin-luciferase method. Significant variation was observed in sperm motility nevertheless variations in the ATP concentration was not induced by any of the methyl xanthines we used.

Adenosine Triphosphate↗
PubMedDate not supplied

Glycerylphosphorylcholine in selected forms of male infertility.

GPC was studied in the seminal plasma of 35 normozoospermic men, 34 cases of azoospermia due to bilateral deferent obstruction, 34 cases of azoospermia due to bilateral ejaculatory duct obstruction, 10 vasectomized patients, 6 vasectomized patients after vasovasostomy and 118 cases of spermatogenetic arrest without obstruction of the seminal ducts. Values of GPC in azoospermia due to deferent or ejaculatory ducts obstruction and to vasectomy are significantly lower than in normozoospermic subjects (p less than 0.001). Levels of GPC increased (p less than 0.05) following vasovasostomy. Although GPC values in secretory azoospermia were higher than those in cases of duct obstruction, they were still lower than in normozoospermic (p less than 0.001). The most likely source of GPC is the epididymis. These results support the assumption that GPC originates mainly in the epididymis. The absence of germinal cells in the epididymis could explain the decreased levels of GPC in azoospermia due to arrest of spermatogenesis.

Citrates↗
PubMedDate not supplied

Adenosine 5'-triphosphate (ATP) activity in normal and pathological human semen. Relationship with round cells of the ejaculate.

The aim of the present paper is deleted to determine ATP activity in the human seminal cells by bioluminescence and to investigate the relationship between these values and the number of round cells either peroxidase positive or peroxidase negative. We studied 244 untreated men, divided into six groups. Our mean value of ATP activity in normal semen was 20.02 +/- 0.65 n moles per 10(8) spermatozoa. In asthenozoospermic or oligoasthenozoospermic patients with less than 1,000,000 round cells per ml, the concentration of ATP was significantly lower than normal (P less than 0.001) or (P less than 0.05). Semen with normal characteristics or samples from asthenozoospermic or oligoasthenozoospermic patients but with more than 1,000,000 round cells, per ml and with predominance of peroxidase negative cells, had an ATP concentration higher than normal subjects (P less than 0.001). We believe that the knowledge of the quantity and quality of round cells of the ejaculate is important to interpret the seminal values of ATP.

Adenosine Triphosphate↗
PubMedDate not supplied

Adenosine 5'-triphosphate (ATP) concentration and acrosin activity in human spermatozoa. Their relation with the sperm penetration assay (SPA).

Fertility of men depends on the quality of semen. The aim of the present paper is to determine both the acrosin activity by radioimmunoassay and ATP concentration by bioluminescence in human spermatozoa, and evaluate these results in those samples with normal or low sperm penetration according to SPA test. Ejaculates obtained from 42 untreated men, were studied one hour after the obtention. These materials were divided into two groups:20 human semen samples with "in vitro" potentiality to penetrate zone-free hamster ova, between 15% to 98% and 22 human semen with SPA test between 0% to 14%. When we compare the group with normal penetration response vs that group with low or absent penetration ones, a significant decrease of ATP and acrosin concentrations was observed (P less than 0.001). Nevertheless no significant difference was observed in relation with percentage of motility, volume (ml), sperm concentration (10(6)/ml), percent of quick progressive spermatozoa and number of gametes capable of migrating into the medium layer (10(6)), between the group with low or absent penetration test against that one with normal zona-free hamster egg test.

Acrosin↗
PubMedDate not supplied

Adenosine 5 triphosphate (ATP) content and acrosin activity in polyzoospermic subjects.

The aim of this paper was to measure the concentration of spermatic ATP and acrosin in a group of patients with polyzoospermia, and to compare these values with those found in a group of normozoospermic men. The general characteristics of the semen, the ATP and the acrosin, were measured in a group of 46 men with polyzoospermia, who consulted in our clinic for sterility. The results were compared with those of 50 normozoospermic men in the control group. Statistically, no significant difference was found between the volume, motility and morphology of the samples in both groups; however, the concentration of spermatic ATP and acrosin decreased significantly in a group of polyzoospermic patients. This could be due to a functional defect of the spermatozoa in this group of patients.

Acrosin↗
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Comparison between swim-up and glass bead column techniques for the separation of human spermatozoa.

The glass bead column filtration method was compared with the swim-up technique regarding spermatozoa recovery in normal and pathological human semen. The general characteristics of the semen and the spermatic ATP concentration were measured in 67 patients. Samples processed with glass bead columns provided a good amount of motile forms which however, showed less velocity and linearity than in the case of using the swim-up technique. Additionally, the ATP determination in spermatozoa was included.

Cell Separation↗
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Development of an objective and manual technique to study the human sperm morphology.

The aim of the present paper is to present results of sperm morphology using an objective and manual technique by video image. Experiment 1:252 spermatozoa heads were measured in a microscope and in a monitor by each of three independent observers. The results allowed the calibration of an acetate overlay according to the WHO guideline and following the strict criteria. Experiment 2: 10 morphology slides from normal and abnormal patients were studied. These slides were evaluated by three independent observers, each counting at least 200 cells using the calibrate acetate overlay. In the first experiment the calculation of the regression out-put was: constant: 0.24, standard error of Yc: 0.04, R squared: 0.96, X coefficient: 0.36, and standard error of the coefficient: 0.03. In the second experiment, it can be seen that the differences among the operators are not statistically significant and therefore the experiment is independent from the operator. In conclusion, the methodology developed in this paper for the evaluation of morphology would be a good tool for the evaluation of human sperm morphology.

Cytological Techniques↗
PubMedDate not supplied

Development of an objective, semiautomated method for assessment of sperm motility.

Study of sperm motility is associated to the development of precise and economical systems of evaluation. The purpose of this work was to develop an Objective Semi-Automated Method (MOSA) to evaluate the sperm motility. Human semen samples were registered by video-microscopy. The same videofilms were analysed with the MOSA, the subjective method and the automated CellSoft method. The percentages of motile and immotile sperms were equivalent with the three methods. The percentages of rapidly and slowly motile sperms were similar both with the MOSA and the subjective method. The curvilinear and linear velocities as well as the linearity values obtained with the MOSA were different to those obtained with the CellSoft, although such differences would be biologically acceptable. MOSA is an inexpensive, objective and precise method that does not require trained technicians and allows evaluation of several parameters of sperm motility.

Equipment Design↗
PubMedDate not supplied

Effect of lipid peroxidation upon human spermatic adenosinetriphosphate (ATP). Relationship with motility, velocity and linearity of the spermatozoa.

The aim of the present paper was to study the effect of forced lipid peroxidation upon human spermatozoa and its relation with motility, velocity, linearity and ATP content. Forty-three pools of semen samples were studied one hour after liquefaction. After washing, normal and pathological spermatozoa presented no variations in motility, velocity or linearity with respect to basal values. Forced peroxidation produced a significant decrease of motility, velocity and linearity (p less than 0.02) with respect to control. Spermatic ATP was not significantly different in control vs forced peroxidation groups in normozoospermic samples. In contrast, a significant decrease of ATP between control and peroxidation groups was observed in asthenozoospermia at 120 minutes of incubation (p less than 0.02). In the asthenozoospermic group, a significant LPO-p increase was observed in comparison with the normal group.

Adenosine Triphosphate↗
PubMedDate not supplied

Computer assisted measurement in normal and pathological human semen, fresh and post swim-up technique.

A total of 429 semen samples were studied. Two hundred and twenty-nine samples with normal characteristics were processed following a basic procedure; the remaining 200 were normal and pathological samples that were analyzed pre and post swim-up. Semen specimens were allowed to liquefy for 30 minutes and sperm count, motility, velocity and linearity were determined using the Cellsoft Automatic Semen Analyzer. In normal patients, a significant increase of motility, velocity and linearity (p less than 0.001) post swim-up, was observed. Sperm recovery in this group was 13.9 +/- 1% of the whole motile sperm population. In the polyzoospermic group, recovery of motile spermatozoa post swim-up was significantly decreased as compared with the normal group (p less than 0.001). In the asthenozoospermic group (plus either hypospermia or hyperspermia, or with more than 30% of motile spermatozoa or less than 30% of motility), no variations of velocity or linearity as compared to the normal group were observed. In all the pathological groups studied, a significant increase in velocity and linearity (p less than 0.001) post swim-up, was observed.

Computers↗