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Biomedical subjects

J C Czyba

Publications and source records attributed to J C Czyba.

At least 55 records · Page 3Linked to original sources
PubMed1980

[Energy metabolism of spermatozoa: present status of the subject].

The energetic metabolism of the human sperm is characterized by the predominant importance of glycolytic pathway, with the probable participation of the pentose shunt. The participation of the aerobic pathway is very small since the oxidative phosphorylation seems to be partially uncoupled at time of ejaculation. There is no correlation between the respiratory activity and the mobility of the sperm. The effects of several physicochemical conditions were studied, indicating that the spermatozoon is indeed very resistant to variations of its environment. Seminal plasma seems to be a rather unsuitable medium for investigations of the glycolytic pathway, with the probable participation of the pentose shunt.

Adenosine Triphosphate↗
PubMed1979

Effect of tenotomy on self-reinnervated and randomly reinnervated soleus muscle of rat.

The time course and degree of atrophic changes caused by tenotomy were compared in normal, self-reinnervated and randomly reinnervated soleus muscle 6 months after transsection and reunion of the nerve at different distances from the muscle. Comparison was made between the behaviour of Type I and Type II fibers, distinguished on the basis of histochemical myofibrillar ATPase and succinic dehydrogenase reactions. Cross-sectional areas of individual muscle fibers were measured using Quantimet 720 image analyser. Selective atrophy of Type I muscle fibers as determined by structural and histochemical changes was observed after tenotomy of normal, self-reinnervated and randomly reinnervated soleus muscles after transsection of the muscular branch of the tibial nerve, Type II muscle fibers in randomly reinnervated muscles were found to be relatively insensitive to tenotomy, as in normal muscle. In randomly reinnervated muscles after transsection and reunion of the sciatic nerve, tenotomy did not cause any visible structural and histochemical abnormalities although a decrease of muscle weight and cross-sectional surface area of fibers was noted. Since in these muscles Type II fibers increased to about 70% of the muscle fiber population, it is suggested that the increased percentage of Type II fibers seemed to prevent the atrophic changes in Type I fibers after tenotomy.

Animals↗
PubMed1979

Effects of ions and K+:Na+ ratio on motility and oxygen consumption of human spermatozoa.

Variations in the osmolarity of the medium altered motility, velocity, and oxygen consumption of human spermatozoa. Variations in the K+:Na+ ratio also altered their motility and velocity. These alterations, which reflect both the composition of the medium and individual characteristics of the donor, are discussed in relation to the diversity of milieu through which the sperm are transported.

Humans↗
PubMed1979

Enzyme comparative study of spermatozoa and seminal plasma in normal and subfertile man.

A semiquantitative colorimetric micromethod (APIZYM) was used to study the enzyme profiles of seminal plasma and of spermatozoa. Reactions with 65 different substrates are simultaneously tested in a single specimen. These substrates (principally naphtolic) allow the detection of hydrolytic enzymes (esterases, phosphatases, and peptidases) and of dehydrogenases potentially involved in sperm metabolism and in the process of fertilization. The usual sperm enzymes were regularly observed: C3-C4 esterases, amino acid arylamidases, acrosine, phosphatases, glutamyl transpeptidase, and various osidases. Among the dehydrogenases we observed a striking predominance of the enzymes of the hexose monophosphate shunt and of LDH. Seminal plasma has an enzyme pattern very similar to that of spermatozoa except for the absence of acrosine and of some dehydrogenases. This unexpected similarity is discussed. The gametes from subfertile donors do not at first sight differ in their enzyme pattern from those from fertile donors. Moreover, we found no marked differences between zymograms of seminal plasma from normal, subfertile, or even azoospermic patients. Deep freezing does not modify the hydrolytic enzymes of human sperm either quantitatively of qualitatively, but the dehydrogenases of the hexose monophosphate shunt are adversely affected (60% loss of activity for G 6 PDH, and 30% for 6 PGDH); LDH is not affected. The consequences on fertilizing capacity of frozen semen are discussed.

Humans↗
PubMed1979

Psychological reactions of couples to artificial insemination with donor sperm.

The case histories of 62 couples successfully treated by AID were established through nondirective interviews, and were surprisingly similar in all cases. Several distinct stages were regularly observed: the awareness of wanting a child while the woman cannot become pregnant; the investigation of the couple's sterility with as a result, the announcement that the husband is sterile; a period in which the couple adapt to this situation; the disappearance of the feeling of guilt both of the husband, wounded in his "virility," and of the wife, ashamed of her desire for a child; acceptance of the idea of AID; request for AID; conflict with the medical profession; the disturbing seances of AID, usually in the presence of the husband; uneasiness at the beginning of pregnancy; euphoric continuation of pregnancy; uncomplicated delivery of a child whose father is very involved in its upbringing, and then request for a second child by AID.

Adult↗
PubMed1978

DNA content of cells in human buccal smears. A preliminary study.

The DNA content of the cells from human buccal smears, stained by the Feulgen method, was estimated by absorbtion microdensitometry using a Quantimet 720 D image analyser. It was concluded from measurements that most of the cells in buccal smears contained 2C DNA; however, among the cells from male donors a substantial proportion of cells (about 17%) contained less than 2C DNA. This difference between population of male cells versus female cells was statistically significant. The mean value of DNA content was estimated to be 7.7% lower for male cells than for female cells. The conclusions are preliminary because relatively small samples of cells have been measured.

Cheek↗
PubMed1978

[Disturbances in ovulation following on artificial insemination with donors (A.I.D.) (author's transl)].

The authors report on the results of statistical analysis of temperature curves in 139 patients before artificial insemination from donors (A.I.D.) and in the cycles in which they were inseminated. It appears that frequently the practice of A.I.D. causes ovulation to come early or late. Late ovulation seems to be the most frequent cause of failures which are then considered to be due to "psychogenic sterility".

Female↗
PubMed1977

[Effect of exogenous ADP and ATP on the motility and respiratory activity of human spermatozoa].

The respiratory activity of human sperms suspended in a salved solution, is varying when ADP or ATP are added to the medium. Those results seem to indicate that the apparent lack of regulation of the energetic metabolism, which is observed in this cell, could be partially due to low endogenous concentrations of nucleotide-phosphates. The enrichment of the medium with ATP does not lead to any effect on sperm motility and this negative result is discussed.

Adenosine Diphosphate↗
PubMed1976

[Modifications of motility and respiratory activity of human spermatozoa following congelation in liquid nitrogen].

By using a polarographic technique it has been noticed that the respiratory activity of human sperms was increased after addition of cryoprotector medium to semen. But this activity was markedly reduced after freezing in liquid nitrogen. We suppose that freezing causes a damage of some metabolic structures of cells which affects more severely their respiratory activity than their motility.

Cell Survival↗