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J C Czyba

Publications and source records attributed to J C Czyba.

96 records · Page 6Linked to original sources
PubMedDate not supplied

Evaluation of sperm motility by optical diffractometry of spermatograms.

Evaluation of sperm motility based on analysis of spermatograms by optical diffractometry was performed. Spermatograms are defined as images of sperm tracks obtained on microphotographs by dark field illumination with long exposure time. Single track analysis proved that the details concerning single tracks are "seen" and recognized by the technique of optical diffractometry. Normal "linear", as well as abnormal "irregular", including "circular", tracks were numerically described. Multitrack analysis: The general pattern of all tracks contained in particular spermatograms derived from two groups of semen classified by hospital laboratory as "high" (control) and "low" ("mediocre") motility of spermatozoa, respectively, were analysed by optical diffractometry. The hospital laboratory was asked to provide for diffractometric analysis samples of the "mediocre" semens of quality similar enough to the "control" ones what concerns the percentage of motile spermatozoa (40 percent) and spermatozoan concentration. Diffraction patterns of 102 spermatograms of control sperm and of 103 spermatograms of sperm qualified as "mediocre" by subjective microscopic evaluation of sperm motility, were analysed and compared. Using discriminant analysis based on parameters describing radial distribution of light intensity in diffraction patterns of control and "mediocre" spermatograms, it was possible to classify correctly 71 cases as high motility sperms out of 102 spermatograms evaluated beforehand by hospital laboratory criteria as control ones. In the group of 103 spermatograms classified as "mediocre" sperms by hospital laboratory criteria 69 cases were correctly recognized as "mediocre" by diffractometric analysis. The overlapping of the analysed two groups of sperm by 30 percent might be explained by the fact that in the control spermatograms many trajectories are formed by inefficient, slow moving spermatozoa.(ABSTRACT TRUNCATED AT 250 WORDS)

Humans↗
PubMedDate not supplied

Kinetic activity of spermatozoa from fertile subjects and asthenozoospermic infertile patients.

The motility characteristics of spermatozoa from asthenozoospermic semen were investigated and compared to the same parameters in fertile semen. The motility characteristics assessed by the CellSoft semen analyser (CRYO Resources Ltd, NY) were the following: curvilinear velocity (VCL), straight line velocity (VSL), amplitude of lateral head displacement (ALH), linearity (%LIN), and beat cross frequency (BCF). Analysis of the data indicated a decreased kinetic activity in the spermatozoa from the asthenozoospermic group which is expressed as a highly significant decrease (P < 0.002) in the VCL and VSL compared to velocities from normospermic samples. Moreover, percentage linearity and ALH were also statistically lower (P < 0.05) in this group. However, no difference was evidenced for the BCF.

Humans↗
PubMedDate not supplied

[Seasonal variations of cellular composition of human semen (author's transl)].

Sperm samples taken fortnightly from 14 young europeans over a period of 8 months show evidence of a seasonal variation in the concentration of spermatozoa and in the frequency of abnormal sperm (teratospermy). Both factors are highest in winter and lowest in springtime. A study of 3,000 spermograms established during consultations for sterility over the last 2 years did not clearly reveal the same trends, however, a statistical analysis of the results leads to the conclusion that several hundred examinations per month would be needed to show a significant seasonal variation in such a heterogeneous sample.

Adolescent↗
PubMedDate not supplied

[Relationships between seminal enzymes and fertility (author's transl)].

Various enzyme activities can be detected in human seminal plasma; these are mainly hydrolases which traduct high proteolytic activities. Acid phosphatase constitutes the best prostatic marker, while alpha glucosidase characterizes well epididymal function. The enzymatic equipment of spermatozoa -excepted for acrosin- is very similar to that of seminal plasma, which suggests that most of sperm enzymes are incorporated from male genital secretions. Tight relationships exist between the enzymatic equipment of spermatozoa and their fertilizing capacity: motility is depending of enzymes contained in sperm mid piece and flagellum; acrosomal enzymes are necessary for penetration of oocytes. However we could only very rarely explain some case of male sterility by defect of semen enzymes.

Acid Phosphatase↗
PubMedDate not supplied

Applications of laser scanning confocal microscopy to the observation of human oocytes and embryos.

Laser scanning confocal microscopy (LSCM) is a powerful and useful tool in developmental biology. The diverse applications of LSCM in biomedical field has led to advances in the microscopes themselves and the synthesis of novel specific probes for the observation of biological structures and the hypothesis of physiological process. LSCM was used to visualize the cellular actin cortex together with the chromatin in human arrested preimplantation embryos and in unfertilized oocytes obtained by in vitro fertilization. In embryos, we observed a majority of multinucleated blastomeres and some with fragmented nuclei or anucleated. LSCM also showed nuclei displaying the chromatin condensation and fragmentation patterns suggesting the phenomenon of apoptosis. Moreover, in arrested fragmented embryos of poor morphology, unequal sized blastomeres often showed cellular fragments without nuclei, compatible with aspects of apoptotic bodies.

Actins↗