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J C Frot

Publications and source records attributed to J C Frot.

8 recordsLinked to original sources
PubMed1984

Human anti-cytomegalovirus (CMV) immunoglobulins secreted by EBV-transformed B-lymphocytes cell lines.

The in vitro production of human immunoglobulins against cytomegalovirus may have clinical potentials. The attempts to produce human monoclonal antibodies by somatic cell hybridization have been unsuccessful so far. Another approach is to establish B-lymphoblastoid cell lines secreting specific antibodies. Usually such cell lines have been initiated after enrichment of antibody producing B-cells before immortalization by Epstein-Barr virus. We investigated the possibility of establishing lines secreting antibodies neutralizing human cytomegalovirus infectivity by selecting leucocyte donors who have undergone clinical disease which resulted in natural enrichment of antibody producing cells. Two cell lines were established from a patient with a severe post-transfusional CMV syndrome and one cell line from a patient who has continuously shed CMV since renal transplantation 10 years ago. The characterization of the specific immunoglobulin production of these lines will be presented.

Antibodies, Viral↗
PubMed1984

[The concept of protein profile].

From their own experience of the simultaneous immunonephelometry of eight serum proteins, the authors propose a definition of protein profile from the point of view both of laboratory technique and interpretation. The assay should be performed quickly and the results expressed diagrammatically in normalised values, in such a way that the relative variation in protein levels can be easily visualised. A protein profile should thus comprise a minimum of proteins to be measured, chosen according to protein physiopathology and the type of abnormality under investigation. Interpretation is based on inter-protein correlations that may appear or disappear depending on the underlying physiopathology. This makes it possible to study inter-protein variation and thus avoid the probabilistic "interpretation" of single proteins taken in isolation. From this approach syndromes can be divided into two groups, elementary or complex. The authors provide examples of each, and propose an interpretation model based on this dichotomy.

Blood Protein Electrophoresis↗
PubMed1983

[Gammopathies with oligoclonal electrophoretic patterns. Incidence, immunochemical nature and association with neoplastic pathology].

Electrophoretic study of 680 cases of human sera containing monoclonal bands showed an oligoclonal pattern in 92 cases (13.5%). Immunoelectrophoretic analysis of 52 oligoclonal cases showed various patterns: polyclonal in 12 cases, monoclonal in 23 cases, monoclonal with associated Bence Jones proteinemia in four cases, and biclonal in only 13 cases. In cases lacking immunoelectrophoretic evidence for the oligoclonal nature of gammopathy, a restricted heterogeneity in molecular weight of subunits was evidenced in 12 out of 27 cases. Associated diseases were investigated comparatively in the monoclonal and the oligoclonal groups. A significant increase in cancer aetiology was found in the oligoclonal group (p less than 0.0005).

Electrophoresis↗
PubMed1983

[Decrease of the 3rd fraction of serum complement. Study in a hospital population of 13000 patients].

Patients with serum concentrations of C3 lower than 0.40 g/l among 13 000 patients in a general hospital seen over an 18 months period were studied. 95 cases were eligible for study. Diffuse and severe liver disease accounts for 50% of cases. Immunologic diseases represent little more than 10% of cases. The other common causes are severe infections and nutritional deficiencies. Besides the immunological diseases, low C3 serum concentration represents a poor prognosis factor since 55% of patients died during hospitalization.

Complement C3↗
PubMedDate not supplied

Prenatal diagnosis of cystic fibrosis. I. Prospective study of 51 pregnancies.

A prenatal diagnosis was performed in 51 pregnancies with a 1-in-4 risk of having a child with cystic fibrosis. The criteria for determining an affected fetus were based on the results of alkaline phosphatase (ALP) residual activity after inhibition by phenylalanine and by homoarginine, of total ALP activity, and of gamma-glutamyltranspeptidase (GGTP) activity in the amniotic fluid taken between 16 and 19 weeks of pregnancy. The chromosomal analysis of amniotic fluid cells showed trisomy 13 in one case which was excluded from the analysis of biochemical assays. The biochemical assays were in the normal ranges in the amniotic fluid of 35 pregnancies: 26 have reached term and a normal infant has been born, 9 are still in progress. A deficiency of the ALP phenylalanine-inhibitable form, depressed values of total ALP and GGTP were observed in the amniotic fluid of 15 pregnancies: one pregnancy went to term and the infant had CF, in 14 cases the pregnancy was terminated, and meconium ileus was observed in ten of these cases. It was observed that the changes towards abnormal values became more significant with advancing gestational age and that 18 weeks appeared to be the optimum time for diagnostic amniocentesis.

Abortion, Therapeutic↗