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J C Iezzoni

Publications and source records attributed to J C Iezzoni.

28 records · Page 2Linked to original sources
PubMed1996

Ovarian granulosa cell tumors with bizarre nuclei: an immunohistochemical analysis with fluorescence in situ hybridization documenting trisomy 12 in the bizarre component [corrected].

Granulosa cell tumors with bizarre nuclei (GCT-BN) are rare lesions with a prognosis apparently similar to that of conventional granulosa cell tumors (GCT-NOS). The immunohistochemical features of GCT-BN have not been described, and the exact nature of the bizarre nuclei (BN) is unclear. Thirteen GCT-BN were studied with antibodies to cytokeratin, vimentin, epithelial membrane antigen, muscle-specific actin, alpha smooth muscle actin, desmin, and S-100 protein. Six cases were also examined by fluorescence in situ hybridization for trisomy 12, a nonrandom chromosomal aberration found in a proportion of ovarian sex-cord stromal tumors. Histologically, 12 tumors (86%) contained BN areas interspersed with large areas of GCT-NOS. The remaining tumor contained only microscopic foci of GCT-NOS. Immunohistochemically, the tumors stained for vimentin (13 tumors), S-100 protein (11 tumors), muscle-specific actin (10 tumors), cytokeratin (eight tumors), alpha smooth muscle actin (eight tumors), and desmin (one tumor), but none stained for epithelial membrane antigen. Immunostaining results for the BN and GCT-NOS areas were concordant in eight (73%) of the 11 tumors in which both areas could be independently assessed. The remaining three tumors (27%) showed discordant results for only one of the eight markers used. In five patients, trisomy 12 was detected by fluorescence in situ hybridization in areas of BN but not in areas of GCT-NOS present in the same tumor. Trisomy 12 was also present in another BN tumor in which the foci of GCT-NOS were too small to be evaluated. We conclude that within GCT-BN, areas with BN are immunohistochemically similar to areas of GCT-NOS present in the same tumor. The finding of trisomy 12 in areas with BN but not GCT-NOS in the same tumor, however, suggests that cells with BN represent a genetically distinct clone of tumor cells arising within GCT-NOS.

Adolescent↗
PubMed1996

Thymic basaloid carcinoma: a case report and review of the literature.

We report a case of thymic basaloid carcinoma in a 65-yr-old man who presented with symptoms referable to the mass effect of the tumor. The tumor arose in association with a multilocular thymic cyst and microscopically was characterized by islands, nests, and cords of basaloid cells. The tumor cells demonstrated prominent palisading around most of the neoplastic islands and nests. The tumor was excised, and there was no evidence of recurrent or metastatic disease in the 6-mo follow-up period. Including the current case, seven cases of basaloid thymic carcinoma have been reported in the English literature, four of which (57%) were associated with a multiloculated thymic cyst. Follow-up was available on three patients. Although no patient has died of tumor, pulmonary metastases developed in two patients. Although basaloid thymic carcinoma is currently considered a tumor of low-grade histology, more cases need to be studied to determine the long-term prognosis of these tumors.

Aged↗
PubMed1995

Cyclin D1 (PRAD1, CCND1) and glutathione-S-transferase pi gene expression in head and neck squamous cell carcinoma.

Chromosome 11q13 amplification has been identified in a subset of head and neck squamous cell carcinomas (H&N SCCs). This region contains several putative oncogenes, including cyclin D1 (PRAD1, CCND1), which encodes for an important cell cycle regulatory protein, and the locus encoding for the drug-detoxifying enzyme glutathione-S-transferase-pi (GST-pi). To determine the relationship of cyclin D1 and GST-pi gene amplification to expression of the encoded proteins, the authors examined 64 H&N SCCs by both Southern blot hybridization and immunohistochemistry, using a recently described, affinity-purified, anticyclin D1 polyclonal antibody no. 19 as well as a polyclonal antibody against GST-pi. Anticyclin D1 antibody no. 19 labeled the tumor cell nuclei in 28 (44%) of the H&N SCCs, whereas cytoplasmic immunoreactivity for GST-pi was noted in 55 (86%) neoplasms. By Southern blot 24 tumors (37.5%) showed twofold to tenfold amplification of 11q13 loci; only two of these were coamplified for GST-pi. Immunopositivity with anticyclin D1 antibody no. 19 but not anti-GST-pi significantly correlated with 11q13 amplification (P < .0001). Of the 28 tumors positive with anticyclin D1 antibody no. 19, however, only 18 (64%) were amplified for 11q13, and six amplified tumors did not react with the no. 19 antibody. A strong trend was noted between anticyclin D1 antibody no. 19 reactivity and a hypopharyngeal primary site (P = .053), but no correlations were observed between immunoreactivity and cytological grade, architectural pattern, pathological stage, and disease-free or overall survival. The inconsistent association of cyclin D1 immunoreactivity with 11q13 amplification indicates that other mechanisms may exist for protein overexpression. Immunoreactivity for the GST-pi protein is prevalent in H&N SCC but is clearly unassociated with amplification. In this series, the presence or extent of cyclin D1 and GST-pi immunoreactivity was of no proven prognostic benefit in H&N SCC.

Adult↗
PubMed1995

The role of Epstein-Barr virus in lymphoepithelioma-like carcinomas.

Epstein-Barr virus (EBV) has been implicated in the pathogenesis of a variety of lymphoproliferative disorders and several epithelial neoplasms, including undifferentiated nasopharyngeal carcinoma (UNPC; lymphoepithelioma). Lymphoepithelioma-like carcinomas (LEC) are tumors with morphologic features identical to UNPC that occur outside the nasopharynx. To determine whether EBV is associated with LEC, the authors conducted a comprehensive literature review of all pathologically documented LEC reported to date in the English literature. In summary, EBV is associated consistently with LEC from only four anatomic sites: stomach, salivary gland, lung, and thymus. Racial and/or geographic factors influence the association of EBV with LEC in some of these organs. Specifically, the association of EBV with LEC of the salivary gland and lung is restricted to Asian patients, whereas the association of EBV with gastric and thymic LEC is independent of race. The presence or absence of EBV in LEC does not appear to be prognotically important in those cases studies to date.

Breast Neoplasms↗
PubMed1995

Aggressive angiomyxoma in males. A report of four cases.

Aggressive angiomyxoma (AAM) is a rare, locally infiltrative but nonmetastasizing tumor of the pelvic and perineal soft tissues that occurs almost exclusively in adult females. The authors describe four cases of AAM in adult males that arose in the scrotum. There was some histologic variation among the tumors. One case was focally hypercellular around the blood vessels, two were more densely cellular throughout, and one had cystic degeneration. Three of the tumors were widely infiltrative, and one of the four tumors recurred locally. The clinicopathologic features of these cases are similar to those of AAM occurring in females. In either sex, AAM should be distinguished from benign myxoid tumors with a low risk of local recurrence and fully malignant myxoid tumors with distant metastatic potential.

Adolescent↗
PubMed1995

The biopsy. The pathologist's point of view.

The science of medicine is constantly evolving. Surgical pathology must respond to these changes. For example, incisional biopsies once taken during an open surgical procedure are now being replaced by FNA biopsies performed on the outpatient. Pathologists are being asked to diagnose lesions on smaller pieces of tissue and fewer cells. Advances in molecular biology are allowing diseases to be examined at the molecular level. The pathologist is now asked to integrate this molecular data into his or her diagnostic process (e.g., lymphomas). The impact on the pathologist of the increased complexity imposed on examination of tissues cannot be overstated. Despite these difficulties, diagnosis always comes down to the integration of the morphologic features with the clinical findings. As such, the diagnosis will always depend on close teamwork between the surgeon and the pathologist.

Biopsy↗
PubMed1993

Rapid colorimetric detection of epidermal growth factor receptor mRNA by in situ hybridization.

We describe a rapid, formamide-free, random oligomer-enhanced in situ hybridization method in formalin-fixed, paraffin-embedded tissue sections using a biotinylated oligonucleotide probe for colorimetric detection of the mRNA transcript of the epidermal growth factor receptor (EGFR) gene, a putative protooncogene. Transitional cell carcinomas (TCC) of the urinary bladder and oral squamous cell carcinomas (SCC) were examined. A431, a human squamous carcinoma cell line that overexpresses EGFR mRNA, and mature skeletal muscle, known to express EGFR, served as control tissues. A biotinylated poly-T oligonucleotide probe was used to evaluate the preservation of mRNA in the formalin-fixed, paraffin-embedded tissues. The EGFR mRNA and poly-T oligonucleotide probes were constructed with a target-specific, 5' region and a 3' non-hybridizing, biotinylated tail. Random sequence oligomers nine bases long added to the probe cocktail eliminated the need for formamide and sheared carrier DNA in the method. The assay produced strong, specific staining for EGFR mRNA in all cases of TCC and SCC, and in the A431 tumors, but not in skeletal muscle. The assay was completed in approximately 90 minutes. This method may have widespread utility for rapid and specific detection of other mRNA sequences.

Base Sequence↗
PubMed1993

Sarcomatoid carcinomas (carcinosarcomas) of the gastrointestinal tract: a review.

This review discusses sarcomatoid carcinomas of the gastrointestinal tract, rare malignant neoplasms that demonstrate both epithelial and mesenchymal differentiation. The clinical, histological, immunohistochemical, and ultrastructural features are described. Practical problems in the differential diagnosis of these tumors are also discussed.

Adult↗