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J C Lissitzky

Publications and source records attributed to J C Lissitzky.

60 records · Page 4Linked to original sources
PubMed1978

[Transsphenoidal surgery in hypersecreting pituitary tumors: endocrine follow-up (author's transl)].

Among 18 patients suffering prolactin adenoma and 16 acromegalic patients, plasma levels of PRL and HGH respectively returned to normal vlues (PRL = 9 +/- 2 ng/ml HGH = 4.2 +/- 0.8 ng/ml) immediately after selective removal of the pituitary adenoma by transsphenoïdal route. In all cases tumoral symptoms were reduced. Menses were recovered 36 +/- 5 days after surgery in all patients with prolactin adenoma. Pregnancy succeeded in nine of these women. Per-operative studies of HGH and PRL plasma variations proved to be of good prognostic value. The evaluation of the pituitary functions was performed before and after surgery. In no case pituitary deficiency occured after treatment. Furthermore the regulation of somatotropic and prolactin functions have been documented by the use of different provocative tests before and after treatment. They allow discussion of the pathogenecity of these hypersecreting tumors.

Adenoma↗
PubMedDate not supplied

Laminin-mediated adhesion in metastatic rat rhabdomyosarcoma cell lines involves prominent interactions with the laminin E8 fragment.

In vitro attachment assays were carried out to assess adhesion between two basement membrane proteins, type IV collagen and laminin, and rat rhabdomyosarcoma (RMS) cell lines with different metastatic potentials. Whereas cells did not adhere to type IV collagen, adhesion to laminin appeared to be very sensitive as maximal adhesion was achieved in dose-response assays with only nanograms of laminin. Adhesion was mediated by interactions between coated laminin and cell surface components, probably receptors, but not endogenous laminin. Laminin-mediated adhesion of RMS cell lines was compared with that of the MCF-7 (human mammary carcinoma) and the L6 (rat myoblast) cell lines. In dose-response assays, RMS cell lines required 10 times less laminin to reach half-maximal attachment rates than MCF-7 and L6 cell lines. Two laminin fragments, P1 and E8, which are structurally and immunologically distinct as shown by alpha-helix content, SDS-PAGE and monoclonal antibody mapping, supported adhesion by RMS cells and L6 myoblasts, but MCF-7 adhered only to P1. This fragment was 10 times less active than laminin in RMS cell lines. Attachment in dose-response assays and adhesion inhibition studies by antibodies revealed that E8 accounted for the activity of laminin in RMS cell adhesion. Adhesion in the RMS cell lines was dominated by interaction with E8 regardless of metastatic potential.

Animals↗
PubMedDate not supplied

Modulable Southern and Northern blot hybridization apparatus for routine screening of gene amplification and expression.

An apparatus for Northern and Southern blot hybridization is described. It allows from one to twenty-four blots to be processed at the same time, with different probes. All the pre-, post- and hybridization steps are performed without handling the filters and the experimenter is totally protected from beta radiations. The development of such modulable materials has become necessary since Southern and Northern techniques are becoming routine assays in hospitals, particularly in the field of oncology, in prognosis and for hereditary diseases, as an antenatal diagnosis procedure.

Blotting, Northern↗
PubMedDate not supplied

Regulation of opioid gene expression.

Opioid peptides are synthesized in the form of large precursors, which contain the information for more than one biologically active peptide. Using recombinant DNA technology, three opioid precursors have been sequenced: pro-opiomelanocortin (POMC), proenkephalin and prodynorphin. Analysis of the structures of these three precursors and their corresponding genes show striking similarities suggesting a common evolutionary mechanism. Regulation of POMC gene expression has been analyzed in different rat tissues. Detection of POMC mRNA in brain tissues supports the hypothesis that ACTH and endorphin peptides are synthesized in these tissues. Quantitation of POMC mRNA levels in pituitaries of rats subjected to adrenalectomy and glucocorticoid treatment shows that the feedback effect of the glucocorticoids occurs at the level of the rate of transcription of POMC mRNA.

Adrenalectomy↗