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J C Petithory

Publications and source records attributed to J C Petithory.

45 records · Page 3Linked to original sources
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[A challenged Nobel Prize: Johannes Fibiger, 1926].

Johannes Fibiger born in Denmark in 1867 died in 1928 from a cancer of the colon. First interested in bacteriology he became later (1900) professor of pathological anatomy. His chief work on the alleged cancerigenous role of a nematode Gonglyonema neoplasticum in some species of rats allowed him to receive the Nobel Prize in medicine in 1926. The difficulties met later (species of Gongylonema spp. from rats, dietary, lack of vitamin A) and even the impossibility to reproduce his results have brought a contestation of his work. However the cancerigenous action of some parasitic heminths such as Schistosoma is now recognized.

Denmark↗
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[Preparation of a standardized antigenic extract from several strains of African trypansomes].

The preparation of a soluble antigen from african trypanosomes is described below. It is important to observe the following steps: --minimal dose of the phytohemagglutinin must be placed in rapid contact with the trypanosomes; --the standard speed of the different centrifugations must be strictly observed; --a thorough washing of the trypanosomes must be carried out to eliminate the hetero-antigens, but caution should be excercised, as over washing is harmful to the Trypanosomes.

Animals↗
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[Immunologic diagnosis of ocular larva migrans syndrome].

To elicit the differential diagnosis between retinoblastoma and ocular larva migrans, we have realized an immunological diagnosis detecting antibodies in the endoocular fluids, using gel precipitations methods (electrosyneresis, Ouchterlony, immunoelectrophoresis) with the antigens Excretions-Secretions of T. canis and T. cati larvae. We have got 8 positive results out of eight cases of ocular larva migrans and no false reaction out of 11 control sera. Theses methods appear to be better than E.L.I.S.A. because it is difficult to define the threshold value for this technic.

Adult↗
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[Comparative value of immunofluorescence and immunoenzymology on figured antigen in the immunological diagnosis of African trypanosomiasis].

Indirect immunoperoxydase method is compared with indirect immunofluorescence using immunized animal and human sera, and negative control sera. The obtained results show a good statisticly established concord and allow to propose the indirect immunoperoxydase method being used in the biological diagnostic of african Trypanosomiasis because its easy carrying out and its examin with an ordinary microscope.

Antigens↗
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[Comparative study of immunoenzyme technics, ELISA and on formed antigens, in the immunologic diagnosis of African trypanosomiasis].

The enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of human african trypanosomiasis is described, comparatively with the immunoperoxidase technique using blood streamforms of Trypanosoma brucei as antigen. Practical aspects for measuring the amount of antibody in serum samples from infected rabbits and from patients with african sleeping sickness are discussed. ELISA does offer important advantages over the other method used namely: -- quantitative estimates are possible, -- it bends itself readily to large scale series, owing the fact that it can be fully automatic. These qualities will be turned to full account when the different classes of antibodies present in trypanosomiasis suffers can be examined by means of specific antigenic fractions.

Adolescent↗
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[Sero-immunologic diagnosis of human African trypanosomiasis: cross reactions of various parasitoses and hyperglobulinemias].

The immunological test: latex agglutination, counter immuno-electrophoresis, ELISA, and immunoperoxydase test, have been applied on the serodiagnosis of african sleeping sickness, and have been compared with each other, with special reference to the cross-reactions in parasitic diseases and hyperglobulinemia. In addition to malaria and leishmaniasis well known interferences, this study gives clear indication of the importance of false positive reactions in active toxoplasma infection. Any how, the serodiagnostic can be worked out from the analysis of responses to various specific antigens, which are always more positive with the homologous antigen.

Animals↗
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[Immunoglobulins in bilharziasis].

The increase of the level of IgE allows to suspect Schistosomiasis with a frequency of 68%. IgG level is increased in all types of Schistosomiasis (Schistosoma mansoni, Schistosoma haematobium and Schistosoma intercalatum). IgM level is increased in hepatosplenic disease in relation with Schistosoma mansoni. There is no significant variation of IgA level.

Hepatomegaly↗
PubMedDate not supplied

[Electrosyneresis in the diagnosis of African trypanosomiasis].

Comparative studies were carried out on serums from rabbits inoculated with several Trypanosoma strains and 50 serums from sleeping sickness cases. The techniques used were antigen-antibody reaction in gel according to Ouchterlony, and electrosynerese for which directions are outlined. Electrosynerese has been shown to be a much faster and more sensitive method of detecting precipitating antibodies in trypanosomiasis.

Animals↗