Biomedical subjects

J C Wataha

Publications and source records attributed to J C Wataha.

78 records · Page 5Linked to original sources

PubMed1991

The in vitro effects of metal cations on eukaryotic cell metabolism.

The in vitro cytotoxicity of nine metal cations common in dental casting alloys was evaluated using Balb/c 3T3 fibroblasts and four toxicity parameters: total protein production, 3H-leucine incorporation, 3H-thymidine incorporation, and MTT-formazan production. Concentrations causing 50% toxicity compared to controls (TC50's) and reversibility of these effects were determined. The range of potency of the metal cations was 2-3 orders of magnitude, with Cd2+ showing the greatest potency and In3+ showing the least. Potency did not correlate with atomic weight for these metals. For each metal cation, the TC50's of the various toxicity parameters were similar in most cases. However, several cations (Cu2+, Ga3+) showed greater potency with 3H-thymidine incorporation. Reversibility of the toxic effects was observed for all cations; the effects generally became irreversible at concentrations in the range of the TC50 value for each cation. Several stimulatory effects were seen. Small but statistically significant stimulations were observed after 24 h of metal exposure for Ag1+, Au4+, Cu2+, Ga3+, and Ni2+. Residual stimulations 24 h after removal of the metal cations were observed for Au4+, Cd2+, Ni2+, and Zn2+. Stimulations always occurred at concentrations below the TC50 concentrations. This study should be useful in evaluating the potential cytotoxic effects of metal cations released from dental alloys.

3T3 Cells↗

PubMed1991

The release of elements of dental casting alloys into cell-culture medium.

Ten dental casting alloys were tested for alloy-element release into cell-culture medium, and this release was related to alloy composition, alloy microstructure, and alloy cytotoxicity (previously determined). Cell-culture medium was analyzed for alloy elements by flame atomic absorption. Concentrations of elements in the medium were normalized by dividing them by their atomic abundance in the alloy, giving element medium-alloy ratios (EMA ratios). Results showed that Au, In, and Pd generally did not dissolve into the medium, but that Ag, Cd, Cu, Ga, Ni, and Zn frequently dissolved. Comparison of EMA ratios for Ag, Cu, and Zn showed that each element retained a behavioral identity in diverse metallurgical environments, but that these environments influenced the release behavior to some degree. Some EMA ratios in multiphase alloys were greater than those in solid solutions, and EMA ratios showed great diversity within all the alloys. Nominal composition seemed to be of little value in the prediction of metal release unless the composition supported multiple-phase formation. In addition, release of alloy elements did not, in itself, completely predict alloy cytotoxicity measured previously. However, cytotoxicity was associated with metal release in each case. The commercial alloys used in this study exhibited more complex and less predictable release behavior than did the simpler ternary alloy systems used by previous investigators. It is believed that the use of commercial preparations is necessary for their in vivo behavior to be modeled.

Copper↗

PubMed1991

Cytotoxic effects of resin components on cultured mammalian fibroblasts.

The objectives of this study were to determine the cytotoxic concentrations of 11 components of resin composites on monolayers of cultured Balb/c 3T3 fibroblasts, to study the inhibitory effects of these components on DNA synthesis, total protein content, and protein synthesis, and to determine whether effects were reversible when the components were withdrawn from the medium. These data were reported as concentrations which inhibited 10% (ID10) and 50% (ID50) of a particular metabolic process as well as the range of concentrations over which cell metabolism was irreversibly inhibited. For any individual component, the ID50 values for all three metabolic parameters were of the same magnitude. The same was true for the ranges of irreversibility. Ethoxylated Bis-phenol A dimethacrylate (E-BPA) was the most toxic molecule of the group (ID50 being between 1 and 10 mumol/L). The ID50 concentrations for three of the components, including Bis-GMA, UDMA, TEGDMA, and Bis-phenol A, ranged between 10 and 100 mumol/L, while the ID50 values of three components (N,N dihydroxyethyl-p-toluidine, camphoroquinone, and N,N dimethylaminoethyl methacrylate) were above 100 mumol/L. The concentrations to which the cells and tissues are exposed in vivo are not known. This study should help to identify the concentrations of organic composite components which pose clinical cytotoxic hazards.

Animals↗

PubMedDate not supplied

Element release and cytotoxicity of Pd-Cu binary alloys.

The release of elements from a series of palladium-copper alloys into cell-culture medium was measured using atomic absorption spectroscopy. The effect of the alloys on the succinyl dehydrogenase activity of Balb/c 3T3 fibroblasts was also measured. Palladium inhibited the release of Cu in alloys up to 70 atomic weight percent Cu. Below 70 at. % Cu, Cu release was < 0.8 micrograms/cm2 at 72 hours. Palladium release was lower from pure Pd (0.08 micrograms/cm2) at 72 hours than from Pd-Cu alloys containing > 70 at. % Cu, where it increased to 1.2 micrograms/cm2. The cytotoxicity of the alloys correlated closely with the release of Cu, becoming markedly cytotoxic above 70 at. % Cu, when Cu release was greater than 2.2 micrograms/cm2.

Animals↗

PubMedDate not supplied

Long-term cytotoxicity of dental casting alloys.

PURPOSE: Current in vitro cytotoxicity tests for dental casting alloys may be inadequate because they use a short-term test (generally < 168 h) for a material that is present in the mouth for years. A modified in vitro test was used to test the cytotoxicity of 8 dental casting alloys and 3 pure metals. This modified test attempted to simulate the long-term effects of dental casting alloys in the mouth. MATERIALS AND METHODS: Alloys were tested in cell culture either immediately after polishing (initial) or after 10 months of conditioning in a biologic medium containing serum proteins. The cytotoxicities of the 2 conditions were then compared. Alloys were repolished to assess the reversibility of the 10-month conditioning. RESULTS: Alloys with little initial cytotoxicity showed no 10-month cytotoxicity. Alloys with significant initial toxicity showed significantly less toxicity at 10 months. No alloy increased in toxicity after conditioning. Repolishing multiple times did not necessarily reestablish the initial cytotoxicity response. CONCLUSION: The results indicate that short-term cytotoxicity tests may not accurately measure the long-term cytotoxicity of these types of alloys.

3T3 Cells↗

PubMedDate not supplied

Correlation between cytotoxicity and the elements released by dental casting alloys.

Elements that caused cytotoxicity of high-noble, noble, and silver-based dental casting alloys were identified by correlating the release of elements with the cytotoxicities of the alloys. Release of elements was determined by atomic absorption spectroscopy, and the cytotoxic effect was determined by cellular mitochondrial function. The high-noble alloy released low levels of elements that did not increase after 6 hours, whereas the other alloys released higher levels of elements that increased over time. The cytotoxic effects of the alloys were attributed primarily to the release of silver and copper. In some observations, interactions between silver and copper were suspected of having an effect on the cytotoxicity.

3T3 Cells↗