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J Córdoba

Publications and source records attributed to J Córdoba.

50 records · Page 3Linked to original sources
PubMed1997

Stenosis of a portacaval anastomosis affects circadian locomotor activity in the rat: a multivariable analysis.

The study of hepatic encephalopathy is limited by the lack of standardized experimental models to assess behavior. We have shown that rats continuously monitored while running on a wheel show abnormalities of the circadian rhythm of locomotor activity after portacaval anastomosis (PCA), such that entrainment of running activity to the light-dark cycle is severely impaired. To identify factors that affect postoperative circadian behavior, we have performed a multivariable analysis of 69 sham-operated controls and 107 rats after PCA. Our results indicate that shunt stenosis, as determined by the pressure gradient from the splenic pulp to the inferior vena cava, ameliorated the postoperative deterioration of the circadian rhythm. In addition, postoperative behavior was affected by preoperative performance, diet, and gender. Postoperative body weight gain, spleen weight, and liver atrophy did not impact this model. Because shunt stenosis is known to ameliorate hepatic encephalopathy in humans, our findings support the validity of this behavioral end point as a correlate of hepatic encephalopathy. Measurement of the pressure gradient across the anastomosis and achievement of sufficient preoperative entrainment appear critical for the standardization of the model.

Animals↗
PubMed1997

Treatment of chronic hepatitis C with interferon-alpha. Clinical histological and virological implications.

OBJECTIVE: to identify pretreatment predictive factors of long-term biochemical and virological response to interferon-alpha in chronic hepatitis C and to determine the effects of a second course of interferon-alpha in patients who responded but relapsed after interferon withdrawal. DESIGN: retrospective analysis. SETTING: outpatient liver clinic of a tertiary hospital in Spain. PATIENTS: 112 patients with chronic hepatitis C were treated with recombinant interferon-alpha (3 MU three times a week for 6 months). Twenty-four patients who responded but relapsed after interferon withdrawal were treated with a second course of interferon (3 MU three times a week for 12 months). RESULTS: seventy-two patients were non-responders (64%), 11 patients had a sustained response (10%) and 29 patients responded but relapsed after interferon withdrawal (26%). Five (25%) of the 24 patients who relapsed and were treated with a second course of interferon experienced a sustained response (mean follow-up: 10 months). By multivariate analysis, four pretreatment variables were found to be predictive of a complete response: age < 40 years (p = 0.0004), history of IVDA (p = 0.001), low serum levels (p = 0.013), and genotype 3 (p = 0.01). Two variables were found to be predictive of a sustained response: short duration of HCV infection (p = 0.09) and genotype 3 (p = 0.01). Sustained responders appeared to have lower HCV-RNA levels than those with complete response who relapsed and non-responders. HCV viremia levels were not associated with the severity of liver histology, duration of disease or the source of hepatitis. CONCLUSIONS: in the present study a low sustained response rate was observed using a standard interferon-alpha regimen (3 MU three times a week for 6 months). The sustained response rate increased slightly with a second course of interferon-alpha (3 MU three times a week for 12 months) in patients with a complete response who relapsed after interferon withdrawal. Sustained response is related to viral genotype and duration of HCV infection.

Adolescent↗
PubMed1995

Hepatitis C virus infection in renal transplant recipients: epidemiology, clinical impact, serological confirmation and viral replication.

The presence and significance of hepatitis C virus infection was evaluated in 241 renal transplant recipients from our hospital. Hepatitis C virus antibodies were tested by second-generation enzyme immunoassay, followed by second- and third-generation immunoblot assays (RIBA-2 and RIBA-3); hepatitis C virus RNA was measured by nested polymerase chain reaction. Hepatitis C virus antibodies, which were detected in 46.5% of patients, were mainly present before transplantation and independently associated with the total amount of transfused blood, time in hemodialysis and duration of posttransplant follow up. Liver dysfunction (alanine aminotransferase elevation) was observed in 50% of antibody-positive recipients, and 92.5% of patients with chronic liver disease without hepatitis B infection were infected with hepatitis C virus. Most antibody-positive patients (78.4%) tested positive by RIBA-2, but 21.6% were indeterminate; RIBA-3 was positive in 90% of these indeterminates. Hepatitis C virus RNA detection was positive in 96% of antibody-positive cases tested, in 20% of patients who were already anti-HCV negative before transplantation and also demonstrated persistence of HCV infection in all cases who, being antibody positive prior to transplantation, lost these antibodies during follow up (9% of transplanted patients). In conclusion, hepatitis C virus infection is extremely prevalent in renal transplant recipients from Spain and is the main cause of chronic liver disease in these patients. Confirmation by supplemental assays of anti-HCV antibodies is not necessary, but hepatitis C virus RNA testing is indispensable to detect those cases who lose or do not develop hepatitis C virus antibodies.

Adult↗
PubMed1995

[Prevalence and clinical significance of autoantibodies in chronic hepatitis C treated with interferon alfa].

Autoantibodies against non organ-specific may be induced by IFN treatment in HCV liver chronic disease. In order to assess this issue and its clinical significance, 44 patients with chronic hepatitis C (anti HCV and RNA-HCV +) treated with IFN were evaluated. In 13 patients (29.5%) the following autoantibodies were detected during the treatment: AAML (5), AAN (9), ALK-M (4). Only in the A-LKM + patients there was a slight increase in aminotransferases with a reappearance of the viremia. Moreover 2 of these patients showed a flare of their liver disease without systemic findings. During the follow up (28) months one of the patients normalized the aminotransferases with clearance of serum HCV RNA. There were no statistical differences in the sustained response rate between patients with (2/13) or without (4/31) autoantibodies. The development of AAN, AAML and ALKM at low titer during IFN treatment in CH-C is without clinical relevance. However, ALKM detection is occasionally associated with the "breakthrough" phenomenon with HCV eradication, suggesting an immuno-modulatory activity of IFN.

Autoantibodies↗
PubMed1995

Does the healthy hepatitis C virus carrier state really exist? An analysis using polymerase chain reaction.

To determine whether the presence of hepatitis C virus (HCV) viremia correlates with the severity of liver disease in anti-HCV-positive apparently healthy blood donors, we studied 98 blood donors found positive for anti-HCV using enzyme-linked immunosorbent assay (ELISA). Each subject underwent a liver biopsy, a test for HCV RNA in the serum by polymerase chain reaction (PCR), and a panel of liver injury tests. As a result, 97% of the anti-HCV-positive blood donors had some type of histological abnormality:22 (22%) had minimal changes, 1 (1%) had chronic lobular hepatitis, 40 (41%) had chronic persistent hepatitis (CPH), and 32 (33%) had chronic active hepatitis (CAH). Only 3 subjects had a normal liver histology. HCV RNA was detectable in the serum in 65% of the anti-HCV-positive donors. HCV RNA in serum was detectable in none of the donors with a normal liver histology, in 36% (confidence interval [CI], 17% to 59%) of those with minimal changes, in 70% (CI, 53% to 83%) of those with CPH, and in 87% (CI, 71% to 96%) of those with CAH (P = .00001). HCV RNA was detectable in 75% of the donors with elevated (> 45 U/L) alanine transaminase (ALT) values and in 59% of those with normal ALT levels (P = not significant). The incidence of chronic hepatitis was higher in HCV RNA-positive than in HCV RNA-negative donors (88% vs. 50%; P = .00005).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1995

Genome detection in liver and peripheral blood mononuclear cells: predictor factors of sustained response in patients with chronic hepatitis C.

BACKGROUND: In chronic hepatitis C, relapses of liver disease occur in as many as 50% of patients responding to interferon (IFN) therapy. Although the presence of serum hepatitis C virus (HCV) RNA at the end of therapy predicts a relapse, its absence is not a reliable indicator of cure. In this study we have determined whether responders to IFN (normalization of liver chemistry and clearance of serum HCV-RNA) harbour HCV-RNA in the liver and peripheral blood mononuclear cells (PBMCs), and whether finding the genome at these sites has prognostic significance. METHODS: After the conclusion of therapy, we tested for HCV-RNA in the liver of all the patients (anti-HCV-positive): 16 complete responders with normalization of liver chemistry and clearance of serum HCV-RNA and five non-responders. In 13 of the 16 complete responders we also tested for HCV-RNA in PBMCs. Patients were followed up for 9 months. RESULTS: Liver HCV-RNA was detected in each of the five non-responders and in four of the 16 complete responders (25%). The viral genome was detected in the PBMCs of six complete responders (46%). During follow-up a relapse of hepatitis C occurred in the 10 complete responders with liver or PBMC HCV-RNA but in only one (16%) of the six complete responders without HCV-RNA in liver or PBMCs. CONCLUSION: Reliable information on the prognosis of chronic hepatitis C responders can only be obtained by testing for HCV-RNA in serum, liver and PBMCs at the end of therapy. Patients with HCV-RNA at any of these sites stand a high risk of disease relapse. The risk is low but not abolished in patients without detectable HCV-RNA.

Aged↗
PubMed1995

Pathophysiology of hepatitis C virus infection.

Any physiopathological hypothesis of HCV infection must explain at least some of the most characteristic features of this infection, namely: 1) persistent infection in the majority of exposed individuals, associated with a wide spectrum of histological damages and fluctuating biochemical expression; 2) low tendency towards spontaneous recovery; 3) high tendency to progress to cirrhosis; 4) high prevalence of autoimmune phenomenons; 5) very weak or absent immunity to reinfection. A model is given taking into account the nature of the HCV genome, the immune response of the host to the infection and the potential mechanisms(s) involved in viral persistence.

Antibody Formation↗
PubMed1992

[Psoas abscess: diagnostic and therapeutic usefulness of echography and computerized tomography].

BACKGROUND: Psoas abscess (PA) is a clinically infrequent entity. The abscess may form spontaneously (primary PA) or as a complication of contiguous infection (secondary PA). The use of ultrasonography (US) and computerized tomography (CT) facilitates diagnosis and treatment of a disorder which previously required surgery. METHODS: Nineteen cases of PA diagnosed over the last 7 years were retrospectively studied. Confirmation of diagnosis was established by exteriorization of pus with US, CT or during surgery. RESULTS: Three primary PA (16%) and 16 secondary PA (84%) were diagnosed. The foci of origin of the secondary PA were: urologic (50%), rachydeal (25%), gastrointestinal (12.5%) and iatrogenic lumbar infection (12.5%). The most frequent germs in the primary PA were: Staphylococcus aureus (67%) and in the secondary PA, enterobacteriae (50%). The diagnostic profitability of US was 41% (7/17) and for CT was 100% (15/15). Percutaneous drainage was performed in 9 patients which failed in 2 cases due to compactness of pus (22%) and in another 2 because of undiagnosed osteomyelitis (22%). Two patients (10%) with underlying disease died despite adequate medical-surgical treatment. CONCLUSIONS: Psoas abscess were secondary in 84% of the patients studied with most being due to enterobacteriae. The diagnostic profitability of computerized tomography was greater than that of ultrasonography (100% vs 41%). Percutaneous drainage is a valid therapeutic alternative. Relapse observed in this study was due to previously undiagnosed osteomyelitis.

Adolescent↗
PubMedDate not supplied

[Cytomegalovirus nephrotic syndrome].

The case of a 5 months old infant with a nephrotic syndrome after neonatal cytomegalovirus infection is reported. Genomic amplification nested-PCR for CMV was positive in renal biopsy. Treatment with gancyclovir was effective to maintain nephrotic syndrome remission. We stresses the importance to discharge an infections cause of the nephrotic syndrome of newborns and infants due to the possibility on curative treatment.

Antibodies, Viral↗
PubMedDate not supplied

[Prediction factors for lymph node involvement in carcinoma of the larynx].

A series of 60 patients with squamous cell carcinoma of the larynx who underwent surgery of the primary tumor and elective bilateral neck dissection were reviewed to determine the importance of certain clinical and pathological features as risk factors for lymph node metastasis. Tumor location, extension to the vallecula and to the pyriform sinus, T stage, histological grade, palpable lymph nodes, laryngeal motility, and macroscopic aspects were studied. Logistic regression analysis demonstrated that T stage, tumor location, and palpable lymph nodes were the most important predictors of lymph node metastases.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

[Correlation of lymph node status with survival in cancer of the larynx].

A histopathological study of regional lymph nodes was made in 50 patients with squamous cell carcinoma of the larynx in order to evaluate their prognostic significance for survival. Factors related with poor prognosis were presence and number of lymph node metastases and capsule rupture. The presence and grade of sinus histiocytosis was related with survival. In multivariate analysis, the presence of five or more metastatic nodes had an independent influence on survival.

Adult↗