Biomedical subjects
J Cabello
Publications and source records attributed to J Cabello.
[Neurocysticercosis: report of three cases and recommendations of treatment].
Neurocysticercosis is a pathology that we are finding nowadays over the immigrant population. It is produced by the Taenia solium parasite when it encyst into the central nervous system. The most frequent clinic is the focal crisis though it can come up as a general crisis; hydrocephalus, intracranial hypertension and even encephalitis can also happen. Its diagnosis is mainly based on imaging methods. The treatment is still controversial and must be individualized. The indication of antiparasitic drugs like albendazole and praziquantel has to be considered depending on the viability of the parasite, the number of cysts and their localization. If a cyst grows up the treatment is always recommended. In the case of an intracranial hypertension arises, this has to be treated before initiating any other action against the parasitic disease. Antiepileptic drugs are necessary to treat the symptoms. In some cases it is also recommended the use of steroids before and during the administration of antiparasitic therapy and even independently on their own.
[Spondylodiscitis and psoas abscess caused by Escherichia coli].
Explore the source record for details and available documents.
Non-conventional yeasts as hosts for heterologous protein production.
Yeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces cerevisiae has usually been the yeast of choice, but an increasing number of alternative non-Saccharomyces yeasts has now become accessible for modern molecular genetics techniques. Some of them exhibit certain favourable traits such as high-level secretion or very strong and tightly regulated promoters, offering significant advantages over traditional bakers' yeast. In the present work, the current status of Kluyveromyces lactis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris (the best-known alternative yeast systems) is reviewed. The advantages and limitations of these systems are discussed in relation to S. cerevisiae.
[Evidence-based medicine].
This paper presents the origins and the concepts in which the paradigm of a new line of clinical reasoning "Evidence Based Medicine", is based. We describe its basic components such as: problem-oriented clinical policy formulation; information retrieval distillation and synthesis of the medical literature; critical appraisal of the evidence and clinical application. Additionally, we describe them already well established, for promoting and Evidence Based Clinical Practice. We particularly focus on the so called "Cochrane Collaboration", an international initiative whose mission is to prepare, maintain and disseminate systematic and updated reviews of the effects of health care.
[The diagnosis of sleep apnea syndrome with a portable device. Our experience with 100 cases].
UNLABELLED: Portable devices might be an alternative method to diagnose patients suspected for obstructive sleep apnea syndrome (OSAS). We have analized one hundred patients consecutively referred to our especialized out-patient clinic for sleep breathing disorders. We have used a previously validated portable device-Polygraphics CNS-which records thoracoabdominal movement, nasobuccal airflow, ECG, oxymetry, body position, and continuous positive airway pressure (CPAP). Sixty patients showed an apnea/hypopnea index (AHI) > 15. Seven patients showed an AHI > 10f plus symptoms suggestive of OSAS. Two patients had an AHI between 5-10 and very high suspicion for OSAS; a subsequent CPAP treatment showed the disappearance of respiratory events and an evident improvement in oxymetric records. Twenty two patients showed an AHI < 5. Nine patients could not be classified and other diagnostic method were deemed necessary. CONCLUSION: A previously validated cardiorrespiratory portable polygraphic device was useful in most cases for taking diagnostic decisions in OSAS.
Sequence and functional analysis of the Streptomyces phaeochromogenes plasmid pJV1 reveals a modular organization of Streptomyces plasmids that replicate by rolling circle.
pJV1 is an 11 kb, high-copy-number conjugative Streptomyces phaeochromogenes plasmid that replicates by the rolling circle mechanism (RCR). Sequencing combined with functional analysis of deletion, insertion and frameshift mutations was used to characterize the genes involved in plasmid transfer and chromosome mobilization (Cma), the single-strand origin for RCR and an associated strong incompatibility (Sti) determinant. pJV1 contains two essential transfer genes whose expression is regulated by an adjacent repressor gene with similarity to the GntR family of regulators. A consensus sequence specific for the helix-turn-helix motifs of repressor proteins of Streptomyces plasmids is proposed. Unregulated expression of the transfer genes by inactivation of the repressor is lethal. Three additional genes increase intramycelial plasmid spread resulting in pock formation but, unlike the essential transfer genes, are not required for Cma. The pJV1 transfer genes and their regulatory region, but not the minimal replication region encoding the double-strand replication origin and replication protein, are similar in their sequence and arrangement to those of the Streptomyces nigrifaciens plasmid pSN22, revealing a modular organization of Streptomyces RCR plasmids.
Cocaine exposure induces changes in the ganglioside content of rat liver.
During cocaine exposure, the liver undergoes significant morphological and biochemical changes. We report here changes in the ganglioside pattern of rat liver after repeated administration (over 5 hours, one injection per hour) of a moderate dose of cocaine (10 mg/kg body weight). Cocaine exposure results in an accumulation of more complex gangliosides (GM1, GD1a, GD1b, GT1b and GQ1b) and a reduction of precursors (GM3, GM2, GD3 and GD2). Our results suggest that ganglioside biosynthesis could be affected by an alteration of vesicular transport from cis- to trans-Golgi cisternae produced either by cocaine itself or by some product of cocaine metabolism.
[Cardiac tamponade as the first manifestation of a pulmonary neoplasm].
Explore the source record for details and available documents.
[Evaluation of transparietal hepatic cholangiography in the diagnosis of cholestatic syndrome].
Three hundred and thirty-two percutaneous cholangiograms were done in 313 patients. The etiology was benign in 137 cases and malignant in 152 instances. The level of obstruction was correctly localized in 97.8% of cases. The total sensitivity was 88.5% and specificity 95.9%. Positive predictive value 95.4% and negative predictive value 89.6%. Major complications occurred in 3.3% of cases. Complications occurred more frequently when the bile ducts were dilated. They were not related to the number of attempts to puncture ther biliary tree or the experience of the physician.
[Alveolar hemorrhage].
Explore the source record for details and available documents.
[Adenocarcinoma of the cardia: results of distal esophagectomy and total enlarged gastrectomy using left thoracophrenolaparotomy].
An analysis was made of the results obtained in terms of postoperative complications, mortality and the 5-year survival rate in a series of 23 patients diagnosed as adenocarcinoma of the cardias who underwent total gastrectomy with distal esophagectomy via left thoracophrenolaparotomy, with esophagojejunostomy on a Roux-en-Y loop. We consider the results as favorable and conclude that this procedure has an application in adequately selected patients.
[Complications of acute gastroenteritis caused by Salmonella no typhi].
A review was made of the histories of 125 adult patients admitted for acute gastroenteritis (GEA) due to Salmonella no typhi. The complications that appeared in the series are analyzed. A total of 16 patients (12.8%) presented complications: the most common was bacteremia, 3 had renal tubular necrosis, 2 alithiasic cholecystitis that required surgery, 2 toxic megacolon, 2 rectal hemorrhage, 1 erythema nodosum and 1 intestinal perforation. The authors review the features of each complication.
[Results of extended resections in colorectal cancer].
We present 50 cases of super-radical surgery in cancer of the colon and rectum with tumoral spread. In the same period of time a total of 426 colorectal neoplasms were operated on, which represents 11% of the total. The mean age was 65.7 years, with a predominance of females (29/21). In every case the primitive tumor and contiguous organ affected were excised with an oncologic criterion; the principal location was the rectum, with 14 cases, and the viscus most affected was the female genital tract. The mean hospital stay was slightly longer than that of the general series (20.9/14); the mortality was 4%, a figure similar to that of the general series; the index of morbidity did not rise, survival was comparable and the patient's comfort was enhanced. In our opinion, this technique is indicated in all cases in which the tumor invades contiguous regions when local and/or general conditions permit.
[Immediate prognosis factors in total gastrectomy in cancer].
We present a series of 76 patients operated over a period of nine years, diagnosed as gastric cancer (CG), in whom radical oncologic total gastrectomy (GT) with esophago-jejunal anastomosis (EY) on a Roux-en-Y loop (Y-R) was performed. The tumor was located in the cardias in 27 patients, body in 37, fornix in 3, gastric stump in 7 and over plastic linitis in 2. We performed total gastrectomy enlarged to the spleen in 63 patients, to the pancreas in 28, to the colon in 5 and to the liver in 4. We describe the most salient technical details of the surgical intervention. Twenty-six percent of the patients presented postoperative complications, which we divided into two groups: anastomosis failures, among which are emphasized 4 severe esophagojejunal fistulas, a fistula of the duodenal stump (MD) and respiratory complications. The mortality was 7.8%, corresponding to 4 esophago-jejunal fistulas, 1 fistula of the duodenal stump and 1 bilateral bronchopneumonia. We analyzed various parameters that we consider important prognostic indicators of postoperative morbi-mortality. Applying the Fischer test, we attempted to assign statistical significance to these parameters. Age over 60 years increased postoperative mortality. Super-radical surgery did not increase mortality. On the other hand, patients in stages IIIb and IV have a worse prognosis, with a mortality in the first 30 days of 28.5%. Esophago-jejunal anastomosis on a Roux-en-Y loop is the procedure of choice used by our center after total gastrectomy for gastric cancer, yielding the most acceptable morbi-mortality figures.
[Non-excisional treatment of spontaneous rupture of the esophagus].
We present 7 personal cases of Boerhaave syndrome, 6 treated surgically and one managed with conservative measures. Boerhaave's syndrome is one of the most serious gastro-intestinal perforation pictures. Although its frequency is scant, because of its seriousness it is important to be aware of it for early diagnosis. While a prompt diagnosis is important in any pathology, it is even more so in this syndrome since there is a clear relationship between the time lapse from perforation to the onset of treatment and the rate of survival. Although we present one case cured medically, this is exceptional and treatment is eminently surgical and should be as early as possible. The technique that provides the best results and an excellent morbimortality rate in relation to efficacy is primary suture followed by a fundal patch. In delayed cases with patients in deteriorated condition, other techniques can be considered. Due to its initial severity and a tendency to postoperative complications, the patient should be closely controlled, and correct antibiotic therapy and complete parenteral support nutrition are very important in treatment.
[Pulmonary metastases as the presenting form of a malignant hemangiopericytoma].
Explore the source record for details and available documents.
[Psittacosis of non-avian origin].
Explore the source record for details and available documents.