Immunohistochemical demonstration of human and murine pregnancy-associated serum proteins in maternal and placental tissue.
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Biomedical subjects
J Chemnitz
Publications and source records attributed to J Chemnitz.
Repair in arterial tissue.
Both reendothelialized and non-reendothelialized surfaces of rabbit aortas, subjected to an embolectomy catheter lesion, were sectioned parallel to the neointimal surface. Microfilament bundles within endothelial cells were associated with extracellular anchoring filaments, lying parallel to the long axis in sections from reendothelialized areas. Proteoglycans coated the surface of anchoring filaments and joined together all the elements of connective tissue in the subendothelial space. In non-reendothelialized areas covered by smooth muscle cells, anchoring filaments were less prominent, almost no proteolglycans were visible, and connective tissue components were randomly mixed. We propose an influence of endothelial cells on the orientation and composition of the subendothelial connective tissue.
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Ultrastructural characterization of a mouse melanoma cell line, a mouse fibroblastic cell line, and hybrids between them.
This report describes a transmission electron microscopic study on hybrids between subhexaploid mouse melanoma cells and subtetraploid mouse fibroblasts. The melanoma cell line was heterogeneous in terms of pigment production, but all cells contained melanosomes, although in different stages of development. Characteristic features of the fibroblasts included the cytoskeleton, endocytic vesicles, and occurrence of dilated cisternae of granular endoplasmic reticulum. Despite the gene dosage in favour of the melanoma parent cell, the hybrids were devoid of melanosomes, and their phenotype was typically fibroblastic in character.
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Formation of large vacuoles in tumour cells grown in suspension.
In suspension cultures of JB-1-E cells approximately 11% of the cells contained a single large vacuole ("balloon cells") or signet-ring cells). Such cells were rarely observed in monolayer cultures, where the cells were attached to a substrate. Only a few "balloon cells" were observed in suspension cultures of HeLa S3 cells. Both cell lines were able to phagocytize latex particles of 5.7 micrometer mean diameter. Nocodazole significantly reduced the formation of "balloon cells" in suspension cultures of JB-1-E cells. Transmission electron microscopy indicated that various cytoplasmic membrane components probably could contribute to the membrane material of the vacuoles. It is believed that the formation of "balloon cells" is due to changes in the regulation of the cellular membrane material.
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The effect of cooling rate during perfusion on function and morphology of rabbit kidney grafts.
The effect of cooling of rabbit kidneys during the initial flush with Collins' solution for simple hypothermic storage was studied. After cooling at an average rate of 3.7 degrees C/min. graft function was found to be immediately life-sustaining, and renal morphology after perfusion as well as 30 min. and 24 hours posttransplant was found to be normal. Posttransplant function after cooling at a rate of 7.2 degrees C/min. was significantly lower, permitting survival of only 30% of recipient animals, and damage was seen in proximal tubular cells.
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Repair in arterial tissue. Endothelial regrowth, subendothelial tissue changes and permeability in the healing rabbit thoracic aorta.
The role of the endothelium and subendothelial connective tissue in the permeability of a healing intima was studied by vital staining with Evans blue and transmission electron microscopy after severe mechanical lesion of the rabbit aorta. Reendothelialization, decreasing permeability, and organization of neointimal connective tissue were concomitant events in the healing processes. The determinant factor in decreasing permeability was reendothelialization with the formation of endothelial flaps and junctions. Changes in the subendothelial connective tissue seemed also to be factors that influenced the permeability.
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Repair in arterial tissue. 2. Connective tissue changes following an embolectomy catheter lesion. The importance of the endothelial cells to repair and regeneration.
The neointimal hyperplasia following a severe mechanical lesion of the rabbit thoracic aorta was studied by vital staining with Evans blue and transmission electron microscopy. Neointimal tissue covered with endothelium contained organized laminated elastin-rich commective tissue. On the contrary neointimal connective tissue covered with pseudoendothelium was disorganized, with a tendency to fibrosis. Reendothelialization, re-establishment of intimal barrier function and formation of lamellated neointimal connective were parallel events. The importance of a intact subendothelial zone controlling healing processes is discussed. Interaction of endothelium and smooth muscle cells seems to be essential in the regulation of neointimal tissue formation and is probably implicated in a general vascular reactive pattern.
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Tumor cell interactions in vitro microtubules, 100 angstrom filaments, and contractile microfilaments of tumor cells involved in "emperipolesis".
An increase in the number of examples of emperiopolesis, i.e., cells within cells, was observed subsequently to addition of Bt2cAMP to spinner flask cultures of JB-1-E tumor cells. A conspicuous arrangement of microtubules and 100 angstrom filaments in the tumor cells involved in emperiopelesis was observed. Absence of emperipolesis in cultures treated with cytochalasin B indecates a possible role of the contractile microfilaments in the events leading to emperiopolesis. The significance of and the term emperipolesis are discussed.
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Tumour cell interactions in vitro. Evidence for the occurrence of tumour cells within other tumour cells.
After addition of dibutyryl cAMP to spinner flask cultures of JB-1-E tumour cells an increase in the number of examples of emperipolesis was observed. Ruthenium red staining of the cells revealed a conspicuous staining of the cell membrane of the outer cell, while that of the inner cell was unstained. The differences in staining properties of the cell membranes of the outer and inner cells involved in emperipolesis and apparently viable inner cells make it evident that coexistence of cells--one within another--is possible.
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Microtubule reassembly-interactions between microtubules and pore complexes, endoplasmic reticulum and 100 A filaments in tumour cells in vitro.
100 A filaments and annulate lamellae were often observed in the vicinity of vinblastine-induced paracrystals in tumour cells treated with nocodazole. There was often a direct continuity between 100 A filaments and paracrystals. The assembly of microtubules was examined in nocodazole-treated cells with special attention to possible initiating sites. We found an apparent association of microtubules with pore like structures associated with the endoplasmic reticulum. A complex system of 100 A filaments, microtubules and endoplasmic reticulum was observed. The significance of these results is discussed.
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Interrelationship between annulate lamellae and the cytoplasmic microtubule complex in tumor cells in vivo and in vitro.
Annulate lamellae were observed in untreated in vivo ascites tumor cells with a diminished cytoplasmic microtubule complex. The ascites tumor cells in vitro responded to prolonged colchicine treatment with the formation of annulate lamellae. Simultaneous treatment with dibutyryl cycle adenosine monophosphate and colchicine seemed to enhance the formation of annulate lamellae. Single pore complexes were found in the granular endoplasmic reticulum in untreated tumor cell in vitro, and a close association of microtubules with the nuclear envelope was observed. Our results suggest the existence of an interrelationship between the cytoplasmic microtubule complex and certain other cell structures, i.e. the nuclear envelope, annulate lamellae, and single pore complexes.
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En face organ cultures of rabbit aortic segments after a single dilatation trauma in vivo. A new model in the study of endothelial regeneration.
Following an in vivo lesion of the rabbit thoracic aorta, endothelial regeneration was studied in silver-stained specimens from en face organ cultures of aortic segments with and without intercostal arteries. The in vivo lesion had been inflicted by an embolectomy catheter. Our results confirmed that endothelium regenerates from preexisting endothelium around the mounths of the intercostal arteries. A conspicuous observation was the orientation pattern of endothelial cells during regeneration. Transmission electron microscopy suggests that the cell cytoskeleton plays a role in the regulation of regeneration. The organ culture technique described, complementary to in vivo and in vitro investigations with cell cultures, seems to be a valuable tool in further investigations on factors involved in the repair of arterial tissue.
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Observations on the association of annulate lamellae with vinblastine-induced paracrystals in tumour cells in vitro.
Tumour cells in vitro responded to prolonged treatment with colchicine with the formation of annulate lamellae. Following the colchicine treatment, paracrystals were induced with vinblastine. A close association of annulate lamellae with vinblastine-induced crystals was observed. The hypothesis that annulate lamellae and the related unclear membrane with the pore complexes may play a role in the control of the cytoplasmic microtubule complex is discussed.
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Repair in arterial tissue. The role of endothelium in the permeability of a healing intimal surface. Vital staining with Evans blue and silver-staining of the aortic intima after a single dilatation trauma.
The role of the endothelium as a factor determining the permeability of a healing aortic intima after a severe mechanical lesion in the rabbit was studied by vital staining with Evans blue, by surface light microscopy after silver-staining, fluorescence microscopy and by other methods. Endothelium to develop from pre-existing endothelium in the mouths of intercostal arteries and at the borders of the lesion, remained unstained by vital staining, whereas denuded surfaces as well as pseudo-endothelium, probably derived from smooth muscle cells in the neointima, were intensely coloured. There was a general tendency towards a decreasing permeability of the aortic wall throughout an experimental period of 6 months. The determinant factor in the decreasing permeability was the presence of genuine endothelium, but changes in the endothelium, probably in the form of differentiation of intercellular junctions and changes in the connective tissue in the deeper layers, seemed to be additional factors which influenced the permeability.
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Repair in arterial tissue. Electron microscopy of Evans blue vital stained embolectomy catheter lesion of the rabbit thoracic aorta.
The rabbit thoracic aorta was studied by EM and vital staining with Evans blue at vaired points of time after a single lesion produced by an embolectomy catheter. EM of white areas: the surface cells of the myo-intimal thickening resembled endothelial cells with a discrete occurrence of microfilaments inside the plasma-membranes and rather differentiated flap-like junctions. EM of blue areas: the surface cells resembled modified smooth muscle cells with a heavily contrasted zone just inside the luminal plasma membrane containing closely packed microfilaments. The cell contacts either were missing, or they presented undifferentiated side-to-side contacts.
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Increasing occurence of tumour cell -- tumour cell emperipolesis in the regenerating JB-1 ascites tumour.
During the investigation of JB-1 ascites tumour cells in recurrent growth several cases of emperiopolesis were observed. Different types of emperipolesis are presented. Light microscopical serial sections, autoradiography and transmission electron microscopy indicate that the cells involved in emperipolesis are separate cells, and the authors suggest that cytopagocytosis is the initial mechanism in the development of emperipolesis.
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Light and electron microscopy of the JB-1 ascites tumor at different stages of growth.
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Tumour cell-tumour cell emperipolesis studied by transmission electron microscopy.
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