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J Chmielewska

Publications and source records attributed to J Chmielewska.

34 records · Page 2Linked to original sources
PubMed1982

Experience with chromogenic substrates in studies of clotting and fibrinolytic systems.

Experiences in the application of chromogenic substrates for the evaluation of clotting variables are reported. The use of a special buffer system enables the determination of factor Xa by the thrombin substrate Chromozym TH. Prothrombin in plasma can be determined using the substrate S-2238 after activation with factor Xa or Ecarin. Factor VII is activated with thromboplastin and is estimated indirectly via factor Xa activity. Comparison of the amidolytic method for factor VII with prothrombin times of patients on oral anticoagulants shows good correlation. Chromogenic substrates can be also used for determining platelet factor 3 (S-2238), plasminogen and alpha 2-antiplasmin.

Blood Coagulation↗
PubMed1981

An assessment of an amidolytic assay for factor VII in the laboratory control of oral anticoagulants.

A comparison has been made between the prothrombin time test using British Comparative Thromboplastin (BCT) and a chromogenic substrate assay for factor VII in the assessment of laboratory control of oral anticoagulants in short-term and long-term patients. Opportunity was also taken to compare the findings with parallel results obtained with the venous Thrombotest technique and a specific clotting assay for factor VII. There was good agreement between the amidolytic factor VII assay, using a method modified from Seligsohn et al (1978) with the Quick test using BCT and Thrombotest in 60 long-term patients. Tests in 53 patients within the first 3 weeks of starting oral anticoagulant administration gave less satisfactory agreement between the above amidolytic method and the conventional tests. In contrast, there was a good correlation between the two conventional tests in both groups and also between the clotting and amidolytic factor VII method. Although the results are an improvement on previous, less satisfactory correlations between the BCT prothrombin time method and amidolytic assays for factor II and X, the present study indicates the limitations of a specific clotting assay versus a broad spectrum extrinsic clotting test in oral anticoagulant control. While not warranting the routine use of the chromogenic assay for factor VII in place of the prothrombin time using BCT, the factor VII amidolytic assay offers a limited but dependable guide to dosage in long-term patients. The complexity of the technique in its present form militates against its adoption for routine anticoagulant control in hospital laboratories.

Acenocoumarol↗
PubMed1976

Contact with estrus female as a reward for instrumental response in a growing male rat from the 3rd up to the 14th week of life.

The bar-press response reinforced by the contact with estrus female was investigated in growing male rats. The bar-press latency as well as the subjects' behavior changed distinctly in the course of the study with the increase of experience and maturation of the subjects. Some behavioral items such as, nasogenital contact, climbing on the head, and running around showed gradual increase during succeeding weeks of life, parallelly to the decrease of the sitting under female, and crawling under female. A gradual shortening of the bar-press latencies was observed from the 4th to the 11th week of life succeeded by a prolongation from the 11th to the 14th week. On the basis of the changes in bar-press latencies as well as of the analysis of the subjects' behavior it is suggested that the young male rat between the 4th and 7th week of life comes under an increasing influence of sexual motivation.

Age Factors↗