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Biomedical subjects

J Cinátl

Publications and source records attributed to J Cinátl.

At least 19 recordsLinked to original sources
PubMed2002

[Antibodies to granulocytic Ehrlichiae in the population of the western and central part of the Czech Republic].

A total of 963 subjects in four groups from western and central Bohemia were examined in 1998-2000 for the presence of antibodies against granulocytic ehrlichiae. In the indirect fluorescence assay, a specific antigen from ehrlichiae (HGE) cultivated in-vitro and commercial anti-IgG and anti-IgM secondary antibodies were used. An overall prevalence of HGE antibodies of the class IgG corresponding to titres < or = 1/80 and 1/160 was found to be 21.0 and 13.5%, respectively, with a predominance during the warm season (May-October). The seasonal seroprevalence was discriminated best from an all-year background by titres 1/80-1/160. Elevated antibodies were found more frequently in older subjects; no effect of gender was observed. Antibodies of the class IgM were detected only exceptionally and did not exceed the titre of 1/40. Only minor differences were observed in the prevalence of HGE antibodies between groups differing geographically or by the presence of Borrelia antibodies. The seasonal incidence of HGE antibodies did not differ significantly from that of Borrelia antibodies.

Adolescent↗
PubMed1999

A dimeric mutant of human pancreatic ribonuclease with selective cytotoxicity toward malignant cells.

Monomeric human pancreatic RNase, devoid of any biological activity other than its RNA degrading ability, was engineered into a dimeric protein with a cytotoxic action on mouse and human tumor cells, but lacking any appreciable toxicity on mouse and human normal cells. This dimeric variant of human pancreas RNase selectively sensitizes to apoptotic death cells derived from a human thyroid tumor. Because of its selectivity for tumor cells, and because of its human origin, this protein represents a potentially very attractive, novel tool for anticancer therapy.

3T3 Cells↗
PubMed1998

Antitumor action of bovine seminal ribonuclease. Cytostatic effect on human melanoma and mouse seminoma.

This paper reports on the antitumor activity of BS RNase on human melanoma and mouse seminoma. Human melanoma cells established in culture were extremely susceptible to BS RNase, administered in concentrations ranging from 1-100 microg/ml. Concentrations of BS RNase over 10 microg/ml caused complete inhibition of cell growth. Bovine pancreatic ribonuclease (RNase A), a prototype of the ribonuclease superfamily, did not exert any effect under these conditions. Based on our previous results, athymic mice bearing human melanoma or mouse seminoma were treated with intratumoral administration of BS RNase (12.5 mg/kg b.w.). This dose was injected for five consecutive days excluding weekends. The intratumoral administration of BS RNase to nude mice bearing human melanoma showed a significant antitumor effect. There were no tumors seen in eighty percent of mice treated for three weeks, and tumors in the other mice diminished significantly. After some delay the tumors started to regrow. Prolonging of the treatment to five weeks had a similar effect. The effect of BS RNase on mouse seminoma was well pronounced. Five to seven doses of BS RNase were sufficient to eliminate tumors in all treated mice. However, as in the previous experiment, the growth of tumor tissue later reappeared.

Animals↗
PubMed1983

Immunological properties of lymphoblastoid cell lines (UHKT) - 1, 2, 3, 4, 5, 6) established from cases of human leukemias.

Immunological characteristics of stabilized cell lines (UHKT-1, 2, 3, 4, 5, 6) established from cells of peripheral blood of patients with leukemia were studied. Attention was focussed on the analysis of various membrane markers (e. g. T, Ia-like, antigens, membrane immunoglobulin, receptors for sheep erythrocytes, for IgG-Fc fragment, for the third component of the complement and expression of beta-2 microglobulin). The cells of these lines were also tested for the presence of Epstein-Barr virus-determined nuclear antigen (EBNA) and other EBV antigens. Chromosomal analysis was performed repeatedly. The UHKT cell lines were compared to the standard cell lines obtained from other laboratories. The results showed that rabbit antiB xenosera revealed Ia- antigen on the cells of B cell lines. The determination of T and B cell types using rosette test was less convincing in some lines.

Antigens, Surface↗
PubMed1982

Culture of macrophage cell lines from normal mouse bone marrow.

The use of semisolid medium for the culture and cloning of haematopoietic cells has helped our understanding of their proliferation and differentiation. It has been shown that mixed colonies of granulocytes and macrophages developed, in the presence of colony-stimulating factor (CSF), from their common precursor, granulocyte-macrophage colony-forming cells (GM-CFC). Attempts at recloning these colonies in semisolid medium suggested that granulocytes and macrophages were differentiated cells incapable of further proliferation. However, our studies on cultures of larger numbers of cells demonstrate that while this may be the case for granulocytes, macrophages seem to be capable of long-term proliferation.

Animals↗
PubMed1982

Inhibitory effects of 5-azapyrimidine nucleosides on cellular immunity.

The effect of 5-azacytidine (5-AzCR) and 5-aza-deoxycytidine (5-AzCdR) on the survival of skin grafts in mice and rats, the action of these drugs on regional GVH reaction, as well as the formation of haemopoietic colonies (CFU-5) in the spleen were studied. Both drugs prolonged the life span of skin grafts when administered 24 hr before transplantation, or on the 4th post-transplantation day. However, they were little effective when injected 24 hr after skin grafting, or after induction of the regional GVHR. Following intraperitoneal administration, they inhibited CFU-5 formation. Two-hour incubation in vitro of cells with 5-AzCR significantly reduced their GVH reactivity and capacity to form CFU-5; 5-AzCdR under the same conditions was ineffective.

Animals↗
PubMed1982

Effect of cold adaptation on total enzyme activities of L cells.

Using cytochemical methods, the authors tested enzymatic reactions in L-As and L-C3 cells. They found that esterase activity in particular, but also several other enzyme activities, were higher in L-C3 cells than in L-As cells. This furnished further evidence of the raised metabolic activity of the cold-resistant cell subline L-C3 compared with the L-As line.

Acclimatization↗
PubMed1982

Human lymphocyte culture in semi-solid medium.

We pointed out some sources of variations in the results obtained by the method of human peripheral lymphocyte culture in semi-solid medium. The influence of changes in five important parameters of the two-layer method was studied: agar concentration in the upper layer, cell concentration, mitogen dilution, type of culture medium and serum. We found that the optimal conditions for lymphocyte culture and for the evaluation of the results were 0.3% agar concentration in the upper layer, the concentration of 5 x 10(5) cells/ml, and a double dilution of phytohaemagglutinin. Of the culture media tested, MEM with 0.2% Bactotryptose supplemented with 20% inactivated mixed human serum proved most appropriate. Cells in lymphocyte colonies were characterized morphologically, cytochemically and by means of basic membrane features. The possibilities of using the method in clinical work are shown and the necessity of standardizing the culture technique is emphasized.

Agar↗
PubMed1981

Establishment and characterization of permanent cell lines from patients with acute and hairy cell leukemia.

Four cell lines were established from peripheral blood of patients with leukemia. All lines express the Epstein--Barr virus nuclear antigen (EBNA) and therefore should be classified as lymphoblastoid cell lines. However, one of the lines UHKT-5 established from a patient with acute myelomonocytic leukemia has some features not typical for lymphoblastoid cell lines. The cells resemble to macrophages with the phagocytic ability for yeasts, strong alpha-naphthylacetate esterase positivity in phagocytizing cells and unusually long villi. Another line UHKT-7 established from a patient with hairy cell leukemia expresses the same isotype of membrane immunoglobulins as the original hairy cells.

Adult↗
PubMed1981

Human placental conditioned medium (from different phase of pregnancy) as a source of colony-stimulating activity for cultures of human and mouse. granulocyte-macrophage colony-forming cells in semisolid agar.

We have investigated the effect of conditioned medium derived from cultures of human placental tissue from different phases of pregnancy on in vitro growth of human and mouse granulocyte-macrophage colony forming cells in semisolid agar. We have confirmed that the colony-stimulating activity of human placental conditioned (normal delivery) medium was equivalent to the activity of peripheral blood leucocyte underlayers when medium was added at a 5%-20% concentration to the semisolid agar. Placentas from the 12th amd 10th week of gestation were found not convenient for preparation of medium with high CSA; the activity of media prepared from their cultures was not significantly higher than the autostimulating activity of bone marrow alone. Human placental conditioned medium proved inconvenient for cultures of mouse GM-CFC in semisolid agar containing foetal calf serum.

Agar↗
PubMed1981

Cultivation and differentiation of haemopoietic CFU-C cells in semisolid medium.

The possibilities of cultivating murine bone marrow in semisolid agar and in methyl cellulose were investigated. The proliferative capacity of the haemopoietic CFU-C stem cells were demonstrated. The growth of CFU-C in fresh murine bone marrow and murine marrow short-term preserved at -75 degrees C was compared. The content of cells capable of proliferation was lower and the onset of colony formation was delayed in preserved bone marrow. Procedures appropriate for routine work were employed for evaluating haemopoietic colonies.

Agar↗
PubMed1980

Immunosuppressive effects of a synthetic polymer poly N-(2-hydroxypropyl)methacrylamide (Duxon).

Poly N-(2-hydroxypropyl)methacrylamide (Duxon) was studied as a substitute of blood plasma and was found to prolong significantly the survival of semiallogeneic skin grafts in mice and rats and to reduce MLC reactions of human lymphocytes. It did not inhibit the growth of lymphocytes and the growth of the fibroblast-like (diploid) or epithelioid (heteroploid) cell lines in tissue culture.

Animals↗