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J Clements

Publications and source records attributed to J Clements.

31 records · Page 2Linked to original sources
PubMed1986

Sequence analysis and transformation by the catabolic 3-dehydroquinase (QUTE) gene from Aspergillus nidulans.

The induction of catabolic 3-dehydroquinase by quinic acid in Aspergillus nidulans has been shown to involve transcriptional control and yields a single major 0.8 kb mRNA. The nucleotide sequence of the catabolic 3-dehydroquinase QUTE gene has been determined and contains a single uninterrupted open reading frame of 462 bases encoding a 16,505 Da protein of 153 residues. Comparison with the corresponding QA2 gene of Neurospora crassa reveals the absence of 75 nucleotides encoding 25 amino acids from the centre of the QUTE gene of A. nidulans and the presence of 21 additional nucleotides at its 3' end. There is no nucleotide or amino acid homology between these two elements. A 16 bp inverted repeat (5' GGCAGAGCGTTCTGCC) shows similarity to such repeats found in other fungal promoters. The functional integrity of the QUTE gene was demonstrated by the transformation of a qutE mutant strain which regains growth on quinic acid as sole carbon source. Four of the twelve transformed strains examined contained vector sequences integrated at the qutE locus, and these strains all exhibited normal regulation of 3-dehydroquinase even when 16 copies of the QUTE gene were present.

Amino Acid Sequence↗
PubMed1986

Sleep problems in handicapped children: a preliminary study.

Data are reported from an exploratory study looking at the prevalence of sleep problems (broken sleep and limited hours of sleep) in a population of handicapped children. Some degree of difficulty occurred in over a third of the population. There were strong associations with age, a range of serious daytime behavioural difficulties and indices of family stress. Some support for distinct subgroups (night waking, limited hours) was obtained. The implications of these findings are discussed.

Adolescent↗
PubMed1985

Structure of canine pulmonary surfactant apoprotein: cDNA and complete amino acid sequence.

The apoproteins of pulmonary surfactant (PSAP) are thought to be critical for normal surfactant function. They bind to surfactant phospholipids and enhance their ability to form surface films in vitro. These acidic glycoproteins have monomeric molecular weights of 36,000, 32,000, and 28,000 (PSAP-36, -32, and -28). Each member of this family of proteins has a similar amino acid composition and their differences in electrophoretic mobility are due in part to glycosylation. We have derived the full amino acid sequence of PSAP-32 from the nucleotide sequence of PSAP cDNA. A cDNA library was prepared from canine lung poly(A)+ RNA and screened with oligonucleotide probes that were based on the NH2-terminal amino acids of PSAP-32 determined by Edman degradation. This protein has the striking feature of collagen-like and non-collagen-like sequences in the same polypeptide chain. There are 24 Gly-Xaa-Yaa triplets, where Yaa is often hydroxyproline. These repeats comprise one-third of PSAP near the NH2 terminus. The remaining two-thirds of PSAP is resistant to bacterial collagenase digestion and contains a possible N-glycosylation site near the carboxyl terminus. The NH2-terminal one-third of PSAP-32 probably contains the cysteine involved in interchain disulfide bonds.

Amino Acid Sequence↗
PubMed1985

Psychologic factors in low-back-pain disability.

UNLABELLED: Biomechanical tests, physical examination, spinal radiographs, a modified Minnesota Multiphasic Personality Inventory (MMPI), and a psychologic health inventory derived from the Hopkins Symptom Checklist were applied in 321 randomly selected men, ages 18-55. Each subject was evaluated for prior and current low-back pain (LBP) complaints by use of a modification of the McGill pain questionnaire. Subjects were then categorized as having no LBP (n = 106, 33%), moderate LBP (n = 144, 44.8%), and severe LBP (n = 71, 22.1%). Subjects were further subcategorized as not disabled (defined as equal or less than seven days of work lost in the previous year) and disabled (work loss greater than seven days in the prior year). RESULTS: cumulative scores of the Hopkins Checklist showed that a mean score for no LBP equaled 2.5 +/- 2.9; moderate LBP was 4.3 +/- 3.2; and severe LBP was 4.6 +/- 3 (p = .0000). The disabled group was significantly different on both MMPI and Hopkins Checklist. The 20 disabled subjects had significant elevations of hypochondriasis (p = .0006) and hysteria (p = .005) when compared with nondisabled subjects with LBP. The mean score for disabled subjects on the Hopkins Checklist was 7.35 +/- 4.49, while the score was 4.17 +/- 3.15 (p = .0001) for the nondisabled subjects with LBP. In the general male population, patients with disabling LBP conditions form a distinctive subgroup comparable to those patients seen in selected LBP clinics.

Adult↗
PubMed1979

Leucomalachite green assay for free hemoglobin in serum.

A new colorimetric assay for the determination of serum free hemoglobin utilizing the noncarcinogenic compound leucomalachite green is described. The reaction is complete in 8 min, with the resulting color stable for at least 30 min. The absorption maximum for the leucomalachite green reaction product was observed at 617 nm. Precision studies at 50 and 300 mg/l free hemoglobin concentrations resulted in within-run variations of 1.9 and 1.7%, while the day-to-day variations were 6.9 and 4.0%, respectively. Accuracy studies at concentrations of 50, 150 and 300 mg/l resulted in recoveries of 88, 95 and 98% of theoretical values.

Aniline Compounds↗
PubMed1975

Comparison of lipids in total brain tissue from five mouse genotypes.

Brain tissue from adult male and female mice of the C57BL/6J, C57BL/6J-AW-J, BALB/cJ, SJL/J, and DBA/2J genotypes was examined for brain weight, total protein, total lipid, cholesterol, phospholipid, plasmalogen, sulfatide, nonganglioside-glycolipid sphingosine, and ganglioside N-acetyl neuraminic acid, fatty acid, and sphingosine. No significant differences were found between sexes for any of these constituents. When compared to the overall average obtained for other animals, the DBA/2J, C57BL/6J-AW-J, and BALB/cJ mice contained lower quantities of plasmalogen and sulfatide compared to the overall averages obtained for the other genotypes. In addition, the sterol content in DBA/2J mice was significantly higher than the overall average value obtained for the other animals.

Animals↗
PubMedDate not supplied

Somatic recombination and mutation assays in Drosophila: a comparison of the response of two different strains to four mutagens.

To compare the responses of 2 different strains used in somatic mutation assays of Drosophila, 4 chemical mutagens - methyl methanesulfonate, ethyl nitrosourea, adriamycin and daunomycin - were tested under standardized conditions in parallel experiments. Positive results were obtained for all 4 chemicals in both strains but there was a marked difference in the yield of somatic mutations between the 2 strains.

Animals↗
PubMedDate not supplied

Demonstration of acute post-viral myelitis with magnetic resonance imaging.

Post-infectious transverse myelitis is an important clinical entity in childhood, but the diagnosis is difficult to confirm by conventional radiologic investigations. We report a patient with acute, cervical, post-infectious transverse myelitis whose diagnosis had been established by the distribution and evolution of lesions on serial magnetic resonance imaging (MRI). Autopsy results confirmed the diagnosis of post-infectious transverse myelitis and the presence of MRI-identified lesions. To our knowledge, this patient is the first child reported with MRI findings in post-infectious transverse myelitis and suggests that MRI may be valuable in the diagnosis and management of children with this syndrome.

Acute Disease↗