Biomedical subjects
J Conde
Publications and source records attributed to J Conde.
Misreporting of maternal mortality in Puerto Rico.
Maternal mortality (MM) continues to be a problem that plagues many developed and underdeveloped countries around the world. It has been estimated that the minimum amount of underreporting in the U.S. to be 20%, resulting in MM rates that may be substantially higher than reported. The national goal for the MM for the year 1990 has been set at 5/100,000, and at the present trend it is expected that this may be achieved among the white population, but not among minorities. P.R. reported a maternal mortality rate of 5/100,000 in 1975. It was suspected that such a low rate was due to underreporting, a study was undertaken to investigate that possibility. The results indicated that there was severe underreporting of maternal deaths during 1978 = 79. Recently, there has been a growing concern that the level of underreporting in PR continues to be high. Since there has been no evidence that the surveillance has improved, the Dept. of Health requested from the Dept. of Ob-Gyn of the University of Puerto Rico Medical School and the Dept. of Maternal and Child Health to conduct a study to find out if the previous findings held true for recent years. The study was based on the review of selected medical records corresponding to deaths of women of childbearing age whose causes of death, as coded in the death certificate, were considered as having a high probability of masking a misreported maternal death. It was decided to investigate those deaths occurring in 1982, to see if the results of the previous study had caused any impact on the surveillance of maternal deaths in Puerto Rico.
Congenital bilateral posterolateral and anterior diaphragmatic defects.
The occurrence of bilateral posterolateral and anterior diaphragmatic congenital defects has not been previously reported. We present a case with associated abnormalities including pulmonary hypoplasia and interventricular septal defect.
Saccharomyces cerevisiae mutant defective in exo-1,3-beta-glucanase production.
Saccharomyces cerevisiae S288C produced two laminarinases (1,3-beta-glucanases) which were separated by diethylaminoethyl-Sephadex column chromatography; one was an endo-1,3-beta-glucanase, and the other was an exo-1,3-beta-glucanase active not only on laminarin but also on pustulan (1,6-beta-glucan) and on p-nitrophenyl-beta-D-glucoside. A mutant defective in the production of this last enzyme was isolated, and the mutation was named exb1-1. The selection procedure was based on the capacity of exo-1,3-beta-glucanase to hydrolyze synthetic glucosides. The level of endo-1,3-beta-glucanase in cell extracts of the mutant was normal, but the exo-1,3-beta-glucanase could not be detected by column chromatographic analysis of these extracts. The mutant phenotype, recessive in heterozygous diploids, was stable through successive meioses and showed a Mendelian segregation, indicating that the mutation affected a single gene, which was named EXB1. The lack of production of exo-1,3-beta-glucanase persisted through all the phases of growth, but growth itself was not impaired by the enzyme deficiency.
[Gorlin's syndrome. Two case presentations].
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A mutant of Saccharomyces cerevisiae defective for nuclear fusion.
A mutant unable to fuse nuclei during mating has been isolated from standard wild-type Saccharomyces cerevisiae. Tetrad analysis of the mutation responsible for this defect (kar1-1) shows that it segregates as a single Mendelian factor. The defect kn kaf1-1 appears to be nuclear limited. Cytological and genetic evidence shows that in this mutant the events associated with zygote formation are normal until the point of nuclear fusion. The consequence of this defect is the formation of a multinucleate zygote which in subsequent divisions can segregate heterokaryons and haploid heterplasmons.
Plasma kallikrein amidolytic activity in bronchial asthma.
We have investigated plasma kallikrein amidolytic activity in the following groups of patients: 1. Normal control group of blood donors. 2. Extrinsic pollen-activated bronchial asthma patients, during periods of symptomatology and at a later time after the spring. 3. Subjects with atopic bronchial asthma in acute phase when admitted to our hospital's emergency room and later when clinically recovered. 4. Subjects with extrinsic bronchial asthma, sensitive to Dermatophagoides pteronyssinus and Dermatophagoides farinae with FEV1 < 80%. 5. Subjects with extrinsic bronchial asthma, sensitive to Dermatophagoides pteronissinus and Dermatophagoides farinae in a state of clinical rest. After 9 minutes of activation, the following results were found, with a significance of p < 0.01: There are significant differences between the normal group and those that we consider the active groups, asthma FEV1 < 80%, pollen-sensitive asthma in springtime and acute asthma. No significant differences exist between the normal group and inactive groups, inactive asthma, pollen-sensitive asthma out of springtime and acute asthma inactive. Significant differences exist in active groups (acute asthma and pollen-sensitive asthma in springtime) when they become inactive (acute asthma inactive and pollen-sensitive asthma out of springtime). The active groups have a higher plasma kallikrein amidolytic activity than both the inactive and control groups.
The study cellular subpopulations in peripheral blood from a normal reference group population (blood donors).
The spectacular development of monoclonal antibodies against cellular antigens an technology such as flow cytometry allow the investigation of cellular subpopulations that until now have been unknown. At the same time, the functional study of these subpopulations becomes of maximal interest, as this information could have future applications for pathological processes. Due ti this basic need for information, we have studied diverse lymphocytic subpopulations in a normal population that serves as a reference group, using the following antigens: CD3, CD4, CD8, CD20, CD45RA, CD25, LAM1, CD29, CD11b and CD23. Some of these subpopulations had not been previously studied in a normal reference group.
[The prevention of cancer and the European Week against Cancer].
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