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J Cornbleet

Publications and source records attributed to J Cornbleet.

14 recordsLinked to original sources
PubMed1996

Streamline your automated hematology laboratory. Roundtable discussion.

How three hematology laboratories improved efficiency by automating the preanalytical process, reorganizing lab workspace, using data management systems to identify which samples need further verification, and setting strict criteria limiting the need for nonautomated processes.

Academic Medical Centers↗
PubMed1992

Expression of multidrug resistance gene mdr1 mRNA in a subset of normal bone marrow cells.

The multidrug resistance gene mdr1, encoding P-glycoprotein (P-gp), can be expressed at high levels in tumour cells derived from normal tissues with constitutive high expression of this gene. In myelogenous leukaemia, the incidence of increased expression of mdr1 gene contrasts with the low expression of this gene in normal bone marrow (b.m.). To detect cells expressing mdr1 gene in normal and post-chemotherapy b.m., we used in situ RNA hybridization and RNA phenotyping by the polymerase chain reaction for mdr1 mRNA detection. The presence of P-gp was evaluated by immunocytochemistry with MRK16. Fifteen b.m. (eight normal and seven post chemotherapy) were tested by in situ hybridization and either PCR (three b.m.) or immunocytochemistry (11 b.m.) or both (one b.m.). With in situ mRNA hybridization, a subset (7.7% +/- 3.1%) of b.m. cells expressed mdr1 mRNA in all cases tested, with no significant differences between normal b.m. and post chemotherapy b.m. 18% of myeloid recognizable cells and 7% of the cells with lymphoid morphology expressed mdr1 mRNA. By RNA phenotyping, the four samples tested for in situ hybridization and two additional post chemotherapy b.m. expressed mdr1. MRK16 was unable to detect a significant number of cells expressing P-gp either by immunocytochemistry in the 12 b.m. tested for in situ hybridization (0% in nine cases; 0.4%, 1% and 3% of positive cells in three cases), or by flow cytometry in six additional normal b.m. (0-1.4% positive cells).

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1985

Evaluation of Coulter S-Plus three-part differential in population with a high prevalence of abnormalities.

The authors evaluated the Coulter S-Plus three-part differential and compared it with the total of component cells obtained by manual slide differential. The patient population (N = 989) was strikingly abnormal, in that 109 samples contained nucleated red blood cells, 118 samples contained one or more abnormal cells (blasts, promyelocytes, or lymphoma cells), and 95 samples had greater than 10% monocytes. The GRAN and LYMPH fractions were accurately measured in the specimens that did not display region (R) flags. The MONO fraction correlated poorly with the manual differential and underestimated monocytes when they were increased. Not all samples with small numbers of abnormal cells or nucleated red blood cells were flagged by the three-part differential. Specimens with eosinophilia and reactive lymphocytosis likewise did not always show R-flags. The following findings (prevalence = 23.9%) were arbitrarily defined to constitute an "abnormal" manual differential: nrbc greater than 0%, blasts greater than 0%, promyel greater than 0%, lymphoma cells greater than 0%, baso greater than 5%, myel greater than 5%, meta greater than 5%, eos greater than 10%, reactive lymphs greater than 10%. Sensitivity of R-flagging for these abnormalities was 81.7% and specificity 73.3%. The predictive value of an R-flagged ("positive") result was 48.3% and of an unflagged ("negative") result, 93.0%. Efficiency (accuracy) was calculated to be 75.5%.

Evaluation Studies as Topic↗