Biomedical subjects

J Czubak

Publications and source records attributed to J Czubak.

10 recordsLinked to original sources

PubMed2000

Rhodanese (thiosulfate:cyanide sulfurtransferase) from frog Rana temporaria.

The molecular mass of rhodanese from the mitochondrial fraction of frog Rana temporaria liver, equaling 8.7 kDa, was determined by high-performance size exclusion chromatography (HP-SEC). The considerable difference in molecular weight and the lack of common antigenic determinants between frog liver rhodanese and bovine rhodanese suggest the occurrence of different forms of this sulfurtransferase in the liver of these animals.

Animals↗

PubMed1998

Ultrasound measurements of the newborn hip. Comparison of two methods in 657 newborns.

We compared ultrasound measurements using the Graf and Terjesen methods in 657 newborns. The alpha angle and femoral head coverage (FHC) were analyzed. The rate of DDH was 3.9%, according to Graf and 2.9%, according to Terjesen. The spontaneous increases in alpha angle and FHC were 5 degrees and 7%, respectively, during the first 2 months. Good accordance between the two methods was shown. A few hips were normal, according to one method and were subluxated, according to the other one. The methods gave similar results, except the percentage of "immature hips" IIa (29%) and "possible dysplastic hips" (14%). This might be a sign of better specificity of the Terjesen method. Good interosberver agreement and simple classification favor use of the Terjesen method. The method of Graf is the most commonly used and gives adequate evaluation of the hip, if the method of examination and rather complicated classification are followed closely.

Acetabulum↗

PubMed1998

Hamstring shortening: postural defect or congenital contracture.

The clinical appearance of hamstring shortening in 10 patients referred with diagnosis of asymmetry of the trunk, scoliosis, postural defect, strange gait, and back pain is reported. Additionally, an assessment of knee extension deficit (KED) angle in 575 healthy children (4-15 years of age) was performed. The mean KED angle in the group of healthy children was 35 degrees (SD = 14 degrees). The border between norm and disease was defined as a mean + 2 SD and equaled 63 degrees. A KED angle greater than 60 degrees occurred in 10% of normal children. There was no correlation between age and a value of KED angle. In the study group, KED angle greater than 40 degrees was more frequent in boys. A correlation between disorders within musculoskeletal system and greater KED angle was found. These disorders were more frequent in children with asymmetrical KED angle. In our opinion, the border value of the KED angle is not a good differentiating factor between hamstring shortening and contracture because it does not explain the cause of the disorder.

Adolescent↗

PubMed1996

Selective effect of 2-(polyhydroxyalkyl)-thiazolidine-4-carboxylic acids on nonprotein sulfhydryl groups in tumor bearing mice.

1. Thiazolidine derivatives (TD), the products of condensation of L-cysteine (cys) with sugars (D-glucose, D-xylose, D-arabinose, D-galactose, and D-mannose), successfully elevated nonprotein sulfhydryl (NPSH) levels in livers of Ehrlich ascites tumor (EAT)-bearing mice. 2. At the same time, TD promoted a significant drop of NPSH in EAT cells. 3. Thus, TD, through their selective influence on the levels of NPSH in liver and cancer cells appear to be promising compounds for anticancer therapy.

Animals↗

PubMed1994

[Multilevel osteotomy with Rush pin fixation in treatment of osteogenesis imperfecta].

Fourteen multilevel osteotomies with Rush pin fixation have been performed in 5 children with osteogenesis imperfecta. The age of patients at the operation ranged from 6.5 to 15.5 years. Plaster of Paris immobilization never exceeded 8 weeks. An axial correction, improved ambulating and equalization of the limbs have been achieved in all cases. Complications of the method included varus deformity of the proximal tibia (1 case), migration of the pin (1 case) and supracondylar fracture of the femur at the level of pin end (2 cases).

Adolescent↗

PubMed1993

Transamination and transsulphuration of L-cysteine in Ehrlich ascites tumor cells and mouse liver. The nonenzymatic reaction of L-cysteine with pyruvate.

1. The activity of cysteine aminotransferase (CAT), 3-mercaptopyruvate sulfurtransferase (MPST) and rhodanese is much lower in Ehrlich ascites tumor cells (EATC) than in mouse liver. 2. Contrary to mouse liver homogenate, no synthesis of sulphane sulphur-containing compounds from L-cysteine is observed in EATC homogenate. 3. 2-Methyl-thiazolidine-2,4-dicarboxylic acid (CP), 2-methyl-thiazolidine-4-carboxylic acid (CA) and thiazolidine-4-carboxylic acid (CF) can be used as sources of low molecular-weight thiol compounds both in EATC and mouse liver homogenate. 4. Pyruvate formed from phosphoenolpyruvate (PEP) in EATC homogenates reacts with L-cysteine (L-CYS) to CP.

Amines↗

PubMed1984

Formation of 2-methyl-2,4-thiazolidinedicarboxylic acid from L-cysteine in rat tissues.

The adduct formed non-enzymatically from L-cysteine and pyruvate: 2-methyl-2,4-thiazolidinecarboxylic acid (CP) was isolated, and identified by the electron impact mass spectroscopy. It was found that CP is formed (by cysteine transformation) and is metabolized in rat tissues. Formation of CP from cysteine or cystine was catalysed by partially purified rat liver gamma-cystathionase.

Animals↗

PubMed1983

Phosphoprotein phosphatase activity of human prostate acid phosphatase.

Human prostate acid phosphatase (EC 3.1.3.2) has been shown to dephosphorylate different phosphoproteins with the maximum rate at pH 4.0-4.5. The activity with phosvitin is distinctly higher than with beta-casein, casein and most of all than with riboflavin-binding protein. The native phosvitin is homogeneous on isoelectric focusing with pI value of 2.1, whereas phosvitin partially dephosphorylated (in about 15%) by the prostate acid phosphatase shows multiple bands with pI values of 3.5 - 6.8 or higher. The phosphate groups bound to serine residues are removed enzymatically twice as fast as phosphothreonine residues. The apparent Km value for phosvitin was 2.4 X 10(-7) M, and is by three orders of magnitude lower than Km of p-nitrophenyl phosphate (2.9 X 10(-4) M). The competitive inhibitors of prostate acid phosphatase, fluoride and L(+)-tartrate, show the same Ki values for phosvitin and p-nitrophenyl phosphate.

Acid Phosphatase↗

PubMedDate not supplied

Repair of articular cartilage defects with cultured chondrocytes on polysulphonic membrane: experimental studies in rabbits.

INTRODUCTION: Autologous osteochondral transplantation is one method that can be used to create hyaline or hyaline-like repair in a defect area. The purpose of the present study was to repair full-thickness articular cartilage defects in 9 rabbit knee joints with autologous cultured chondrocytes. METHODS: An articular cartilage defect was created on the patellar groove of the femur. The defect was filled with chondrocytes cultured in vitro and placed into the knee on a polysulphonic membrane. At 8 weeks after the operation, the reparative tissue was analyzed macroscopically and histologically. RESULTS: At 8 weeks after the operation, the surfaces of the reparative tissue were smooth, and the defects were filled with mature hyaline cartilage in 5 cases. In 2 cases, the reparative hyaline cartilage was immature and there was worse integration of grafted tissue into the adjacent normal cartilage. In 2 cases, the surface of the grafted area was irregular, and the reparative tissue was disintegrated and incompletely differentiated. CONCLUSION: The results suggest that transplantation of autologous chondrocytes cultured in vitro and placed into the knee on polysulphonic membrane is effective in repairing an articular cartilage defect.

Animals↗

PubMedDate not supplied

The antioxidative properties of thiazolidine derivatives.

Antioxidative properties of thiazolidine compounds, adducts of L-cysteine with formaldehyde (thiazolidine-4-carboxylic acid), with pyruvate (2-methyl-thiazolidine-2,4-dicarboxylic acid), and glucose (2-/D-gluco-1',2',3',4',5'-penthahydroxypentyl/- thiazolidine-4-carboxylic acid) were studied. The investigated compounds, as well as L-cysteine and DL-penicillamine inhibited in vitro lipid peroxidation induced by NADPH, ethanol, and hydrogen peroxide in the suspension of rat liver microsomes.

Animals↗