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Biomedical subjects

J D Dodge

Publications and source records attributed to J D Dodge.

14 recordsLinked to original sources

Burns follow-up: an innovative application of telemedicine.

We assessed the efficiency and effectiveness of burn consultations via telemedicine. The Regions Hospital Burn Center completed 87 follow-up visits with 40 patients via telemedicine from March 1997 to August 1998. These consultations involved burn physicians, occupational therapists and a clinical psychologist. Patients were seen at 15 telemedicine sites in six states (Minnesota, Iowa, Montana, North and South Dakota, and Wisconsin). Telemedicine burn consultations were cost-effective for the patient, but were more time consuming for the physician and therapist. As remote sites become more familiar with preparing patients for teleconsultations, telemedicine will become more efficient for the physician while remaining cost-effective for the patient.

Adolescent↗

Nuclear and plastid DNAs from the binucleate dinoflagellates Glenodinium (Peridinium) foliaceum and Peridinium balticum.

The binucleate dinoflagellates Glenodinium (Peridinium) foliaceum Stein and Peridinium balticum (Levander) Lemmermann were found to contain two major buoyant density classes of DNA. The heavier peak (1.730 g/cm3) was derived from the "dinokaryotic" nucleus and the lighter peak (1.706 g/cm3) from the "endosymbiont" nucleus and this allowed for the fractionation of G. foliaceum DNA in CsCl/EtBr density gradients. An initial CsCl/Hoechst Dye gradient removed a minor A-T rich satellite species which was identified as plastid DNA with a size of about 100-106 kb. Analysis of the nuclear DNA by agarose gel electrophoresis and renaturation studies showed that the endosymbiont nucleus lacked amplified gene-sized DNA molecules, however, this nucleus did have a comparatively high level of DNA. The total amount of DNA per cell and the relative contributions of the two nuclei appeared to vary between two strains of G. foliaceum (75 pg/cell in CCAP strain and 58 pg in UTEX strain). The only strain of P. balticum examined contained 73 pg cell. These results are discussed in relation to the status and possible functioning of the endosymbiont nucleus and the idea that these dinoflagellates provide model systems with which to study the evolution of plastids.

Animals↗

Mitosis and cytokinesis in the dinoflagellate Amphidinium carterae.

Mitosis and cytokinesis are described in the free-living dinoflagellate, Amphidinium carterae Hulburt. In prophase, invaginations of the nuclear envelope form into tunnels which enclose bundles of microtubules. No centrioles or spindle organizers are present but microbodies are situated at the ends of the bundles of microtubules. The dividing chromosomes are attached to the tunnels, and microtubules connect to the nuclear envelope adjacent to the chromosomes by means of kinetochore-like structures. At anaphase, daughter chromatids are pulled apart as the nucleus extends but there is little change in the pole to chromosome distance. Cytokinesis is brought about by pinching-in of the cytoplasm adjacent to the equatorial region in an area where many microtubules lie under the plasmalemma. The account adds further details to the earlier descriptions of mitosis in other dinoflagellates.

Animals↗

The functional and phylogenetic significance of dinoflagellate eyespots.

The eyespots or stigmas from five species of dinoflagellates fall into three distinct categories: independent eyespot which is not membrane bound; independent eyespot which is surrounded by three membranes; eyespot situated at the periphery of a chloroplast. In all cases the eyespot is situated behind the longitudinal groove or sulcus and there is a strand of microtubules between the eyespot and the cell covering or theca. In two cases the strand has been clearly shown to originate near the base of the longitudinal flagellum, which is the one passing over the eyespot and is also responsible for directional movement of the cell. The microtubular strand is presumed to play a part in the transmission of directional stimulation from eyespot to flagellum and a hypothesis is advanced to explain how this may be brought about. Phylogenetically, the structure of the various types of eyespots would link these dinoflagellates with euglenids and chrysophytes , and the diversity found in the dinoflagellates is probably a reflection of the diverse origin of chloroplasts in this group.

Animals↗