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J D Goosey

Publications and source records attributed to J D Goosey.

23 records · Page 2Linked to original sources
PubMed1981

Effects of singlet oxygen on human lens crystallins in vitro.

We have recently demonstrated that singlet oxygen-mediated photooxidation can cause an increase in blue fluorescence and covalent cross-linking in bovine lens crystallins in vitro. Because these changes closely parallel modifications known to occur in human crystallins during aging and cataractogenesis, it was suggested that singlet oxygen may play an important role in these processes in vivo. to confirm these results, obtained with photosensitizers free in solution, we now report results from experiments with a polymer-bound photosensitizer and with photophysically generated singlet oxygen. Singlet oxygen photodynamically generated by polymer-immobilized rose bengal produced the following modifications to human lens crystallins in vitro: covalent cross-linking, increased blue fluorescence, yellow pigmentation, and formation of heavy-molecular-weight aggregates. Singlet oxygen generated photophysically was shown to cause an increase in blue fluorescence and covalent cross-linking in human crystallins. These findings substantiate the hypothesis that singlet oxygen may play an important role in the changes seen in human lens proteins during aging and cataractogenesis.

Aging↗
PubMed1980

Cross-linking of lens crystallins in a photodynamic system: a process mediated by singlet oxygen.

In a dye-sensitized photooxidation system, lens crystallin polypeptides become cross-linked, and a blue fluorescence that is associated with the proteins is produced. These changes are similar to those seen in vivo in the aging human lens. Evidence implicating singlet oxygen as the causative agent of the effects in vitro is presented, and the possibility that this species may play a role in aging and cataractogenesis in vivo is discussed.

Aging↗
PubMedDate not supplied

Epikeratophakia on pediatric traumatized corneas.

Four children ranging from 4 to 6 years of age with unilateral aphakia and corneal scarring secondary to penetrating injuries were treated with epikeratophakia. One child had undergone a rotational penetrating keratoplasty to displace the corneal scar from the visual axis prior to the epikeratophakia procedure. In all of the cases, nonlyophilized donor tissue was utilized. The procedure was successful in all four cases. The average best corrected postoperative visual acuity was 20/45. These results confirm prior studies suggesting that epikeratophakia can be performed successfully on partially opaque and scarred corneas in pediatric aphakic eyes.

Child, Preschool↗
PubMedDate not supplied

Stability of refraction during two years after myopic epikeratoplasty.

A series of 54 consecutive myopic epikeratoplasty procedures was performed by three surgeons between November 1986 and August 1987. To evaluate the stability of the myopic epikeratoplasty procedure, this study was limited to the 33 eyes that had data 24 months after surgery. One year after surgery, 68% (19/28 eyes) had 20/40 or better uncorrected visual acuity and after two years, 57% (17/30 eyes) had 20/40 or better uncorrected visual acuity. Significant loss of surgical effect (increasing myopia) did not occur during the first six postoperative months (r = 0.09, P = NS). This was due to the large amount of variability in spherical equivalent refraction during this interval. However significant regression was detected between 6 and 12 months after surgery (r = 0.27, F = 4.74, P = 0.03). Even though there was further reduction in variability from 12 to 24 months, significant regression was not found (r = 0.07, P = NS). This suggests stabilization of refraction by approximately 12 months for this population.

Adolescent↗