Biomedical subjects

J D More

Publications and source records attributed to J D More.

3 recordsLinked to original sources

PubMed1976

Application of immunofluorescent staining on paraffin sections improved by trypsin digestion.

The purpose of this study was to explore a method by which an improved immunofluorescent staining can be applied to formalin-fixed paraffin sections to demonstrate cellular or tissue deposits of immunoglobulins, complement and fibrin, and to demonstrate alpha-1-antitrypsin storage and hepatitis B antigens in liver, toxoplasma in heart, and carcinoembryonic antigens in colonic cancer. It was shown that immunohistochemical demonstration for the above mentioned antigens, but not for complement, was feasible. The paraffin sections were first treated with trypsin and the indirect staining method was used. The trypsin treatment was found to decrease the nonspecific background fluorescence through digestion of the tissue. It probably also unmasked the immunoreactive sites of viral antigens and alpha-1-antitrypsin. In general, a 2-hour digestion was satisfactory for the types of tissues examined in this study, and an optimal period of digestion could be sought to obtain the best result for a specific antigen. This method may be a useful adjuvant to histopathologic study, in which a retrospective immunohistochemical examination may be desirable.

Antigens, Viral↗

PubMedDate not supplied

Integrating academic and community laboratory needs: developing a successful outreach program.

Remarkably few large regional laboratory programs involving academic health centers exist in Canada. We present a model of a successful laboratory outreach program established by the Queen's University Department of Pathology (Kingston, Ontario) and its affiliated hospital laboratories. Community hospitals in small urban and semi-rural areas benefit from a diffusion of specialty expertise in laboratory medicine and technology, which enables them to increase their local testing capabilities and improve turn-around time. Testing and services of a more complex nature are referred to the academic hospitals. The result has been a steady improvement in the quality of laboratory services in the region with increased cost-effectiveness through shared services and focused economies of scale.

Academic Medical Centers↗

PubMedDate not supplied

Promoting, building and sustaining a regional laboratory network in a changing environment.

The Queen's University Department of Pathology and its affiliated hospital laboratories (Kingston, Canada) have operated a successful laboratory outreach program for more than a decade in Southeastern Ontario. The outreach program provides high quality reference testing and technical and professional expertise in laboratory medicine to largely rural and small urban community hospitals. As a consequence of dramatic cuts to the publicly funded health-care system in the Province of Ontario, the environment in which laboratory medicine is practiced has altered irrevocably. This article discusses some of the difficult internal and external challenges faced by the outreach program within the region and how they were effectively managed, not only to maintain but to enhance the program's services. The result has been a continued improvement in the quality of laboratory services in the region with significantly increased cost-effectiveness, largely through reengineering and consolidation.

Budgets↗