Biomedical subjects

J D Patrick

Publications and source records attributed to J D Patrick.

5 recordsLinked to original sources

PubMed1994

Evaluation of immunoperoxidase techniques to detect Rickettsia rickettsii in fixed tissue sections.

Standard immunoperoxidase techniques were evaluated in the diagnosis of Rocky Mountain spotted fever (RMSF). Formalin-fixed, paraffin-embedded tissue was tested to detect Rickettsia rickettsii using the same antibody provided by the Centers for Disease Control and Prevention that is used for direct immunofluorescence (DIF). Tissues from 23 patients with suspected RMSF were divided: some were snap-frozen for DIF; the remainder were fixed in formalin, processed routinely, and embedded in paraffin for immunoperoxidase and hematoxylin and eosin staining. Ten patients were ultimately determined to have RMSF; in nine of these patients, both DIF and immunoperoxidase staining were positive for R rickettsii. There were no discrepancies, positive or negative, between the two methods. In the one case with a false-negative result by both methods, the patient had received antirickettsial antibiotics 72 hours before the biopsy was performed. These data demonstrate that the immunoperoxidase technique is effective in diagnosing RMSF and may be applied to cases retrospectively.

Adolescent↗

PubMed1987

Acute arsenic ingestion.

A 21-year-old man presented in shock after ingesting 2 g of arsenic trioxide. He died within 37 hours despite intensive treatment that included intramuscular dimercaprol and hemodialysis. Hemodynamic and laboratory data are presented illustrating the multisystem toxicities of inorganic arsenic. Hemodialysis, previously described as an effective therapeutic adjunct, was shown to be ineffective in this case.

Acute Kidney Injury↗

PubMed1983

Lumbar spine x-rays: a multihospital study.

To define the usefulness of the lumbar spine x-ray series in the emergency department and to generate clinical criteria for optimizing its application, we retrospectively studied 552 consecutive emergency department patients for whom lumbar spine x-rays were ordered. Patients were divided into traumatic (47.6%) and nontraumatic (52.4%) groups. Three subgroups were created based on radiological findings: 1) "negative" (55.8%), 2) "possibly significant" (37%), and 3) "positive" (7.2%). The "positive" subgroup was compared with the other two subgroups in an attempt to define physical markers that correlated with positive radiological findings. Four clinical findings were present in significantly different frequencies between the positive group and others: an abnormal physical examination (90% vs 61.5%, respectively) (P less than .0001), tenderness (72.5% vs 41.2%) (P less than .0005), multiple positive findings (42.5% vs 20.7%) (P less than .005), and contusion or abrasion (15% vs 2.7%) (P less than .0005).

Adolescent↗

PubMed1982

Ectopic pregnancy--a brief review.

Despite the advent of improved methods of diagnosis and therapy, ectopic pregnancy remains a significant cause of morbidity and mortality worldwide. The diagnosis is often difficult to make clinically, but should be included in the differential of any woman of gestational age presenting with lower abdominal pain, even with a normal menstrual history. With a high index of suspicion, improving methods of diagnosis such as the immunoassays for B-hCG and ultrasonography may lower mortality rates by enabling more diagnoses to be made before rupture. The significant infertility rate following ectopic pregnancy remains a problem despite newer, more conservative approaches to surgical therapy.

Culdoscopy↗

PubMedDate not supplied

Position effect variegations and brain-specific silencing in transgenic mice overexpressing human acetylcholinesterase variants.

Position effect variegations as well as brain-specific silencing were observed in novel transgenic mouse pedigrees expressing human acetylcholinesterase (AChE) variants. Muscle AChE activities varied between 1.6- and 350-fold of control in these lines, one carrying insertion-inactivated InE6-AChE and two with 'readthrough' I4/E5 AChE, all under control of the ubiquitous CMV promoter. In contrast, brain AChE levels remained within a range of 1.5-fold over control, suggesting an upper limit of brain AChE which is compatible with life.

Acetylcholinesterase↗