A convenient method for assay of DNA synthesis in synchronized human cell cultures.
Explore the source record for details and available documents.
Autoradiography↗
Biomedical subjects
J D Regan
Publications and source records attributed to J D Regan.
Triploidy in human cell line.
Cytogenetic studies of a human amnion cell line (strain RA) indicate that the major stem cell was triploid. Consistently present in the triploid cells were two marker chromosomes, a small centric fragment, and an apparently telocentric D. At various times triploid cells constituted 40 to 80 percent of the cell population. The possible origin of the triplaid cell is a diploid/ triploid mosaic amnion.
Amnion↗
Quantitative estimation of the extent of alkylation of DNA following treatment of mammalian cells with non-radioactive alkylating agents.
Alkaline sucrose sedimentation has been used to quantitate phosphotriester formation following treatment of human cells with the monofunctional alkylating agents methyl and ethyl methanesulfonate. These persistent alkaline-labile lesions are not repaired during short-term culture conditions and thus serve as a useful and precise index of the total alkylation of the DNA. Estimates of alkylation by this procedure compare favorably with direct estimates by use of labeled alkylating agents.
Alkylating Agents↗
Repair endonuclease-sensitive sites in daughter DNA of ultraviolet-irradiated human cells.
Explore the source record for details and available documents.
Binding Sites↗