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Biomedical subjects

J Daszyński

Publications and source records attributed to J Daszyński.

At least 19 recordsLinked to original sources
PubMed1979

Influence of stroma-free hemoglobin solution on renal function in dogs.

In the presented series of experiments, stroma-free hemoglobin solution (SFHS) was used as a perfusate for extracorporeal perfusion of kidneys and for exchange transfusion. It was shown that SFHS was adequatly oxygenated both as perfusate during the extracorporal perfusion and in the lungs after the blood exchange. Furthermore, there was provided evidence that oxygen was being transported and released in the tissues. Histologic examination of the kidneys demonstrated hemoglobin casts in the glomerules. Most of them were seen after extracorporal perfusion of isolated kidneys, less frequently after perfusion in situ and few casts were present after exchange transfusion. The cause of these depositions may be small remnants of stroma in the preparation. Furthermore it is possible, that at the beginning of the extracorporal perfusion of the kidneys the urine pH was below 6 and then a hemoglobin casts formation could occur. There were essential no changes in urine pH in the course of blood exchange. In this group were only few casts present in the kidneys.

Animals↗
PubMed1977

Infusion of physiological saline supplemented with adenine, pyruvate and phosphate.

After controlled bleeding 20 ml/kg enriched infusion fluid containing 0.005 mol/1 adenine, 0.1 mol/1 pyruvate, 0.1 mol/1dibasic sodium phosphate and 0.15 mol/1 sodium chloride was given to 14 dogs. Three dogs received after bleeding the same infusion fluid without adenine. One dog received only dextran after blood withdrawal and served as control Before and after bleeding and 1, 4, 24, 48 and 72 hours after infusion the levels of 2,3-DPG, ATP and P50 index were determined. After infusion of enriched fluid with and without adenine the level of 2,3-DPG and the value of P50 index increased. No rise in the ATP level was observed.

Adenine↗
PubMed1976

Effects of stroma-free haemoglobin solution on the blood-clotting system in dogs.

The effect of transfusions of 500 ml of 5-6% stroma-free haemoglobin solutions on the blood-clotting system of dogs was studied. Slight lowering of the fibrinogen level and of the activity of clotting factors V, VII, VIII, IX and X was observed, together with some prolongation of the thrombin time. In vitro investigations indicate that prolongation of the thrombin time may depend on disturbed polymerization of fibrin monomers. After transfusion of stroma-free haemoglobin preparation, no signs of haemorrhagic diathesis were observed.

Animals↗
PubMed1976

[Erythrocyte freezing in the presence of hydroxyethyl starch].

Biochemical properties of erythrocytes frozen in the presence of hydroxyethyl starch (HES) as preserving substance produced in this country were assessed. Erythrocytes were frozen adding HES in 4 different concentrations. The most favourable results were obtained with erythrocytes subjected to the action of HES in 30% and 35% concentrations. The erythrocyte 2,3-DPG level during freezing with 35% HES was within normal limits while it decreased by 10% after defreezing when 30% HES had been used. The ATP concentration in erythrocytes fell by 50% in relation to the initial value. A slight fall of P50 was observed and erythrocyte loss after defreezing did not exceed 10%. The 35% HES solution in a 0.15 mol/l sodium chloride solution was most favourable for freezing erythrocytes in liquid nitrogen. A simple method of preparation of defrozen erythrocytes for transfusion was elaborated.

Adenosine Triphosphate↗
PubMed1976

Experimental investigations on the possibility of transplantation of venous grafts stored at low temperatures.

At the Laboratory of Experimental Surgery, Institute of Haematology in Warsaw 13 dogs received homografts of venous patches stored at low temperatures to fill defects in the wall of the lower caval vein. Venous patches were prepared from segments of femoral veins and were placed in 1.41 M DSMO and stored at the temperature of liquid nitrogen. The authors describe the methods of obtaining venous patches, the technique of freezing and defreezing of grafts, and details of operations. Full healing in of grafts took about 15 days. The results of histological examinations of grafts obtained during planned autopsies between 12 and 29 days after application of the graft are discussed.

Animals↗
PubMed1976

Oxygen consumption by hepatic tissue after transfusion of a stroma-free haemoglobin solution.

The ability of stroma-free haemoglobin solutions to transport oxygen was investigated by determining the oxygen consumption by the liver using Warburg's microrespirator. 50 ml of blood were removed from the rabbit under general anaesthesia and replaced by an identical volume of a non-oxygenated or oxygenated haemoglobin solution. The control rabbits received no transfusions. It was found that arterial hypotension produced by blood-letting caused a significant rise in oxygen consumption by the hepatic tissue. The rise was increased even more when the rabbits received no transfusions. Transfusion of non-oxygenated haemoglobin solutions caused likewise a rise in oxygen consumption immediately after transfusion. This rise was, however, not so significant as in the control group. On the other hand, transfusion of oxygenated haemoglobin solutions produced no rise in the oxygen consumption by the liver as compared to its value after blood-letting.

Animals↗
PubMedDate not supplied

[Effect of stroma-free hemoglobin solution on oxygen consumption in some tissues].

50 cc of a 6%, oxygenated or non oxygenated stroma-free heamoglobin solution was transfused in rabbits after previous removal of about one third of the circulating blood volume. The experimental investigations were carried out on animals under barbiturate anaesthesia. Oxygen consumption was determined using a Warburg microrespirator. Posthaemorrhagic hypotension developing after bleeding caused a significant increase in oxygen consumption by liver tissue. Transfusion of non oxygenated stroma-free haemoglobin solution caused a further increase of oxygen consumption, while transfusion of oxygenated stroma-free haemoglobin solution slightly decreased oxygen consumption compared to the values observed during the post haemorrhagic hypotension.

Animals↗
PubMedDate not supplied

[Behavior of zinc and magnesium in stored blood].

An analytical method was elaborated for determination of blood zinc and magnesium based on previously known methods. Blood zinc and magnesium were determined in whole stored blood, plasma and erythrocyte mass. The results obtained by this method were compared with the results of spectrophotometry of atomic absorption (SAA). They showed a rise in the plasma zinc and magnesium levels in blood stored with ACD at +4 degrees -- +6 degrees C. during three weeks.

Blood Preservation↗