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J Dijkhof

Publications and source records attributed to J Dijkhof.

6 recordsLinked to original sources
PubMed1978

Non-parallel secretion of newly synthesized rat pancreatic proteins after feeding a diet containing raw soybean flour.

The relative rate of secretion of rat pancreatic proteins was studied in vivo using a double label method. Rats were injected with [3H]leucine and after different time intervals with [14C]leucine. At a fixed time after administration of the second precursor the animals were killed, and the pancreatic proteins were separated by polyacrylamide gel electrophoresis. The dpm of tritium to dpm of 14C ratio of several identified enzymes was assessed. The percentage secretion of a newly synthesized secretory protein was derived from the difference between the actual 3H/14C ratio and the 3H/14C ratio that was found for non-secretory proteins. In pancreata of rats fed with a standard diet several identified proteins, viz. three trypsinogens, chymotrypsinogen and three amylases were secreted in "parallel". When a diet containing raw soybean flour was fed, the secretory pattern for the amylases differed from that of the other proteins.

Amylases↗
PubMed1978

Changes in rat pancreatic protein synthesis after a single feeding with diets containing raw or heated soybeans.

The patterns of protein synthesis following the administration of a single meal containing defatted ground soybeans (RSD) or heated defatted ground soybeans (HSD) were compared. A double label method was used so that the determination of the relative rate of synthesis of an enzyme could not be obscured by a possible alteration of the activity or quantity of the enzyme. Rats were given one meal of RSD or HSD and were subsequently killed at different times after the meal. Eight hours after the meal, the relative rate of synthesis of one of the three trypsinogens was substantially increased with RSD feeding, whereas that of the amylases and one chymotrypsinogen were somewhat lower. The synthetic rate of lipase, ribonclease, proelastase, another chymotrypsinogen and of two trypsinogens was unaffected when feeding RSD is compared to feeding HSD. The relative rate of synthesis of one of the trypsinogens was unaffected 8 hours after RSD feeding, but was increased 16 hours after RSD feeding. Actinomycin D could suppress the effects of RSD feeding on the protein synthetic rate of some, but not of all, secretory proteins.

Animals↗